Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF985 and GFF986 are separated by 12 nucleotides GFF986 and GFF987 overlap by 4 nucleotides GFF987 and GFF988 are separated by 872 nucleotides
GFF985 - Isoaspartyl dipeptidase (EC 3.4.19.5) @ Asp-X dipeptidase, at 169,445 to 170,617
GFF985
GFF986 - Putative membrane protein, at 170,630 to 171,091
GFF986
GFF987 - FIG00637915: hypothetical protein, at 171,088 to 171,759
GFF987
GFF988 - Putative transport protein, at 172,632 to 173,759
GFF988
Position (kb)
171
172 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 170.531 kb on + strand at 170.532 kb on - strand at 170.690 kb on - strand, within GFF986 at 170.700 kb on + strand, within GFF986 at 170.770 kb on - strand, within GFF986 at 170.783 kb on + strand, within GFF986 at 170.783 kb on + strand, within GFF986 at 170.792 kb on - strand, within GFF986 at 170.857 kb on + strand, within GFF986 at 170.858 kb on - strand, within GFF986 at 170.977 kb on + strand, within GFF986 at 171.058 kb on + strand at 171.058 kb on + strand at 171.058 kb on + strand at 171.059 kb on - strand at 171.073 kb on + strand at 171.074 kb on - strand at 171.074 kb on - strand at 171.074 kb on - strand at 171.077 kb on + strand at 171.078 kb on - strand at 171.078 kb on - strand at 171.255 kb on + strand, within GFF987 at 171.796 kb on + strand at 171.797 kb on - strand at 171.864 kb on + strand at 171.865 kb on - strand at 171.865 kb on - strand at 171.952 kb on + strand at 172.349 kb on + strand at 172.349 kb on + strand at 172.454 kb on - strand at 172.454 kb on - strand at 172.588 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 170,531 + -2.0 170,532 - +0.8 170,690 - GFF986 0.13 -0.0 170,700 + GFF986 0.15 -0.1 170,770 - GFF986 0.30 +0.7 170,783 + GFF986 0.33 -1.8 170,783 + GFF986 0.33 +1.2 170,792 - GFF986 0.35 -0.3 170,857 + GFF986 0.49 +1.0 170,858 - GFF986 0.49 +1.6 170,977 + GFF986 0.75 +1.3 171,058 + -0.6 171,058 + -0.3 171,058 + -1.5 171,059 - -0.9 171,073 + +0.2 171,074 - +0.4 171,074 - -0.3 171,074 - -1.5 171,077 + -3.5 171,078 - +1.2 171,078 - -1.5 171,255 + GFF987 0.25 -0.8 171,796 + -1.5 171,797 - -1.2 171,864 + -0.3 171,865 - +0.1 171,865 - -2.2 171,952 + +3.1 172,349 + -1.0 172,349 + -0.6 172,454 - +1.4 172,454 - -0.3 172,588 + +0.5
Or see this region's nucleotide sequence