Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1398 and GFF1399 overlap by 4 nucleotides GFF1399 and GFF1400 overlap by 1 nucleotides GFF1400 and GFF1401 are separated by 142 nucleotides GFF1401 and GFF1402 are separated by 165 nucleotides
GFF1398 - YjbF outer membrane lipoprotein, at 42,691 to 43,329
GFF1398
GFF1399 - YjbG polysaccharide synthesis-related protein, at 43,326 to 44,063
GFF1399
GFF1400 - YjbH outer membrane lipoprotein, at 44,063 to 46,159
GFF1400
GFF1401 - PsiE protein, at 46,302 to 46,712
GFF1401
GFF1402 - Maltose/maltodextrin ABC transporter, permease protein MalG, at 46,878 to 47,768
GFF1402
Position (kb)
44
45
46
47 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 43.125 kb on + strand, within GFF1398 at 43.185 kb on - strand, within GFF1398 at 43.185 kb on - strand, within GFF1398 at 43.271 kb on - strand at 43.731 kb on + strand, within GFF1399 at 43.732 kb on - strand, within GFF1399 at 43.833 kb on + strand, within GFF1399 at 43.876 kb on - strand, within GFF1399 at 43.934 kb on + strand, within GFF1399 at 43.934 kb on + strand, within GFF1399 at 43.943 kb on + strand, within GFF1399 at 43.944 kb on - strand, within GFF1399 at 44.061 kb on + strand at 44.061 kb on + strand at 44.308 kb on + strand, within GFF1400 at 44.320 kb on + strand, within GFF1400 at 44.366 kb on + strand, within GFF1400 at 44.480 kb on + strand, within GFF1400 at 44.481 kb on - strand, within GFF1400 at 44.483 kb on - strand, within GFF1400 at 44.742 kb on - strand, within GFF1400 at 44.742 kb on - strand, within GFF1400 at 44.852 kb on - strand, within GFF1400 at 44.858 kb on - strand, within GFF1400 at 44.895 kb on + strand, within GFF1400 at 45.048 kb on + strand, within GFF1400 at 45.050 kb on + strand, within GFF1400 at 45.051 kb on - strand, within GFF1400 at 45.051 kb on - strand, within GFF1400 at 45.051 kb on - strand, within GFF1400 at 45.075 kb on - strand, within GFF1400 at 45.075 kb on - strand, within GFF1400 at 45.312 kb on - strand, within GFF1400 at 45.312 kb on - strand, within GFF1400 at 45.312 kb on - strand, within GFF1400 at 45.314 kb on - strand, within GFF1400 at 45.387 kb on - strand, within GFF1400 at 45.388 kb on + strand, within GFF1400 at 45.388 kb on + strand, within GFF1400 at 45.388 kb on + strand, within GFF1400 at 45.388 kb on + strand, within GFF1400 at 45.388 kb on + strand, within GFF1400 at 45.389 kb on - strand, within GFF1400 at 45.389 kb on - strand, within GFF1400 at 45.392 kb on + strand, within GFF1400 at 45.392 kb on + strand, within GFF1400 at 45.392 kb on + strand, within GFF1400 at 45.393 kb on - strand, within GFF1400 at 45.493 kb on + strand, within GFF1400 at 45.498 kb on - strand, within GFF1400 at 45.538 kb on + strand, within GFF1400 at 45.555 kb on - strand, within GFF1400 at 45.585 kb on + strand, within GFF1400 at 45.586 kb on - strand, within GFF1400 at 45.635 kb on + strand, within GFF1400 at 45.767 kb on - strand, within GFF1400 at 45.786 kb on - strand, within GFF1400 at 45.815 kb on + strand, within GFF1400 at 45.816 kb on - strand, within GFF1400 at 45.952 kb on + strand at 46.002 kb on + strand at 46.127 kb on - strand at 46.127 kb on - strand at 46.208 kb on - strand at 46.240 kb on + strand at 46.264 kb on + strand