Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1730

Experiment: LB_plus_SM_buffer with S16_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1729 and GFF1730 are separated by 188 nucleotidesGFF1730 and GFF1731 are separated by 150 nucleotides GFF1729 - N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157), at 27,416 to 28,786 GFF1729 GFF1730 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 28,975 to 30,804 GFF1730 GFF1731 - SgrR, sugar-phosphate stress, transcriptional activator of SgrS small RNA, at 30,955 to 32,745 GFF1731 Position (kb) 28 29 30 31Strain fitness (log2 ratio) -2 -1 0 1 2at 28.846 kb on - strandat 28.949 kb on + strandat 28.949 kb on + strandat 30.802 kb on + strandat 30.803 kb on - strandat 30.803 kb on - strandat 30.806 kb on + strandat 30.806 kb on + strandat 30.806 kb on + strandat 30.807 kb on - strandat 30.814 kb on + strandat 30.815 kb on - strandat 31.005 kb on + strandat 31.006 kb on - strandat 31.324 kb on + strand, within GFF1731at 31.325 kb on - strand, within GFF1731at 31.467 kb on + strand, within GFF1731at 31.467 kb on + strand, within GFF1731at 31.622 kb on - strand, within GFF1731at 31.682 kb on - strand, within GFF1731at 31.690 kb on - strand, within GFF1731

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI
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28,846 - +1.9
28,949 + -0.3
28,949 + -0.3
30,802 + +0.5
30,803 - -0.1
30,803 - -0.7
30,806 + +0.3
30,806 + +0.3
30,806 + -0.2
30,807 - +0.4
30,814 + -0.3
30,815 - -0.7
31,005 + +0.3
31,006 - -2.5
31,324 + GFF1731 0.21 +0.5
31,325 - GFF1731 0.21 +0.7
31,467 + GFF1731 0.29 +0.2
31,467 + GFF1731 0.29 -0.2
31,622 - GFF1731 0.37 +1.1
31,682 - GFF1731 0.41 +0.3
31,690 - GFF1731 0.41 +0.2

Or see this region's nucleotide sequence