Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1023 and GFF1024 are separated by 13 nucleotides GFF1024 and GFF1025 are separated by 375 nucleotides GFF1025 and GFF1026 overlap by 40 nucleotides GFF1026 and GFF1027 are separated by 50 nucleotides
GFF1023 - FIG023911: putative membrane protein, at 207,980 to 208,924
GFF1023
GFF1024 - FIG01045504: hypothetical protein, at 208,938 to 209,093
GFF1024
GFF1025 - Putative regulator, at 209,469 to 210,194
GFF1025
GFF1026 - Transcriptional activator protein bglJ, at 210,155 to 210,829
GFF1026
GFF1027 - tRNA proofreading protein STM4549, at 210,880 to 211,338
GFF1027
Position (kb) 209
210
211 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 208.489 kb on + strand, within GFF1023 at 208.489 kb on + strand, within GFF1023 at 208.516 kb on + strand, within GFF1023 at 208.516 kb on + strand, within GFF1023 at 208.516 kb on + strand, within GFF1023 at 208.517 kb on - strand, within GFF1023 at 208.716 kb on - strand, within GFF1023 at 208.716 kb on - strand, within GFF1023 at 208.716 kb on - strand, within GFF1023 at 208.848 kb on - strand at 208.940 kb on - strand at 208.974 kb on + strand, within GFF1024 at 209.288 kb on + strand at 209.519 kb on + strand at 209.544 kb on - strand, within GFF1025 at 209.609 kb on - strand, within GFF1025 at 209.716 kb on + strand, within GFF1025 at 210.084 kb on + strand, within GFF1025 at 210.085 kb on - strand, within GFF1025 at 210.531 kb on + strand, within GFF1026 at 210.535 kb on + strand, within GFF1026 at 210.535 kb on + strand, within GFF1026 at 210.544 kb on + strand, within GFF1026 at 210.545 kb on - strand, within GFF1026 at 210.604 kb on + strand, within GFF1026 at 210.604 kb on + strand, within GFF1026 at 210.681 kb on + strand, within GFF1026 at 210.682 kb on - strand, within GFF1026 at 210.694 kb on + strand, within GFF1026 at 210.695 kb on - strand, within GFF1026 at 210.783 kb on + strand at 210.783 kb on + strand at 210.783 kb on + strand at 210.784 kb on - strand at 210.784 kb on - strand at 210.977 kb on - strand, within GFF1027 at 210.977 kb on - strand, within GFF1027 at 210.987 kb on - strand, within GFF1027 at 211.134 kb on + strand, within GFF1027 at 211.134 kb on + strand, within GFF1027 at 211.186 kb on + strand, within GFF1027
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 208,489 + GFF1023 0.54 -0.7 208,489 + GFF1023 0.54 -0.5 208,516 + GFF1023 0.57 -2.2 208,516 + GFF1023 0.57 -0.4 208,516 + GFF1023 0.57 +0.1 208,517 - GFF1023 0.57 -0.1 208,716 - GFF1023 0.78 -0.8 208,716 - GFF1023 0.78 -0.3 208,716 - GFF1023 0.78 -0.8 208,848 - -0.4 208,940 - -0.7 208,974 + GFF1024 0.23 -0.2 209,288 + +0.1 209,519 + +0.3 209,544 - GFF1025 0.10 -0.2 209,609 - GFF1025 0.19 -0.6 209,716 + GFF1025 0.34 -0.5 210,084 + GFF1025 0.85 +0.1 210,085 - GFF1025 0.85 -0.5 210,531 + GFF1026 0.56 -0.2 210,535 + GFF1026 0.56 -0.0 210,535 + GFF1026 0.56 +2.7 210,544 + GFF1026 0.58 -0.7 210,545 - GFF1026 0.58 -2.9 210,604 + GFF1026 0.67 -0.1 210,604 + GFF1026 0.67 -0.9 210,681 + GFF1026 0.78 -1.1 210,682 - GFF1026 0.78 -0.5 210,694 + GFF1026 0.80 -0.2 210,695 - GFF1026 0.80 +2.0 210,783 + +0.4 210,783 + +0.4 210,783 + -1.0 210,784 - -0.1 210,784 - +0.5 210,977 - GFF1027 0.21 +0.5 210,977 - GFF1027 0.21 +2.0 210,987 - GFF1027 0.23 +2.2 211,134 + GFF1027 0.55 -0.9 211,134 + GFF1027 0.55 -0.2 211,186 + GFF1027 0.67 -0.2
Or see this region's nucleotide sequence