Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1951 and GFF1952 are separated by 165 nucleotides GFF1952 and GFF1953 are separated by 99 nucleotides GFF1953 and GFF1954 are separated by 307 nucleotides
GFF1951 - Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p, at 142,052 to 142,984
GFF1951
GFF1952 - FIG001674: hypothetical protein, at 143,150 to 143,701
GFF1952
GFF1953 - Phosphohistidine phosphatase SixA, at 143,801 to 144,187
GFF1953
GFF1954 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 144,495 to 146,642
GFF1954
Position (kb) 143
144 Strain fitness (log2 ratio) -1
0
1
2 at 142.185 kb on - strand, within GFF1951 at 142.234 kb on - strand, within GFF1951 at 142.356 kb on + strand, within GFF1951 at 142.367 kb on - strand, within GFF1951 at 142.384 kb on - strand, within GFF1951 at 142.441 kb on + strand, within GFF1951 at 142.462 kb on + strand, within GFF1951 at 142.530 kb on + strand, within GFF1951 at 142.531 kb on - strand, within GFF1951 at 142.824 kb on + strand, within GFF1951 at 142.942 kb on + strand at 143.045 kb on + strand at 143.045 kb on + strand at 143.289 kb on + strand, within GFF1952 at 143.351 kb on - strand, within GFF1952 at 143.369 kb on + strand, within GFF1952 at 143.369 kb on + strand, within GFF1952 at 143.558 kb on - strand, within GFF1952 at 143.694 kb on + strand at 143.879 kb on - strand, within GFF1953 at 144.107 kb on + strand, within GFF1953 at 144.108 kb on - strand, within GFF1953 at 144.409 kb on - strand at 144.425 kb on + strand at 144.425 kb on + strand at 144.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 142,185 - GFF1951 0.14 -0.8 142,234 - GFF1951 0.20 -0.1 142,356 + GFF1951 0.33 -0.7 142,367 - GFF1951 0.34 +0.2 142,384 - GFF1951 0.36 +1.4 142,441 + GFF1951 0.42 -0.5 142,462 + GFF1951 0.44 +0.2 142,530 + GFF1951 0.51 -1.1 142,531 - GFF1951 0.51 +0.1 142,824 + GFF1951 0.83 -1.2 142,942 + +0.4 143,045 + +0.1 143,045 + +0.2 143,289 + GFF1952 0.25 +0.2 143,351 - GFF1952 0.36 -0.6 143,369 + GFF1952 0.40 +0.1 143,369 + GFF1952 0.40 +0.4 143,558 - GFF1952 0.74 +1.8 143,694 + +1.2 143,879 - GFF1953 0.20 +1.8 144,107 + GFF1953 0.79 +2.0 144,108 - GFF1953 0.79 +2.3 144,409 - -0.1 144,425 + +0.5 144,425 + -0.8 144,457 - -0.2
Or see this region's nucleotide sequence