Experiment: m.b. Vancomycin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2268 and B158DRAFT_2269 are separated by 29 nucleotides B158DRAFT_2269 and B158DRAFT_2270 are separated by 191 nucleotides B158DRAFT_2270 and B158DRAFT_2271 are separated by 173 nucleotides
B158DRAFT_2268: B158DRAFT_2268 - single-stranded-DNA-specific exonuclease RecJ, at 430,051 to 431,778
_2268
B158DRAFT_2269: B158DRAFT_2269 - hypothetical protein, at 431,808 to 432,227
_2269
B158DRAFT_2270: B158DRAFT_2270 - Small-conductance mechanosensitive channel, at 432,419 to 433,258
_2270
B158DRAFT_2271: B158DRAFT_2271 - MiaB-like tRNA modifying enzyme, at 433,432 to 434,775
_2271
Position (kb)
432
433
434 Strain fitness (log2 ratio)
-2
-1
0
1 at 431.420 kb on + strand, within B158DRAFT_2268 at 431.431 kb on - strand, within B158DRAFT_2268 at 431.454 kb on + strand, within B158DRAFT_2268 at 431.454 kb on + strand, within B158DRAFT_2268 at 431.459 kb on + strand, within B158DRAFT_2268 at 431.481 kb on - strand, within B158DRAFT_2268 at 431.481 kb on - strand, within B158DRAFT_2268 at 431.483 kb on + strand, within B158DRAFT_2268 at 431.483 kb on + strand, within B158DRAFT_2268 at 431.483 kb on + strand, within B158DRAFT_2268 at 431.483 kb on + strand, within B158DRAFT_2268 at 431.491 kb on - strand, within B158DRAFT_2268 at 431.491 kb on - strand, within B158DRAFT_2268 at 431.544 kb on - strand, within B158DRAFT_2268 at 431.609 kb on + strand at 431.617 kb on - strand at 431.634 kb on + strand at 431.642 kb on - strand at 431.642 kb on - strand at 431.656 kb on + strand at 431.688 kb on + strand at 431.688 kb on + strand at 431.696 kb on - strand at 431.696 kb on - strand at 431.731 kb on + strand at 431.767 kb on + strand at 431.775 kb on - strand at 431.798 kb on + strand at 431.798 kb on + strand at 431.798 kb on + strand at 431.798 kb on + strand at 431.806 kb on - strand at 431.843 kb on + strand at 431.849 kb on - strand at 431.888 kb on + strand, within B158DRAFT_2269 at 431.901 kb on - strand, within B158DRAFT_2269 at 431.903 kb on + strand, within B158DRAFT_2269 at 431.939 kb on + strand, within B158DRAFT_2269 at 431.992 kb on - strand, within B158DRAFT_2269 at 432.032 kb on - strand, within B158DRAFT_2269 at 432.034 kb on + strand, within B158DRAFT_2269 at 432.034 kb on + strand, within B158DRAFT_2269 at 432.034 kb on + strand, within B158DRAFT_2269 at 432.039 kb on + strand, within B158DRAFT_2269 at 432.042 kb on - strand, within B158DRAFT_2269 at 432.042 kb on - strand, within B158DRAFT_2269 at 432.047 kb on - strand, within B158DRAFT_2269 at 432.047 kb on - strand, within B158DRAFT_2269 at 432.076 kb on + strand, within B158DRAFT_2269 at 432.076 kb on + strand, within B158DRAFT_2269 at 432.084 kb on - strand, within B158DRAFT_2269 at 432.084 kb on - strand, within B158DRAFT_2269 at 432.084 kb on - strand, within B158DRAFT_2269 at 432.154 kb on - strand, within B158DRAFT_2269 at 432.182 kb on + strand, within B158DRAFT_2269 at 432.182 kb on + strand, within B158DRAFT_2269 at 432.190 kb on - strand at 432.190 kb on - strand at 432.191 kb on - strand at 432.233 kb on - strand at 432.647 kb on + strand, within B158DRAFT_2270 at 432.706 kb on + strand, within B158DRAFT_2270 at 432.706 kb on + strand, within B158DRAFT_2270 at 432.742 kb on + strand, within B158DRAFT_2270 at 432.742 kb on + strand, within B158DRAFT_2270 at 432.742 kb on + strand, within B158DRAFT_2270 at 432.750 kb on - strand, within B158DRAFT_2270 at 432.750 kb on - strand, within B158DRAFT_2270 at 432.750 kb on - strand, within B158DRAFT_2270 at 432.750 kb on - strand, within B158DRAFT_2270 at 432.752 kb on + strand, within B158DRAFT_2270 at 432.791 kb on + strand, within B158DRAFT_2270 at 432.801 kb on + strand, within B158DRAFT_2270 at 432.809 kb on - strand, within B158DRAFT_2270 at 432.809 kb on - strand, within B158DRAFT_2270 at 432.809 kb on - strand, within B158DRAFT_2270 at 432.811 kb on - strand, within B158DRAFT_2270 at 432.811 kb on - strand, within B158DRAFT_2270 at 432.832 kb on + strand, within B158DRAFT_2270 at 432.832 kb on + strand, within B158DRAFT_2270 at 432.832 kb on + strand, within B158DRAFT_2270 at 432.832 kb on + strand, within B158DRAFT_2270 at 432.840 kb on - strand, within B158DRAFT_2270 at 432.840 kb on - strand, within B158DRAFT_2270 at 432.840 kb on - strand, within B158DRAFT_2270 at 432.840 kb on - strand, within B158DRAFT_2270 at 432.840 kb on - strand, within B158DRAFT_2270 at 432.840 kb on - strand, within B158DRAFT_2270 at 432.840 kb on - strand, within B158DRAFT_2270 at 432.840 kb on - strand, within B158DRAFT_2270 at 432.840 kb on - strand, within B158DRAFT_2270 at 432.848 kb on - strand, within B158DRAFT_2270 at 432.869 kb on + strand, within B158DRAFT_2270 at 432.869 kb on + strand, within B158DRAFT_2270 at 432.869 kb on + strand, within B158DRAFT_2270 at 432.877 kb on - strand, within B158DRAFT_2270 at 432.952 kb on + strand, within B158DRAFT_2270 at 432.960 kb on - strand, within B158DRAFT_2270 at 432.960 kb on - strand, within B158DRAFT_2270 at 433.096 kb on - strand, within B158DRAFT_2270 at 433.101 kb on - strand, within B158DRAFT_2270 at 433.123 kb on + strand, within B158DRAFT_2270 at 433.123 kb on + strand, within B158DRAFT_2270 at 433.123 kb on + strand, within B158DRAFT_2270 at 433.128 kb on + strand, within B158DRAFT_2270 at 433.131 kb on - strand, within B158DRAFT_2270 at 433.131 kb on - strand, within B158DRAFT_2270 at 433.131 kb on - strand, within B158DRAFT_2270 at 433.145 kb on - strand, within B158DRAFT_2270 at 433.235 kb on - strand at 433.279 kb on - strand at 433.292 kb on - strand at 433.324 kb on + strand at 433.332 kb on - strand at 433.383 kb on + strand at 433.450 kb on + strand at 433.450 kb on + strand at 433.458 kb on - strand at 433.458 kb on - strand at 433.480 kb on + strand at 433.480 kb on + strand at 433.480 kb on + strand at 433.488 kb on - strand at 433.488 kb on - strand at 433.488 kb on - strand at 433.506 kb on + strand at 433.508 kb on + strand at 433.508 kb on + strand at 433.516 kb on - strand at 433.516 kb