Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1661 and GFF1662 are separated by 193 nucleotides GFF1662 and GFF1663 are separated by 10 nucleotides GFF1663 and GFF1664 are separated by 17 nucleotides
GFF1661 - Flagellar biosynthesis protein FlhB, at 46,704 to 47,855
GFF1661
GFF1662 - Chemotaxis response - phosphatase CheZ, at 48,049 to 48,693
GFF1662
GFF1663 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 48,704 to 49,093
GFF1663
GFF1664 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 49,111 to 50,160
GFF1664
Position (kb) 48
49
50 Strain fitness (log2 ratio) -1
0
1 at 47.880 kb on - strand at 47.924 kb on - strand at 47.957 kb on + strand at 48.203 kb on - strand, within GFF1662 at 48.312 kb on + strand, within GFF1662 at 48.627 kb on + strand, within GFF1662 at 48.778 kb on - strand, within GFF1663 at 48.935 kb on - strand, within GFF1663 at 49.094 kb on - strand at 49.117 kb on - strand at 49.162 kb on + strand at 49.201 kb on - strand at 49.278 kb on - strand, within GFF1664 at 49.485 kb on - strand, within GFF1664 at 49.591 kb on - strand, within GFF1664 at 49.815 kb on + strand, within GFF1664 at 49.815 kb on + strand, within GFF1664 at 49.816 kb on - strand, within GFF1664 at 50.006 kb on - strand, within GFF1664
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 47,880 - -0.2 47,924 - -0.6 47,957 + -0.1 48,203 - GFF1662 0.24 +1.4 48,312 + GFF1662 0.41 -0.4 48,627 + GFF1662 0.90 -0.3 48,778 - GFF1663 0.19 -0.4 48,935 - GFF1663 0.59 -0.9 49,094 - +0.0 49,117 - +0.3 49,162 + -1.1 49,201 - -0.1 49,278 - GFF1664 0.16 -0.9 49,485 - GFF1664 0.36 +0.3 49,591 - GFF1664 0.46 +0.4 49,815 + GFF1664 0.67 +0.6 49,815 + GFF1664 0.67 +0.1 49,816 - GFF1664 0.67 -0.1 50,006 - GFF1664 0.85 -0.4
Or see this region's nucleotide sequence