Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1771 and GFF1772 overlap by 11 nucleotides GFF1772 and GFF1773 overlap by 8 nucleotides GFF1773 and GFF1774 are separated by 189 nucleotides
GFF1771 - Cytochrome c-type protein TorC, at 75,303 to 76,487
GFF1771
GFF1772 - Trimethylamine-N-oxide reductase (EC 1.6.6.9), at 76,477 to 79,029
GFF1772
GFF1773 - Chaperone protein TorD, at 79,022 to 79,654
GFF1773
GFF1774 - Cytochrome c551 peroxidase (EC 1.11.1.5), at 79,844 to 81,244
GFF1774
Position (kb)
76
77
78
79
80 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 75.484 kb on - strand, within GFF1771 at 75.485 kb on + strand, within GFF1771 at 75.486 kb on - strand, within GFF1771 at 75.558 kb on - strand, within GFF1771 at 75.558 kb on - strand, within GFF1771 at 75.578 kb on + strand, within GFF1771 at 75.579 kb on - strand, within GFF1771 at 75.702 kb on - strand, within GFF1771 at 75.732 kb on + strand, within GFF1771 at 75.732 kb on + strand, within GFF1771 at 75.732 kb on + strand, within GFF1771 at 75.733 kb on - strand, within GFF1771 at 75.733 kb on - strand, within GFF1771 at 75.733 kb on - strand, within GFF1771 at 75.909 kb on - strand, within GFF1771 at 75.927 kb on + strand, within GFF1771 at 75.928 kb on - strand, within GFF1771 at 76.110 kb on - strand, within GFF1771 at 76.241 kb on - strand, within GFF1771 at 76.241 kb on - strand, within GFF1771 at 76.251 kb on - strand, within GFF1771 at 76.282 kb on - strand, within GFF1771 at 76.283 kb on + strand, within GFF1771 at 76.404 kb on - strand at 76.404 kb on - strand at 76.891 kb on + strand, within GFF1772 at 77.023 kb on - strand, within GFF1772 at 77.023 kb on - strand, within GFF1772 at 77.169 kb on + strand, within GFF1772 at 77.224 kb on + strand, within GFF1772 at 77.228 kb on - strand, within GFF1772 at 77.323 kb on - strand, within GFF1772 at 77.334 kb on + strand, within GFF1772 at 77.335 kb on - strand, within GFF1772 at 77.385 kb on + strand, within GFF1772 at 77.431 kb on + strand, within GFF1772 at 77.431 kb on + strand, within GFF1772 at 77.431 kb on + strand, within GFF1772 at 77.461 kb on + strand, within GFF1772 at 77.600 kb on + strand, within GFF1772 at 77.601 kb on - strand, within GFF1772 at 77.601 kb on - strand, within GFF1772 at 77.601 kb on - strand, within GFF1772 at 77.607 kb on + strand, within GFF1772 at 77.607 kb on + strand, within GFF1772 at 77.608 kb on - strand, within GFF1772 at 77.966 kb on + strand, within GFF1772 at 78.007 kb on + strand, within GFF1772 at 78.105 kb on + strand, within GFF1772 at 78.118 kb on - strand, within GFF1772 at 78.334 kb on + strand, within GFF1772 at 78.334 kb on + strand, within GFF1772 at 78.366 kb on - strand, within GFF1772 at 78.366 kb on - strand, within GFF1772 at 78.552 