Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1411 and GFF1412 are separated by 108 nucleotides GFF1412 and GFF1413 are separated by 178 nucleotides GFF1413 and GFF1414 overlap by 19 nucleotides GFF1414 and GFF1415 are separated by 18 nucleotides GFF1415 and GFF1416 are separated by 98 nucleotides
GFF1411 - Diacylglycerol kinase (EC 2.7.1.107), at 58,902 to 59,270
GFF1411
GFF1412 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 59,379 to 59,987
GFF1412
GFF1413 - DNA-damage-inducible protein F, at 60,166 to 61,491
GFF1413
GFF1414 - hypothetical protein, at 61,473 to 61,601
GFF1414
GFF1415 - UPF0337 protein yjbJ, at 61,620 to 61,832
GFF1415
GFF1416 - Zinc uptake regulation protein ZUR, at 61,931 to 62,446
GFF1416
Position (kb)
60
61
62 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 59.209 kb on + strand, within GFF1411 at 59.209 kb on + strand, within GFF1411 at 59.986 kb on - strand at 60.109 kb on + strand at 60.228 kb on + strand at 60.359 kb on + strand, within GFF1413 at 60.364 kb on - strand, within GFF1413 at 60.509 kb on + strand, within GFF1413 at 60.513 kb on + strand, within GFF1413 at 60.514 kb on - strand, within GFF1413 at 60.514 kb on - strand, within GFF1413 at 60.770 kb on + strand, within GFF1413 at 60.771 kb on - strand, within GFF1413 at 60.792 kb on - strand, within GFF1413 at 60.796 kb on - strand, within GFF1413 at 60.828 kb on + strand, within GFF1413 at 60.832 kb on + strand, within GFF1413 at 60.834 kb on - strand, within GFF1413 at 60.971 kb on + strand, within GFF1413 at 61.085 kb on + strand, within GFF1413 at 61.150 kb on + strand, within GFF1413 at 61.170 kb on + strand, within GFF1413 at 61.171 kb on - strand, within GFF1413 at 61.203 kb on - strand, within GFF1413 at 61.227 kb on + strand, within GFF1413 at 61.228 kb on - strand, within GFF1413 at 61.228 kb on - strand, within GFF1413 at 61.241 kb on + strand, within GFF1413 at 61.244 kb on + strand, within GFF1413 at 61.259 kb on + strand, within GFF1413 at 61.320 kb on + strand, within GFF1413 at 61.443 kb on + strand at 61.444 kb on - strand at 61.470 kb on + strand at 61.470 kb on + strand at 61.471 kb on - strand at 61.542 kb on + strand, within GFF1414 at 61.742 kb on - strand, within GFF1415 at 61.834 kb on - strand at 61.854 kb on + strand at 61.854 kb on + strand at 61.855 kb on - strand at 61.882 kb on + strand at 61.882 kb on + strand at 61.907 kb on - strand at 62.000 kb on + strand, within GFF1416 at 62.000 kb on + strand, within GFF1416 at 62.001 kb on - strand, within GFF1416 at 62.001 kb on - strand, within GFF1416 at 62.007 kb on - strand, within GFF1416 at 62.044 kb on + strand, within GFF1416 at 62.044 kb on + strand, within GFF1416 at 62.044 kb on + strand, within GFF1416 at 62.045 kb on - strand, within GFF1416 at 62.045 kb on - strand, within GFF1416 at 62.045 kb on - strand, within GFF1416 at 62.193 kb on + strand, within GFF1416 at 62.194 kb on - strand, within GFF1416 at 62.197 kb on + strand, within GFF1416 at 62.198 kb on - strand, within GFF1416 at 62.198 kb on - strand, within GFF1416 at 62.257 kb on - strand, within GFF1416 at 62.448 kb on + strand at 62.448 kb on + strand at 62.449 kb on - strand at 62.449 kb on - strand at 62.449 kb on - strand at 62.481 kb on + strand at 62.482 kb on - strand at 62.487 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 59,209 + GFF1411 0.83 +2.4 59,209 + GFF1411 0.83 -0.0 59,986 - +0.6 60,109 + +0.1 60,228 + +0.0 60,359 + GFF1413 0.15 -0.5 60,364 - GFF1413 0.15 +0.0 60,509 + GFF1413 0.26 -1.6 60,513 + GFF1413 0.26 -0.6 60,514 - GFF1413 0.26 -0.1 60,514 - GFF1413 0.26 -1.5 60,770 + GFF1413 0.46 +1.1 60,771 - GFF1413 0.46 +1.0 60,792 - GFF1413 0.47 +0.0 60,796 - GFF1413 0.48 -0.4 60,828 + GFF1413 0.50 -0.2 60,832 + GFF1413 0.50 +0.9 60,834 - GFF1413 0.50 -0.4 60,971 + GFF1413 0.61 +0.4 61,085 + GFF1413 0.69 +0.4 61,150 + GFF1413 0.74 -1.2 61,170 + GFF1413 0.76 +0.4 61,171 - GFF1413 0.76 +0.2 61,203 - GFF1413 0.78 -0.6 61,227 + GFF1413 0.80 -1.3 61,228 - GFF1413 0.80 -1.8 61,228 - GFF1413 0.80 -0.1 61,241 + GFF1413 0.81 +0.5 61,244 + GFF1413 0.81 +0.0 61,259 + GFF1413 0.82 +0.0 61,320 + GFF1413 0.87 -0.3 61,443 + +0.7 61,444 - +0.2 61,470 + -0.1 61,470 + -2.2 61,471 - +0.3 61,542 + GFF1414 0.53 -0.7 61,742 - GFF1415 0.57 +0.1 61,834 - +0.7 61,854 + +0.5 61,854 + +0.8 61,855 - -0.3 61,882 + -0.9 61,882 + -0.0 61,907 - -0.0 62,000 + GFF1416 0.13 +0.0 62,000 + GFF1416 0.13 -2.6 62,001 - GFF1416 0.14 -0.5 62,001 - GFF1416 0.14 -0.3 62,007 - GFF1416 0.15 -0.5 62,044 + GFF1416 0.22 -0.3 62,044 + GFF1416 0.22 -0.3 62,044 + GFF1416 0.22 -0.7 62,045 - GFF1416 0.22 -0.9 62,045 - GFF1416 0.22 +0.8 62,045 - GFF1416 0.22 -0.1 62,193 + GFF1416 0.51 +0.2 62,194 - GFF1416 0.51 -0.3 62,197 + GFF1416 0.52 -0.1 62,198 - GFF1416 0.52 -0.0 62,198 - GFF1416 0.52 -2.5 62,257 - GFF1416 0.63 +0.5 62,448 + -1.2 62,448 + -0.3 62,449 - +2.4 62,449 - +0.2 62,449 - -1.5 62,481 + +0.1 62,482 - +1.0 62,487 - +0.4
Or see this region's nucleotide sequence