Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1949

Experiment: LB_plus_SM_buffer with S16_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1947 and GFF1948 overlap by 1 nucleotidesGFF1948 and GFF1949 overlap by 1 nucleotidesGFF1949 and GFF1950 are separated by 3 nucleotides GFF1947 - Putative transporting ATPase, at 138,746 to 139,294 GFF1947 GFF1948 - Putative membrane protein YfcA, at 139,294 to 140,103 GFF1948 GFF1949 - Murein endopeptidase, at 140,103 to 140,927 GFF1949 GFF1950 - Chorismate synthase (EC 4.2.3.5), at 140,931 to 142,016 GFF1950 Position (kb) 140 141Strain fitness (log2 ratio) -2 -1 0 1 2at 139.228 kb on + strand, within GFF1947at 139.340 kb on + strandat 139.340 kb on + strandat 139.341 kb on - strandat 139.441 kb on + strand, within GFF1948at 139.572 kb on + strand, within GFF1948at 139.573 kb on - strand, within GFF1948at 139.583 kb on - strand, within GFF1948at 139.641 kb on - strand, within GFF1948at 139.871 kb on - strand, within GFF1948at 139.878 kb on + strand, within GFF1948at 139.879 kb on - strand, within GFF1948at 140.014 kb on - strand, within GFF1948at 140.080 kb on - strandat 140.080 kb on - strandat 140.110 kb on - strandat 140.244 kb on + strand, within GFF1949at 140.301 kb on - strand, within GFF1949at 140.333 kb on + strand, within GFF1949at 140.334 kb on - strand, within GFF1949at 140.381 kb on + strand, within GFF1949at 140.524 kb on - strand, within GFF1949at 140.851 kb on - strandat 141.135 kb on - strand, within GFF1950at 141.685 kb on + strand, within GFF1950at 141.686 kb on - strand, within GFF1950at 141.686 kb on - strand, within GFF1950at 141.851 kb on + strand, within GFF1950

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI
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139,228 + GFF1947 0.88 -1.1
139,340 + -1.2
139,340 + +0.2
139,341 - -0.3
139,441 + GFF1948 0.18 +1.4
139,572 + GFF1948 0.34 -0.2
139,573 - GFF1948 0.34 -1.0
139,583 - GFF1948 0.36 +0.2
139,641 - GFF1948 0.43 -0.1
139,871 - GFF1948 0.71 -0.3
139,878 + GFF1948 0.72 +1.4
139,879 - GFF1948 0.72 -0.0
140,014 - GFF1948 0.89 -0.5
140,080 - -0.6
140,080 - +0.4
140,110 - -0.2
140,244 + GFF1949 0.17 -0.0
140,301 - GFF1949 0.24 +1.1
140,333 + GFF1949 0.28 +2.4
140,334 - GFF1949 0.28 +1.5
140,381 + GFF1949 0.34 -0.5
140,524 - GFF1949 0.51 +0.4
140,851 - -1.2
141,135 - GFF1950 0.19 -1.2
141,685 + GFF1950 0.69 +1.0
141,686 - GFF1950 0.70 -1.9
141,686 - GFF1950 0.70 +0.1
141,851 + GFF1950 0.85 -0.9

Or see this region's nucleotide sequence