on - strand at 433.516 kb on - strand at 433.516 kb on - strand at 433.533 kb on + strand at 433.541 kb on - strand at 433.631 kb on + strand, within B158DRAFT_2271 at 433.641 kb on - strand, within B158DRAFT_2271 at 433.695 kb on + strand, within B158DRAFT_2271 at 433.695 kb on + strand, within B158DRAFT_2271 at 433.695 kb on + strand, within B158DRAFT_2271 at 433.695 kb on + strand, within B158DRAFT_2271 at 433.695 kb on + strand, within B158DRAFT_2271 at 433.695 kb on + strand, within B158DRAFT_2271 at 433.695 kb on + strand, within B158DRAFT_2271 at 433.703 kb on - strand, within B158DRAFT_2271 at 433.703 kb on - strand, within B158DRAFT_2271 at 433.703 kb on - strand, within B158DRAFT_2271 at 433.703 kb on - strand, within B158DRAFT_2271 at 433.703 kb on - strand, within B158DRAFT_2271 at 433.703 kb on - strand, within B158DRAFT_2271 at 433.703 kb on - strand, within B158DRAFT_2271 at 433.778 kb on - strand, within B158DRAFT_2271 at 433.912 kb on + strand, within B158DRAFT_2271 at 433.946 kb on + strand, within B158DRAFT_2271 at 433.946 kb on + strand, within B158DRAFT_2271 at 434.014 kb on + strand, within B158DRAFT_2271 at 434.212 kb on - strand, within B158DRAFT_2271 at 434.212 kb on - strand, within B158DRAFT_2271 at 434.256 kb on + strand, within B158DRAFT_2271 at 434.256 kb on + strand, within B158DRAFT_2271
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Vancomycin 0.125 mg/ml remove 431,420 + B158DRAFT_2268 0.79 -1.7 431,431 - B158DRAFT_2268 0.80 -1.2 431,454 + B158DRAFT_2268 0.81 -1.8 431,454 + B158DRAFT_2268 0.81 +0.2 431,459 + B158DRAFT_2268 0.81 -1.7 431,481 - B158DRAFT_2268 0.83 -1.8 431,481 - B158DRAFT_2268 0.83 -1.1 431,483 + B158DRAFT_2268 0.83 -1.1 431,483 + B158DRAFT_2268 0.83 -0.8 431,483 + B158DRAFT_2268 0.83 -1.8 431,483 + B158DRAFT_2268 0.83 -1.6 431,491 - B158DRAFT_2268 0.83 -1.2 431,491 - B158DRAFT_2268 0.83 -1.2 431,544 - B158DRAFT_2268 0.86 -1.4 431,609 + -1.2 431,617 - -0.9 431,634 + -0.8 431,642 - -2.4 431,642 - -1.0 431,656 + -0.4 431,688 + -1.2 431,688 + -1.1 431,696 - -1.0 431,696 - -0.9 431,731 + -1.8 431,767 + -0.4 431,775 - +0.1 431,798 + +0.1 431,798 + -0.4 431,798 + -0.1 431,798 + -0.3 431,806 - +0.1 431,843 + -0.4 431,849 - -0.3 431,888 + B158DRAFT_2269 0.19 +0.2 431,901 - B158DRAFT_2269 0.22 -0.5 431,903 + B158DRAFT_2269 0.23 +0.3 431,939 + B158DRAFT_2269 0.31 +0.1 431,992 - B158DRAFT_2269 0.44 +0.1 432,032 - B158DRAFT_2269 0.53 +0.4 432,034 + B158DRAFT_2269 0.54 -0.1 432,034 + B158DRAFT_2269 0.54 -0.5 432,034 + B158DRAFT_2269 0.54 -0.1 432,039 + B158DRAFT_2269 0.55 -0.0 432,042 - B158DRAFT_2269 0.56 -0.6 432,042 - B158DRAFT_2269 0.56 -0.3 432,047 - B158DRAFT_2269 0.57 -0.0 432,047 - B158DRAFT_2269 0.57 -0.3 432,076 + B158DRAFT_2269 0.64 +0.2 432,076 + B158DRAFT_2269 0.64 -0.2 432,084 - B158DRAFT_2269 0.66 -0.3 432,084 - B158DRAFT_2269 0.66 +0.3 432,084 - B158DRAFT_2269 0.66 -0.2 432,154 - B158DRAFT_2269 0.82 -0.3 432,182 + B158DRAFT_2269 0.89 -0.1 432,182 + B158DRAFT_2269 0.89 -0.4 432,190 - +0.5 432,190 - +1.3 432,191 - -0.0 432,233 - +0.3 432,647 + B158DRAFT_2270 0.27 -0.4 432,706 + B158DRAFT_2270 0.34 +0.1 432,706 + B158DRAFT_2270 0.34 -1.0 432,742 + B158DRAFT_2270 0.38 -0.0 432,742 + B158DRAFT_2270 0.38 -0.0 432,742 + B158DRAFT_2270 0.38 +0.5 432,750 - B158DRAFT_2270 0.39 -0.3 432,750 - B158DRAFT_2270 0.39 -0.1 432,750 - B158DRAFT_2270 0.39 +0.2 432,750 - B158DRAFT_2270 0.39 -0.4 432,752 + B158DRAFT_2270 0.40 -0.3 432,791 + B158DRAFT_2270 0.44 -0.4 432,801 + B158DRAFT_2270 0.45 +0.2 432,809 - B158DRAFT_2270 0.46 -0.3 432,809 - B158DRAFT_2270 0.46 -0.0 432,809 - B158DRAFT_2270 0.46 -0.4 432,811 - B158DRAFT_2270 0.47 -0.3 432,811 - B158DRAFT_2270 0.47 -1.1 432,832 + B158DRAFT_2270 0.49 -0.3 432,832 + B158DRAFT_2270 0.49 -0.3 432,832 + B158DRAFT_2270 0.49 -0.4 432,832 + B158DRAFT_2270 0.49 -0.2 432,840 - B158DRAFT_2270 0.50 +0.1 432,840 - B158DRAFT_2270 0.50 -0.2 432,840 - B158DRAFT_2270 0.50 +0.1 432,840 - B158DRAFT_2270 0.50 +0.2 432,840 - B158DRAFT_2270 0.50 -0.1 432,840 - B158DRAFT_2270 0.50 -0.2 432,840 - B158DRAFT_2270 0.50 -0.1 432,840 - B158DRAFT_2270 0.50 -0.2 432,840 - B158DRAFT_2270 0.50 +0.2 432,848 - B158DRAFT_2270 0.51 -0.5 432,869 + B158DRAFT_2270 0.54 -0.3 432,869 + B158DRAFT_2270 0.54 -0.3 432,869 + B158DRAFT_2270 0.54 -0.2 432,877 - B158DRAFT_2270 0.55 -0.2 432,952 + B158DRAFT_2270 0.63 -0.3 432,960 - B158DRAFT_2270 0.64 -0.4 432,960 - B158DRAFT_2270 0.64 -0.1 433,096 - B158DRAFT_2270 0.81 -0.2 433,101 - B158DRAFT_2270 0.81 +0.1 433,123 + B158DRAFT_2270 0.84 +0.5 433,123 + B158DRAFT_2270 0.84 +0.6 433,123 + B158DRAFT_2270 0.84 -0.0 433,128 + B158DRAFT_2270 0.84 +0.3 433,131 - B158DRAFT_2270 0.85 -1.0 433,131 - B158DRAFT_2270 0.85 -0.4 433,131 - B158DRAFT_2270 0.85 -0.2 433,145 - B158DRAFT_2270 0.86 -0.2 433,235 - -0.2 433,279 - +0.1 433,292 - -0.1 433,324 + -0.5 433,332 - -0.4 433,383 + -0.0 433,450 + -0.2 433,450 + -0.5 433,458 - -0.4 433,458 - -0.7 433,480 + -0.9 433,480 + +0.1 433,480 + -0.0 433,488 - +0.1 433,488 - -0.1 433,488 - -0.2 433,506 + +0.1 433,508 + -0.8 433,508 + -0.6 433,516 - -0.1 433,516 - -0.5 433,516 - -0.2 433,516 - -0.4 433,533 + -0.6 433,541 - -0.2 433,631 + B158DRAFT_2271 0.15 -0.1 433,641 - B158DRAFT_2271 0.16 -0.4 433,695 + B158DRAFT_2271 0.20 -0.9 433,695 + B158DRAFT_2271 0.20 -1.1 433,695 + B158DRAFT_2271 0.20 -0.4 433,695 + B158DRAFT_2271 0.20 -0.7 433,695 + B158DRAFT_2271 0.20 -0.1 433,695 + B158DRAFT_2271 0.20 -0.7 433,695 + B158DRAFT_2271 0.20 -0.7 433,703 - B158DRAFT_2271 0.20 -0.6 433,703 - B158DRAFT_2271 0.20 -0.5 433,703 - B158DRAFT_2271 0.20 -0.6 433,703 - B158DRAFT_2271 0.20 -0.2 433,703 - B158DRAFT_2271 0.20 -0.7 433,703 - B158DRAFT_2271 0.20 -0.9 433,703 - B158DRAFT_2271 0.20 -0.1 433,778 - B158DRAFT_2271 0.26 -0.1 433,912 + B158DRAFT_2271 0.36 -0.3 433,946 + B158DRAFT_2271 0.38 +0.0 433,946 + B158DRAFT_2271 0.38 -0.5 434,014 + B158DRAFT_2271 0.43 -0.4 434,212 - B158DRAFT_2271 0.58 -0.7 434,212 - B158DRAFT_2271 0.58 -1.0 434,256 + B158DRAFT_2271 0.61 -0.8 434,256 + B158DRAFT_2271 0.61 -0.3
Or see this region's nucleotide sequence