Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1484 and GFF1485 are separated by 75 nucleotides GFF1485 and GFF1486 are separated by 83 nucleotides GFF1486 and GFF1487 are separated by 25 nucleotides GFF1487 and GFF1488 are separated by 209 nucleotides
GFF1484 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3), at 155,073 to 155,846
GFF1484
GFF1485 - Putative oxidoreductase component of anaerobic dehydrogenases; Chaperone protein TorD, at 155,922 to 156,515
GFF1485
GFF1486 - Putative periplasmic or exported protein, at 156,599 to 157,999
GFF1486
GFF1487 - hypothetical protein, at 158,025 to 158,138
GFF1487
GFF1488 - Putative inner membrane protein, at 158,348 to 159,208
GFF1488
Position (kb) 156
157
158 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 155.625 kb on + strand, within GFF1484 at 155.626 kb on - strand, within GFF1484 at 155.628 kb on - strand, within GFF1484 at 155.675 kb on + strand, within GFF1484 at 155.676 kb on - strand, within GFF1484 at 155.676 kb on - strand, within GFF1484 at 155.676 kb on - strand, within GFF1484 at 155.861 kb on + strand at 155.870 kb on + strand at 155.870 kb on + strand at 155.870 kb on + strand at 155.871 kb on - strand at 155.915 kb on + strand at 155.921 kb on + strand at 155.968 kb on - strand at 156.057 kb on - strand, within GFF1485 at 156.104 kb on + strand, within GFF1485 at 156.104 kb on + strand, within GFF1485 at 156.104 kb on + strand, within GFF1485 at 156.105 kb on - strand, within GFF1485 at 156.105 kb on - strand, within GFF1485 at 156.172 kb on - strand, within GFF1485 at 156.247 kb on + strand, within GFF1485 at 156.295 kb on + strand, within GFF1485 at 156.385 kb on - strand, within GFF1485 at 156.407 kb on + strand, within GFF1485 at 156.518 kb on + strand at 156.708 kb on - strand at 156.714 kb on - strand at 156.714 kb on - strand at 156.795 kb on + strand, within GFF1486 at 156.795 kb on + strand, within GFF1486 at 156.796 kb on - strand, within GFF1486 at 156.796 kb on - strand, within GFF1486 at 156.816 kb on + strand, within GFF1486 at 156.833 kb on - strand, within GFF1486 at 157.053 kb on + strand, within GFF1486 at 157.054 kb on - strand, within GFF1486 at 157.120 kb on + strand, within GFF1486 at 157.126 kb on - strand, within GFF1486 at 157.169 kb on - strand, within GFF1486 at 157.235 kb on - strand, within GFF1486 at 157.352 kb on - strand, within GFF1486 at 157.411 kb on + strand, within GFF1486 at 157.411 kb on + strand, within GFF1486 at 157.417 kb on + strand, within GFF1486 at 157.562 kb on + strand, within GFF1486 at 157.756 kb on + strand, within GFF1486 at 157.760 kb on - strand, within GFF1486 at 157.797 kb on + strand, within GFF1486 at 157.798 kb on - strand, within GFF1486 at 157.985 kb on - strand at 158.021 kb on - strand at 158.021 kb on - strand at 158.356 kb on - strand at 158.358 kb on + strand at 158.417 kb on - strand at 158.418 kb on + strand at 158.429 kb on + strand at 158.447 kb on + strand, within GFF1488 at 158.479 kb on + strand, within GFF1488 at 158.484 kb on - strand, within GFF1488 at 158.522 kb on - strand, within GFF1488 at 158.527 kb on + strand, within GFF1488 at 158.578 kb on + strand, within GFF1488 at 158.700 kb on + strand, within GFF1488 at 158.728 kb on + strand, within GFF1488 at 158.767 kb on - strand, within GFF1488 at 158.975 kb on + strand, within GFF1488
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 155,625 + GFF1484 0.71 -0.9 155,626 - GFF1484 0.71 +0.2 155,628 - GFF1484 0.72 +0.9 155,675 + GFF1484 0.78 +0.6 155,676 - GFF1484 0.78 -0.1 155,676 - GFF1484 0.78 -0.5 155,676 - GFF1484 0.78 -0.9 155,861 + -0.2 155,870 + +0.4 155,870 + +0.2 155,870 + -0.2 155,871 - +1.1 155,915 + +0.6 155,921 + +0.8 155,968 - +1.5 156,057 - GFF1485 0.23 -0.9 156,104 + GFF1485 0.31 -0.2 156,104 + GFF1485 0.31 -0.1 156,104 + GFF1485 0.31 -0.3 156,105 - GFF1485 0.31 +0.1 156,105 - GFF1485 0.31 -0.2 156,172 - GFF1485 0.42 -0.6 156,247 + GFF1485 0.55 +3.1 156,295 + GFF1485 0.63 -1.6 156,385 - GFF1485 0.78 -1.0 156,407 + GFF1485 0.82 +2.5 156,518 + +0.4 156,708 - -1.6 156,714 - +0.5 156,714 - +0.1 156,795 + GFF1486 0.14 +1.0 156,795 + GFF1486 0.14 -0.2 156,796 - GFF1486 0.14 -0.1 156,796 - GFF1486 0.14 -0.5 156,816 + GFF1486 0.15 -0.2 156,833 - GFF1486 0.17 -0.5 157,053 + GFF1486 0.32 +0.7 157,054 - GFF1486 0.32 -1.0 157,120 + GFF1486 0.37 -1.7 157,126 - GFF1486 0.38 -0.9 157,169 - GFF1486 0.41 +0.3 157,235 - GFF1486 0.45 -1.4 157,352 - GFF1486 0.54 -1.8 157,411 + GFF1486 0.58 -1.3 157,411 + GFF1486 0.58 -0.9 157,417 + GFF1486 0.58 -0.0 157,562 + GFF1486 0.69 -0.8 157,756 + GFF1486 0.83 -0.4 157,760 - GFF1486 0.83 -0.7 157,797 + GFF1486 0.86 +0.0 157,798 - GFF1486 0.86 -0.3 157,985 - -0.6 158,021 - +0.4 158,021 - +0.2 158,356 - -0.0 158,358 + -0.3 158,417 - -0.6 158,418 + -0.0 158,429 + +0.5 158,447 + GFF1488 0.11 -0.1 158,479 + GFF1488 0.15 -0.2 158,484 - GFF1488 0.16 +0.6 158,522 - GFF1488 0.20 -0.0 158,527 + GFF1488 0.21 +0.1 158,578 + GFF1488 0.27 +1.7 158,700 + GFF1488 0.41 -0.5 158,728 + GFF1488 0.44 +0.6 158,767 - GFF1488 0.49 +0.4 158,975 + GFF1488 0.73 -0.4
Or see this region's nucleotide sequence