Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1954

Experiment: LB_plus_SM_buffer with S16_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1952 and GFF1953 are separated by 99 nucleotidesGFF1953 and GFF1954 are separated by 307 nucleotidesGFF1954 and GFF1955 overlap by 1 nucleotides GFF1952 - FIG001674: hypothetical protein, at 143,150 to 143,701 GFF1952 GFF1953 - Phosphohistidine phosphatase SixA, at 143,801 to 144,187 GFF1953 GFF1954 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 144,495 to 146,642 GFF1954 GFF1955 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 146,642 to 147,952 GFF1955 Position (kb) 144 145 146 147Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 143.558 kb on - strand, within GFF1952at 143.694 kb on + strandat 143.879 kb on - strand, within GFF1953at 144.107 kb on + strand, within GFF1953at 144.108 kb on - strand, within GFF1953at 144.409 kb on - strandat 144.425 kb on + strandat 144.425 kb on + strandat 144.457 kb on - strandat 144.838 kb on + strand, within GFF1954at 145.136 kb on + strand, within GFF1954at 145.136 kb on + strand, within GFF1954at 145.137 kb on - strand, within GFF1954at 145.162 kb on + strand, within GFF1954at 145.163 kb on - strand, within GFF1954at 145.200 kb on - strand, within GFF1954at 145.373 kb on + strand, within GFF1954at 145.620 kb on - strand, within GFF1954at 145.623 kb on + strand, within GFF1954at 145.624 kb on - strand, within GFF1954at 145.624 kb on - strand, within GFF1954at 145.624 kb on - strand, within GFF1954at 145.631 kb on + strand, within GFF1954at 145.632 kb on - strand, within GFF1954at 145.632 kb on - strand, within GFF1954at 145.917 kb on - strand, within GFF1954at 145.959 kb on + strand, within GFF1954at 145.991 kb on + strand, within GFF1954at 146.246 kb on + strand, within GFF1954at 146.247 kb on - strand, within GFF1954at 146.421 kb on + strand, within GFF1954at 146.433 kb on - strandat 146.454 kb on - strandat 146.785 kb on - strand, within GFF1955at 146.891 kb on + strand, within GFF1955at 147.006 kb on - strand, within GFF1955at 147.006 kb on - strand, within GFF1955at 147.055 kb on - strand, within GFF1955at 147.265 kb on - strand, within GFF1955at 147.265 kb on - strand, within GFF1955at 147.365 kb on - strand, within GFF1955at 147.365 kb on - strand, within GFF1955at 147.369 kb on + strand, within GFF1955at 147.370 kb on - strand, within GFF1955

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI
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143,558 - GFF1952 0.74 +1.8
143,694 + +1.2
143,879 - GFF1953 0.20 +1.8
144,107 + GFF1953 0.79 +2.0
144,108 - GFF1953 0.79 +2.3
144,409 - -0.1
144,425 + +0.5
144,425 + -0.8
144,457 - -0.2
144,838 + GFF1954 0.16 -0.3
145,136 + GFF1954 0.30 +0.2
145,136 + GFF1954 0.30 -0.8
145,137 - GFF1954 0.30 -0.4
145,162 + GFF1954 0.31 -0.7
145,163 - GFF1954 0.31 +0.0
145,200 - GFF1954 0.33 +0.8
145,373 + GFF1954 0.41 -0.6
145,620 - GFF1954 0.52 -0.6
145,623 + GFF1954 0.53 +0.8
145,624 - GFF1954 0.53 -0.3
145,624 - GFF1954 0.53 +0.1
145,624 - GFF1954 0.53 +0.6
145,631 + GFF1954 0.53 -0.8
145,632 - GFF1954 0.53 -0.7
145,632 - GFF1954 0.53 -0.3
145,917 - GFF1954 0.66 -0.2
145,959 + GFF1954 0.68 -1.1
145,991 + GFF1954 0.70 +0.4
146,246 + GFF1954 0.82 -3.1
146,247 - GFF1954 0.82 -0.2
146,421 + GFF1954 0.90 -0.7
146,433 - +2.7
146,454 - -0.2
146,785 - GFF1955 0.11 -0.7
146,891 + GFF1955 0.19 +0.0
147,006 - GFF1955 0.28 +0.1
147,006 - GFF1955 0.28 -2.2
147,055 - GFF1955 0.32 +0.2
147,265 - GFF1955 0.48 -0.0
147,265 - GFF1955 0.48 +0.7
147,365 - GFF1955 0.55 +0.4
147,365 - GFF1955 0.55 -1.0
147,369 + GFF1955 0.55 -0.2
147,370 - GFF1955 0.56 -0.8

Or see this region's nucleotide sequence