Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF132 and GFF133 overlap by 11 nucleotides GFF133 and GFF134 are separated by 30 nucleotides
GFF132 - Type IV fimbrial assembly, ATPase PilB, at 121,386 to 122,771
GFF132
GFF133 - Type IV fimbrial assembly protein PilC, at 122,761 to 123,963
GFF133
GFF134 - GMP reductase (EC 1.7.1.7), at 123,994 to 125,037
GFF134
Position (kb)
122
123
124 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 121.854 kb on - strand, within GFF132 at 121.908 kb on - strand, within GFF132 at 121.908 kb on - strand, within GFF132 at 121.983 kb on + strand, within GFF132 at 121.983 kb on + strand, within GFF132 at 121.984 kb on - strand, within GFF132 at 121.984 kb on - strand, within GFF132 at 121.984 kb on - strand, within GFF132 at 122.007 kb on - strand, within GFF132 at 122.126 kb on + strand, within GFF132 at 122.127 kb on - strand, within GFF132 at 122.254 kb on + strand, within GFF132 at 122.255 kb on - strand, within GFF132 at 122.321 kb on - strand, within GFF132 at 122.337 kb on - strand, within GFF132 at 122.362 kb on + strand, within GFF132 at 122.362 kb on + strand, within GFF132 at 122.383 kb on + strand, within GFF132 at 122.438 kb on - strand, within GFF132 at 122.438 kb on - strand, within GFF132 at 122.608 kb on + strand, within GFF132 at 122.608 kb on + strand, within GFF132 at 122.609 kb on - strand, within GFF132 at 123.462 kb on - strand, within GFF133 at 123.627 kb on + strand, within GFF133 at 123.730 kb on - strand, within GFF133 at 123.783 kb on + strand, within GFF133 at 123.795 kb on + strand, within GFF133 at 123.887 kb on + strand at 123.888 kb on - strand at 123.891 kb on + strand at 123.917 kb on - strand at 123.917 kb on - strand at 123.917 kb on - strand at 123.917 kb on - strand at 123.992 kb on + strand at 123.995 kb on + strand at 123.995 kb on + strand at 123.996 kb on - strand at 124.038 kb on + strand at 124.085 kb on + strand at 124.085 kb on + strand at 124.085 kb on + strand at 124.086 kb on - strand at 124.086 kb on - strand at 124.086 kb on - strand at 124.086 kb on - strand at 124.168 kb on - strand, within GFF134 at 124.271 kb on - strand, within GFF134 at 124.533 kb on + strand, within GFF134 at 124.656 kb on + strand, within GFF134 at 124.802 kb on + strand, within GFF134 at 124.813 kb on + strand, within GFF134 at 124.813 kb on + strand, within GFF134
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 121,854 - GFF132 0.34 +0.1 121,908 - GFF132 0.38 -0.3 121,908 - GFF132 0.38 +0.5 121,983 + GFF132 0.43 -0.3 121,983 + GFF132 0.43 +0.2 121,984 - GFF132 0.43 -1.7 121,984 - GFF132 0.43 +1.6 121,984 - GFF132 0.43 -0.5 122,007 - GFF132 0.45 +0.2 122,126 + GFF132 0.53 -0.2 122,127 - GFF132 0.53 +0.3 122,254 + GFF132 0.63 +0.0 122,255 - GFF132 0.63 +0.4 122,321 - GFF132 0.67 -0.3 122,337 - GFF132 0.69 -1.0 122,362 + GFF132 0.70 -0.3 122,362 + GFF132 0.70 -0.6 122,383 + GFF132 0.72 +1.7 122,438 - GFF132 0.76 -0.1 122,438 - GFF132 0.76 -0.9 122,608 + GFF132 0.88 -2.0 122,608 + GFF132 0.88 +0.2 122,609 - GFF132 0.88 -0.4 123,462 - GFF133 0.58 -1.1 123,627 + GFF133 0.72 -1.6 123,730 - GFF133 0.81 -1.1 123,783 + GFF133 0.85 +0.3 123,795 + GFF133 0.86 -0.7 123,887 + -0.6 123,888 - +0.5 123,891 + +0.3 123,917 - -1.3 123,917 - -1.4 123,917 - +0.5 123,917 - +0.2 123,992 + +0.2 123,995 + -0.7 123,995 + +0.9 123,996 - +0.4 124,038 + +0.1 124,085 + -0.4 124,085 + +0.0 124,085 + -0.8 124,086 - -0.7 124,086 - -1.2 124,086 - +0.4 124,086 - -0.7 124,168 - GFF134 0.17 -0.7 124,271 - GFF134 0.27 +0.4 124,533 + GFF134 0.52 +1.6 124,656 + GFF134 0.63 +1.6 124,802 + GFF134 0.77 -1.1 124,813 + GFF134 0.78 +2.4 124,813 + GFF134 0.78 +0.6
Or see this region's nucleotide sequence