Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1610 and GFF1611 are separated by 434 nucleotides GFF1611 and GFF1612 are separated by 54 nucleotides GFF1612 and GFF1613 are separated by 64 nucleotides GFF1613 and GFF1614 are separated by 152 nucleotides GFF1614 and GFF1615 overlap by 16 nucleotides
GFF1610 - hypothetical protein, at 346 to 498
GFF1610
GFF1611 - Phage protein, at 933 to 1,196
GFF1611
GFF1612 - FIG01046582: hypothetical protein, at 1,251 to 1,439
GFF1612
GFF1613 - lytic enzyme, at 1,504 to 1,671
GFF1613
GFF1614 - hypothetical protein, at 1,824 to 1,943
GFF1614
GFF1615 - phage-tail assembly-like protein, at 1,928 to 2,461
GFF1615
Position (kb) 1
2 Strain fitness (log2 ratio) -1
0
1
2
3 at 0.347 kb on + strand at 0.417 kb on - strand, within GFF1610 at 0.572 kb on - strand at 0.931 kb on + strand at 0.935 kb on - strand at 1.061 kb on - strand, within GFF1611 at 1.398 kb on - strand, within GFF1612 at 1.557 kb on + strand, within GFF1613 at 1.612 kb on + strand, within GFF1613 at 1.612 kb on + strand, within GFF1613 at 1.613 kb on - strand, within GFF1613 at 1.710 kb on + strand at 1.711 kb on - strand at 1.946 kb on - strand at 1.971 kb on - strand at 2.282 kb on - strand, within GFF1615
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 347 + +0.4 417 - GFF1610 0.46 -0.3 572 - +0.5 931 + +0.0 935 - -1.1 1,061 - GFF1611 0.48 +2.8 1,398 - GFF1612 0.78 -0.6 1,557 + GFF1613 0.32 -0.2 1,612 + GFF1613 0.64 +0.0 1,612 + GFF1613 0.64 -0.9 1,613 - GFF1613 0.65 +0.8 1,710 + +0.2 1,711 - -0.1 1,946 - -1.4 1,971 - -0.1 2,282 - GFF1615 0.66 +0.1
Or see this region's nucleotide sequence