Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1666 and GFF1667 are separated by 46 nucleotides GFF1667 and GFF1668 are separated by 43 nucleotides GFF1668 and GFF1669 are separated by 20 nucleotides GFF1669 and GFF1670 are separated by 10 nucleotides GFF1670 and GFF1671 overlap by 4 nucleotides
GFF1666 - Methyl-accepting chemotaxis protein, at 51,177 to 52,838
GFF1666
GFF1667 - hypothetical protein, at 52,885 to 53,031
GFF1667
GFF1668 - Positive regulator of CheA protein activity (CheW), at 53,075 to 53,578
GFF1668
GFF1669 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 53,599 to 55,608
GFF1669
GFF1670 - Flagellar motor rotation protein MotB, at 55,619 to 56,548
GFF1670
GFF1671 - Flagellar motor rotation protein MotA, at 56,545 to 57,432
GFF1671
Position (kb) 53
54
55
56 Strain fitness (log2 ratio) -2
-1
0
1
2 at 52.776 kb on + strand at 52.839 kb on - strand at 53.030 kb on - strand at 53.274 kb on - strand, within GFF1668 at 53.402 kb on - strand, within GFF1668 at 53.429 kb on - strand, within GFF1668 at 53.513 kb on - strand, within GFF1668 at 53.581 kb on + strand at 53.581 kb on + strand at 53.582 kb on - strand at 53.620 kb on - strand at 53.724 kb on - strand at 54.123 kb on + strand, within GFF1669 at 54.325 kb on - strand, within GFF1669 at 54.325 kb on - strand, within GFF1669 at 54.573 kb on - strand, within GFF1669 at 54.580 kb on - strand, within GFF1669 at 54.588 kb on + strand, within GFF1669 at 54.613 kb on + strand, within GFF1669 at 54.614 kb on - strand, within GFF1669 at 54.634 kb on + strand, within GFF1669 at 54.722 kb on - strand, within GFF1669 at 55.111 kb on + strand, within GFF1669 at 55.112 kb on - strand, within GFF1669 at 55.184 kb on - strand, within GFF1669 at 55.263 kb on - strand, within GFF1669 at 55.327 kb on - strand, within GFF1669 at 55.599 kb on + strand at 55.600 kb on - strand at 55.683 kb on - strand at 55.683 kb on - strand at 55.692 kb on - strand at 55.751 kb on + strand, within GFF1670 at 55.768 kb on + strand, within GFF1670 at 55.768 kb on + strand, within GFF1670 at 55.839 kb on - strand, within GFF1670 at 55.922 kb on - strand, within GFF1670 at 55.988 kb on + strand, within GFF1670 at 55.989 kb on - strand, within GFF1670 at 55.989 kb on - strand, within GFF1670 at 56.032 kb on + strand, within GFF1670 at 56.278 kb on - strand, within GFF1670
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 52,776 + -0.2 52,839 - -0.3 53,030 - -1.1 53,274 - GFF1668 0.39 -1.0 53,402 - GFF1668 0.65 +0.1 53,429 - GFF1668 0.70 +0.9 53,513 - GFF1668 0.87 -0.2 53,581 + +0.7 53,581 + +2.3 53,582 - -0.3 53,620 - -0.6 53,724 - +0.0 54,123 + GFF1669 0.26 -0.6 54,325 - GFF1669 0.36 -1.4 54,325 - GFF1669 0.36 -0.6 54,573 - GFF1669 0.48 +0.4 54,580 - GFF1669 0.49 -1.1 54,588 + GFF1669 0.49 -0.1 54,613 + GFF1669 0.50 +0.4 54,614 - GFF1669 0.50 +0.2 54,634 + GFF1669 0.51 -0.7 54,722 - GFF1669 0.56 +0.9 55,111 + GFF1669 0.75 +1.8 55,112 - GFF1669 0.75 -0.6 55,184 - GFF1669 0.79 -1.6 55,263 - GFF1669 0.83 -1.6 55,327 - GFF1669 0.86 -1.1 55,599 + -2.4 55,600 - +0.1 55,683 - +0.2 55,683 - -1.3 55,692 - -0.9 55,751 + GFF1670 0.14 -1.2 55,768 + GFF1670 0.16 -1.5 55,768 + GFF1670 0.16 +0.6 55,839 - GFF1670 0.24 +0.2 55,922 - GFF1670 0.33 +0.5 55,988 + GFF1670 0.40 -0.3 55,989 - GFF1670 0.40 -0.3 55,989 - GFF1670 0.40 +0.4 56,032 + GFF1670 0.44 +0.1 56,278 - GFF1670 0.71 +1.1
Or see this region's nucleotide sequence