Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2117 and GFF2118 are separated by 182 nucleotides GFF2118 and GFF2119 are separated by 72 nucleotides GFF2119 and GFF2120 are separated by 2 nucleotides
GFF2117 - RND efflux system, membrane fusion protein CmeA, at 4,658 to 5,815
GFF2117
GFF2118 - FIG01046338: hypothetical protein, at 5,998 to 6,156
GFF2118
GFF2119 - Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family, at 6,229 to 6,891
GFF2119
GFF2120 - FIG00554530: hypothetical protein, at 6,894 to 8,993
GFF2120
Position (kb) 6
7 Strain fitness (log2 ratio) -1
0
1 at 5.231 kb on - strand, within GFF2117 at 5.268 kb on - strand, within GFF2117 at 5.269 kb on + strand, within GFF2117 at 5.301 kb on + strand, within GFF2117 at 5.311 kb on + strand, within GFF2117 at 5.314 kb on + strand, within GFF2117 at 5.314 kb on + strand, within GFF2117 at 5.409 kb on + strand, within GFF2117 at 5.494 kb on + strand, within GFF2117 at 5.506 kb on - strand, within GFF2117 at 5.517 kb on + strand, within GFF2117 at 5.587 kb on + strand, within GFF2117 at 5.587 kb on + strand, within GFF2117 at 5.772 kb on - strand at 6.405 kb on + strand, within GFF2119 at 6.896 kb on - strand at 6.909 kb on - strand at 7.054 kb on - strand at 7.080 kb on + strand at 7.198 kb on + strand, within GFF2120 at 7.213 kb on + strand, within GFF2120 at 7.434 kb on + strand, within GFF2120 at 7.435 kb on - strand, within GFF2120 at 7.603 kb on - strand, within GFF2120 at 7.607 kb on + strand, within GFF2120 at 7.608 kb on - strand, within GFF2120 at 7.771 kb on + strand, within GFF2120 at 7.771 kb on + strand, within GFF2120 at 7.881 kb on - strand, within GFF2120 at 7.881 kb on - strand, within GFF2120
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 5,231 - GFF2117 0.49 +1.0 5,268 - GFF2117 0.53 +0.2 5,269 + GFF2117 0.53 -0.5 5,301 + GFF2117 0.56 -0.2 5,311 + GFF2117 0.56 -0.2 5,314 + GFF2117 0.57 -0.9 5,314 + GFF2117 0.57 -0.0 5,409 + GFF2117 0.65 -0.5 5,494 + GFF2117 0.72 -0.5 5,506 - GFF2117 0.73 -0.1 5,517 + GFF2117 0.74 +0.9 5,587 + GFF2117 0.80 -1.0 5,587 + GFF2117 0.80 -0.7 5,772 - +0.1 6,405 + GFF2119 0.27 -1.0 6,896 - +0.4 6,909 - +1.0 7,054 - +0.1 7,080 + -1.0 7,198 + GFF2120 0.14 -0.0 7,213 + GFF2120 0.15 -1.6 7,434 + GFF2120 0.26 -1.1 7,435 - GFF2120 0.26 +0.7 7,603 - GFF2120 0.34 -0.2 7,607 + GFF2120 0.34 +1.7 7,608 - GFF2120 0.34 -0.1 7,771 + GFF2120 0.42 +0.6 7,771 + GFF2120 0.42 +0.4 7,881 - GFF2120 0.47 -0.5 7,881 - GFF2120 0.47 -0.6
Or see this region's nucleotide sequence