Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1287 and GFF1288 overlap by 12 nucleotides GFF1288 and GFF1289 are separated by 10 nucleotides GFF1289 and GFF1290 are separated by 50 nucleotides GFF1290 and GFF1291 are separated by 62 nucleotides GFF1291 and GFF1292 overlap by 29 nucleotides
GFF1287 - putative virulence protein, at 11,786 to 13,036
GFF1287
GFF1288 - Phage minor tail protein, at 13,025 to 13,426
GFF1288
GFF1289 - Phage tail assembly, at 13,437 to 14,180
GFF1289
GFF1290 - Phage minor tail protein, at 14,231 to 14,626
GFF1290
GFF1291 - Phage minor tail protein #Phage minor tail protein T, at 14,689 to 14,961
GFF1291
GFF1292 - Phage tail length tape-measure protein 1, at 14,933 to 18,028
GFF1292
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 12.544 kb on - strand, within GFF1287 at 12.565 kb on + strand, within GFF1287 at 12.764 kb on + strand, within GFF1287 at 12.765 kb on - strand, within GFF1287 at 12.765 kb on - strand, within GFF1287 at 12.765 kb on - strand, within GFF1287 at 12.765 kb on - strand, within GFF1287 at 12.828 kb on + strand, within GFF1287 at 12.901 kb on + strand, within GFF1287 at 12.902 kb on - strand, within GFF1287 at 12.902 kb on - strand, within GFF1287 at 12.903 kb on + strand, within GFF1287 at 12.904 kb on - strand, within GFF1287 at 12.963 kb on - strand at 13.041 kb on + strand at 13.042 kb on - strand at 13.042 kb on - strand at 13.122 kb on + strand, within GFF1288 at 13.123 kb on - strand, within GFF1288 at 13.172 kb on + strand, within GFF1288 at 13.173 kb on - strand, within GFF1288 at 13.173 kb on - strand, within GFF1288 at 13.173 kb on - strand, within GFF1288 at 13.173 kb on - strand, within GFF1288 at 13.174 kb on + strand, within GFF1288 at 13.174 kb on + strand, within GFF1288 at 13.174 kb on + strand, within GFF1288 at 13.175 kb on - strand, within GFF1288 at 13.184 kb on - strand, within GFF1288 at 13.349 kb on + strand, within GFF1288 at 13.415 kb on + strand at 13.416 kb on - strand at 13.433 kb on + strand at 13.434 kb on - strand at 13.434 kb on - strand at 13.494 kb on + strand at 13.495 kb on - strand at 13.559 kb on + strand, within GFF1289 at 13.611 kb on + strand, within GFF1289 at 13.611 kb on + strand, within GFF1289 at 13.858 kb on + strand, within GFF1289 at 13.902 kb on - strand, within GFF1289 at 13.902 kb on - strand, within GFF1289 at 13.913 kb on - strand, within GFF1289 at 14.254 kb on + strand at 14.255 kb on - strand at 14.324 kb on - strand, within GFF1290 at 14.413 kb on + strand, within GFF1290 at 14.414 kb on - strand, within GFF1290 at 14.416 kb on + strand, within GFF1290 at 14.417 kb on - strand, within GFF1290 at 14.480 kb on + strand, within GFF1290 at 14.508 kb on - strand, within GFF1290 at 14.554 kb on + strand, within GFF1290 at 14.554 kb on + strand, within GFF1290 at 14.913 kb on + strand, within GFF1291 at 14.964 kb on + strand at 14.968 kb on + strand at 15.144 kb on - strand at 15.178 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 12,544 - GFF1287 0.61 -0.2 12,565 + GFF1287 0.62 +0.3 12,764 + GFF1287 0.78 -2.3 12,765 - GFF1287 0.78 +0.4 12,765 - GFF1287 0.78 +0.1 12,765 - GFF1287 0.78 +0.2 12,765 - GFF1287 0.78 +0.6 12,828 + GFF1287 0.83 +0.5 12,901 + GFF1287 0.89 -0.4 12,902 - GFF1287 0.89 -0.4 12,902 - GFF1287 0.89 -0.7 12,903 + GFF1287 0.89 -0.2 12,904 - GFF1287 0.89 -0.9 12,963 - -0.7 13,041 + -0.1 13,042 - +0.0 13,042 - +0.4 13,122 + GFF1288 0.24 -0.7 13,123 - GFF1288 0.24 -0.4 13,172 + GFF1288 0.37 +0.3 13,173 - GFF1288 0.37 -0.4 13,173 - GFF1288 0.37 -0.3 13,173 - GFF1288 0.37 -1.3 13,173 - GFF1288 0.37 -0.3 13,174 + GFF1288 0.37 +0.6 13,174 + GFF1288 0.37 -0.2 13,174 + GFF1288 0.37 -1.0 13,175 - GFF1288 0.37 +0.9 13,184 - GFF1288 0.40 +1.1 13,349 + GFF1288 0.81 +0.1 13,415 + -0.5 13,416 - -0.5 13,433 + -0.4 13,434 - +0.8 13,434 - -0.1 13,494 + +1.0 13,495 - +0.7 13,559 + GFF1289 0.16 -0.7 13,611 + GFF1289 0.23 -0.5 13,611 + GFF1289 0.23 -0.7 13,858 + GFF1289 0.57 -1.0 13,902 - GFF1289 0.62 -0.3 13,902 - GFF1289 0.62 +0.5 13,913 - GFF1289 0.64 -1.7 14,254 + +0.1 14,255 - -0.4 14,324 - GFF1290 0.23 -1.7 14,413 + GFF1290 0.46 -0.3 14,414 - GFF1290 0.46 +0.8 14,416 + GFF1290 0.47 +0.2 14,417 - GFF1290 0.47 -0.6 14,480 + GFF1290 0.63 -0.5 14,508 - GFF1290 0.70 -0.1 14,554 + GFF1290 0.82 -0.1 14,554 + GFF1290 0.82 -0.5 14,913 + GFF1291 0.82 -0.5 14,964 + -0.7 14,968 + +2.3 15,144 - -0.4 15,178 + -1.7
Or see this region's nucleotide sequence