at 46.465 kb on - strand, within GFF1401 at 46.492 kb on - strand, within GFF1401 at 46.535 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.634 kb on - strand, within GFF1401 at 46.634 kb on - strand, within GFF1401 at 46.636 kb on + strand, within GFF1401 at 46.636 kb on + strand, within GFF1401 at 46.637 kb on - strand, within GFF1401 at 46.668 kb on - strand, within GFF1401 at 46.685 kb on + strand at 46.685 kb on + strand at 46.710 kb on + strand at 46.737 kb on - strand at 46.767 kb on + strand at 46.811 kb on + strand at 46.811 kb on + strand at 46.827 kb on - strand at 46.872 kb on + strand at 47.040 kb on - strand, within GFF1402 at 47.040 kb on - strand, within GFF1402 at 47.041 kb on + strand, within GFF1402 at 47.041 kb on + strand, within GFF1402 at 47.111 kb on + strand, within GFF1402 at 47.111 kb on + strand, within GFF1402
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 43,125 + GFF1398 0.68 +0.2 43,185 - GFF1398 0.77 +0.3 43,185 - GFF1398 0.77 +0.4 43,271 - -1.4 43,731 + GFF1399 0.55 +0.9 43,732 - GFF1399 0.55 +1.3 43,833 + GFF1399 0.69 +0.1 43,876 - GFF1399 0.75 +2.1 43,934 + GFF1399 0.82 +0.2 43,934 + GFF1399 0.82 -0.9 43,943 + GFF1399 0.84 +0.0 43,944 - GFF1399 0.84 -0.5 44,061 + +0.5 44,061 + +0.3 44,308 + GFF1400 0.12 +0.7 44,320 + GFF1400 0.12 +0.8 44,366 + GFF1400 0.14 +0.8 44,480 + GFF1400 0.20 +1.2 44,481 - GFF1400 0.20 +0.8 44,483 - GFF1400 0.20 +0.7 44,742 - GFF1400 0.32 +0.9 44,742 - GFF1400 0.32 +0.6 44,852 - GFF1400 0.38 +2.7 44,858 - GFF1400 0.38 -0.1 44,895 + GFF1400 0.40 -0.4 45,048 + GFF1400 0.47 +0.3 45,050 + GFF1400 0.47 +0.5 45,051 - GFF1400 0.47 +0.0 45,051 - GFF1400 0.47 +0.3 45,051 - GFF1400 0.47 +0.2 45,075 - GFF1400 0.48 -0.1 45,075 - GFF1400 0.48 +0.4 45,312 - GFF1400 0.60 -0.2 45,312 - GFF1400 0.60 -0.9 45,312 - GFF1400 0.60 +0.4 45,314 - GFF1400 0.60 +0.4 45,387 - GFF1400 0.63 +0.2 45,388 + GFF1400 0.63 +1.4 45,388 + GFF1400 0.63 +1.6 45,388 + GFF1400 0.63 +0.1 45,388 + GFF1400 0.63 -0.7 45,388 + GFF1400 0.63 -0.1 45,389 - GFF1400 0.63 -0.5 45,389 - GFF1400 0.63 +0.3 45,392 + GFF1400 0.63 -1.1 45,392 + GFF1400 0.63 +0.6 45,392 + GFF1400 0.63 -0.4 45,393 - GFF1400 0.63 +0.2 45,493 + GFF1400 0.68 -0.2 45,498 - GFF1400 0.68 -0.5 45,538 + GFF1400 0.70 +0.5 45,555 - GFF1400 0.71 -0.1 45,585 + GFF1400 0.73 -0.1 45,586 - GFF1400 0.73 -0.3 45,635 + GFF1400 0.75 -0.3 45,767 - GFF1400 0.81 -1.2 45,786 - GFF1400 0.82 -0.1 45,815 + GFF1400 0.84 -1.9 45,816 - GFF1400 0.84 +0.1 45,952 + -0.2 46,002 + -0.2 46,127 - +2.7 46,127 - +0.1 46,208 - -0.2 46,240 + -0.6 46,264 + -0.0 46,465 - GFF1401 0.40 -1.1 46,492 - GFF1401 0.46 -1.5 46,535 + GFF1401 0.57 +0.9 46,633 + GFF1401 0.81 -0.1 46,633 + GFF1401 0.81 -2.1 46,633 + GFF1401 0.81 -0.1 46,633 + GFF1401 0.81 -0.1 46,634 - GFF1401 0.81 +0.2 46,634 - GFF1401 0.81 +0.0 46,636 + GFF1401 0.81 -1.2 46,636 + GFF1401 0.81 -0.5 46,637 - GFF1401 0.82 +0.4 46,668 - GFF1401 0.89 -1.2 46,685 + +0.3 46,685 + -0.3 46,710 + -0.7 46,737 - -0.7 46,767 + -0.2 46,811 + +0.9 46,811 + +0.0 46,827 - +0.6 46,872 + +0.3 47,040 - GFF1402 0.18 -0.2 47,040 - GFF1402 0.18 +0.9 47,041 + GFF1402 0.18 +0.0 47,041 + GFF1402 0.18 -0.6 47,111 + GFF1402 0.26 -0.9 47,111 + GFF1402 0.26 +0.2
Or see this region's nucleotide sequence