kb on - strand, within GFF1772 at 78.552 kb on - strand, within GFF1772 at 78.552 kb on - strand, within GFF1772 at 78.620 kb on - strand, within GFF1772 at 78.648 kb on + strand, within GFF1772 at 78.846 kb on + strand at 78.851 kb on + strand at 78.852 kb on - strand at 78.854 kb on + strand at 78.871 kb on - strand at 78.871 kb on - strand at 78.904 kb on - strand at 78.928 kb on + strand at 78.940 kb on - strand at 78.994 kb on + strand at 78.994 kb on + strand at 79.027 kb on + strand at 79.056 kb on - strand at 79.065 kb on + strand at 79.066 kb on - strand at 79.068 kb on - strand at 79.068 kb on - strand at 79.068 kb on - strand at 79.119 kb on + strand, within GFF1773 at 79.120 kb on - strand, within GFF1773 at 79.916 kb on + strand at 79.928 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 75,484 - GFF1771 0.15 +0.7 75,485 + GFF1771 0.15 +0.8 75,486 - GFF1771 0.15 -0.1 75,558 - GFF1771 0.22 +1.9 75,558 - GFF1771 0.22 -0.3 75,578 + GFF1771 0.23 +0.2 75,579 - GFF1771 0.23 -0.2 75,702 - GFF1771 0.34 +0.8 75,732 + GFF1771 0.36 +0.6 75,732 + GFF1771 0.36 -0.3 75,732 + GFF1771 0.36 -0.1 75,733 - GFF1771 0.36 +0.4 75,733 - GFF1771 0.36 +0.0 75,733 - GFF1771 0.36 +0.1 75,909 - GFF1771 0.51 -0.0 75,927 + GFF1771 0.53 +1.9 75,928 - GFF1771 0.53 -0.7 76,110 - GFF1771 0.68 -0.1 76,241 - GFF1771 0.79 -0.2 76,241 - GFF1771 0.79 -0.1 76,251 - GFF1771 0.80 -0.5 76,282 - GFF1771 0.83 +0.2 76,283 + GFF1771 0.83 +0.4 76,404 - -1.0 76,404 - +0.3 76,891 + GFF1772 0.16 -0.0 77,023 - GFF1772 0.21 +0.3 77,023 - GFF1772 0.21 -0.7 77,169 + GFF1772 0.27 -0.2 77,224 + GFF1772 0.29 -0.3 77,228 - GFF1772 0.29 -1.5 77,323 - GFF1772 0.33 -0.3 77,334 + GFF1772 0.34 -0.0 77,335 - GFF1772 0.34 +1.0 77,385 + GFF1772 0.36 -0.2 77,431 + GFF1772 0.37 +0.8 77,431 + GFF1772 0.37 -0.7 77,431 + GFF1772 0.37 -0.6 77,461 + GFF1772 0.39 +0.9 77,600 + GFF1772 0.44 +0.3 77,601 - GFF1772 0.44 -0.6 77,601 - GFF1772 0.44 +0.0 77,601 - GFF1772 0.44 -1.3 77,607 + GFF1772 0.44 +0.1 77,607 + GFF1772 0.44 -0.4 77,608 - GFF1772 0.44 +0.0 77,966 + GFF1772 0.58 +0.3 78,007 + GFF1772 0.60 +0.3 78,105 + GFF1772 0.64 -0.9 78,118 - GFF1772 0.64 -0.6 78,334 + GFF1772 0.73 -0.7 78,334 + GFF1772 0.73 -0.2 78,366 - GFF1772 0.74 -1.6 78,366 - GFF1772 0.74 -0.8 78,552 - GFF1772 0.81 -0.5 78,552 - GFF1772 0.81 -0.1 78,552 - GFF1772 0.81 +0.7 78,620 - GFF1772 0.84 +0.4 78,648 + GFF1772 0.85 -0.6 78,846 + -0.3 78,851 + -1.1 78,852 - -0.7 78,854 + +0.6 78,871 - -0.5 78,871 - +0.2 78,904 - +0.3 78,928 + +0.3 78,940 - -2.4 78,994 + +0.4 78,994 + -0.0 79,027 + +0.2 79,056 - -0.4 79,065 + +1.9 79,066 - -0.6 79,068 - +0.8 79,068 - -0.7 79,068 - -0.2 79,119 + GFF1773 0.15 -0.3 79,120 - GFF1773 0.15 +0.0 79,916 + +0.5 79,928 + -1.2
Or see this region's nucleotide sequence