Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1910 and GFF1911 are separated by 56 nucleotides GFF1911 and GFF1912 are separated by 55 nucleotides GFF1912 and GFF1913 are separated by 142 nucleotides GFF1913 and GFF1914 are separated by 125 nucleotides
GFF1910 - Putative Nudix hydrolase YfcD (EC 3.6.-.-), at 104,798 to 105,352
GFF1910
GFF1911 - FIG002292: Phosphodiesterase yfcE (EC 3.1.4.-), at 105,409 to 105,960
GFF1911
GFF1912 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 106,016 to 106,660
GFF1912
GFF1913 - Probable glutathione S-transferase (EC 2.5.1.18), YfcG homolog, at 106,803 to 107,450
GFF1913
GFF1914 - Cell division inhibitor, at 107,576 to 108,469
GFF1914
Position (kb)
106
107 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 105.067 kb on + strand, within GFF1910 at 105.149 kb on + strand, within GFF1910 at 105.150 kb on - strand, within GFF1910 at 105.150 kb on - strand, within GFF1910 at 105.154 kb on + strand, within GFF1910 at 105.228 kb on + strand, within GFF1910 at 105.229 kb on - strand, within GFF1910 at 105.325 kb on + strand at 105.367 kb on - strand at 105.381 kb on + strand at 105.417 kb on + strand at 105.417 kb on + strand at 105.545 kb on + strand, within GFF1911 at 105.582 kb on + strand, within GFF1911 at 105.582 kb on + strand, within GFF1911 at 105.812 kb on + strand, within GFF1911 at 105.859 kb on + strand, within GFF1911 at 106.290 kb on - strand, within GFF1912 at 106.390 kb on + strand, within GFF1912 at 106.390 kb on + strand, within GFF1912 at 106.391 kb on - strand, within GFF1912 at 106.391 kb on - strand, within GFF1912 at 106.391 kb on - strand, within GFF1912 at 106.429 kb on - strand, within GFF1912 at 106.655 kb on - strand at 106.655 kb on - strand at 106.655 kb on - strand at 106.678 kb on + strand at 106.679 kb on + strand at 106.682 kb on + strand at 106.701 kb on + strand at 106.899 kb on - strand, within GFF1913 at 106.937 kb on + strand, within GFF1913 at 107.277 kb on + strand, within GFF1913 at 107.362 kb on - strand, within GFF1913 at 107.362 kb on - strand, within GFF1913 at 107.362 kb on - strand, within GFF1913 at 107.363 kb on + strand, within GFF1913 at 107.364 kb on - strand, within GFF1913 at 107.414 kb on - strand at 107.461 kb on + strand at 107.461 kb on + strand at 107.461 kb on + strand at 107.461 kb on + strand at 107.461 kb on + strand at 107.462 kb on - strand at 107.462 kb on - strand at 107.462 kb on - strand at 107.462 kb on - strand at 107.462 kb on - strand at 107.470 kb on + strand at 107.470 kb on + strand at 107.471 kb on - strand at 107.477 kb on + strand at 107.486 kb on - strand at 107.486 kb on - strand at 107.486 kb on - strand at 107.488 kb on - strand at 107.488 kb on - strand at 107.518 kb on - strand at 107.561 kb on + strand at 107.575 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 105,067 + GFF1910 0.48 -1.1 105,149 + GFF1910 0.63 -0.5 105,150 - GFF1910 0.63 +0.3 105,150 - GFF1910 0.63 +1.4 105,154 + GFF1910 0.64 -0.0 105,228 + GFF1910 0.77 +1.1 105,229 - GFF1910 0.78 -1.1 105,325 + +0.3 105,367 - +2.0 105,381 + +0.3 105,417 + -1.1 105,417 + -0.2 105,545 + GFF1911 0.25 +0.7 105,582 + GFF1911 0.31 -1.2 105,582 + GFF1911 0.31 +0.1 105,812 + GFF1911 0.73 +0.4 105,859 + GFF1911 0.82 -0.2 106,290 - GFF1912 0.42 -2.2 106,390 + GFF1912 0.58 +2.0 106,390 + GFF1912 0.58 -0.5 106,391 - GFF1912 0.58 -0.5 106,391 - GFF1912 0.58 -0.8 106,391 - GFF1912 0.58 -0.4 106,429 - GFF1912 0.64 -0.3 106,655 - -1.0 106,655 - -1.1 106,655 - +1.4 106,678 + +0.9 106,679 + +0.6 106,682 + -1.6 106,701 + -0.4 106,899 - GFF1913 0.15 +1.9 106,937 + GFF1913 0.21 -1.2 107,277 + GFF1913 0.73 -1.9 107,362 - GFF1913 0.86 +3.4 107,362 - GFF1913 0.86 -0.7 107,362 - GFF1913 0.86 +0.0 107,363 + GFF1913 0.86 -0.7 107,364 - GFF1913 0.87 -0.1 107,414 - -0.6 107,461 + -0.6 107,461 + +0.7 107,461 + +0.7 107,461 + -0.7 107,461 + -1.0 107,462 - -0.4 107,462 - -0.9 107,462 - -0.6 107,462 - +0.4 107,462 - +0.1 107,470 + +0.4 107,470 + +0.7 107,471 - +1.5 107,477 + -0.9 107,486 - -1.6 107,486 - -0.8 107,486 - -0.7 107,488 - +3.6 107,488 - +0.6 107,518 - -0.3 107,561 + -0.0 107,575 + -0.3
Or see this region's nucleotide sequence