Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF953 and GFF954 are separated by 61 nucleotides GFF954 and GFF955 are separated by 24 nucleotides GFF955 and GFF956 are separated by 216 nucleotides GFF956 and GFF957 are separated by 27 nucleotides
GFF953 - L-idonate, D-gluconate, 5-keto-D-gluconate transporter, at 129,145 to 130,464
GFF953
GFF954 - 5-keto-D-gluconate 5-reductase (EC 1.1.1.69), at 130,526 to 131,290
GFF954
GFF955 - L-idonate 5-dehydrogenase (EC 1.1.1.264), at 131,315 to 132,346
GFF955
GFF956 - Gluconokinase (EC 2.7.1.12), at 132,563 to 133,093
GFF956
GFF957 - Alcohol dehydrogenase (EC 1.1.1.1), at 133,121 to 134,140
GFF957
Position (kb) 131
132
133 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 130.478 kb on - strand at 130.753 kb on + strand, within GFF954 at 130.897 kb on + strand, within GFF954 at 131.053 kb on + strand, within GFF954 at 131.096 kb on - strand, within GFF954 at 131.117 kb on + strand, within GFF954 at 131.182 kb on + strand, within GFF954 at 131.224 kb on - strand at 131.293 kb on + strand at 131.471 kb on - strand, within GFF955 at 131.511 kb on - strand, within GFF955 at 131.754 kb on - strand, within GFF955 at 131.788 kb on + strand, within GFF955 at 131.824 kb on + strand, within GFF955 at 132.406 kb on - strand at 132.417 kb on - strand at 132.844 kb on + strand, within GFF956 at 132.862 kb on - strand, within GFF956 at 132.888 kb on - strand, within GFF956 at 132.896 kb on - strand, within GFF956 at 132.932 kb on - strand, within GFF956 at 133.016 kb on - strand, within GFF956 at 133.059 kb on + strand at 133.136 kb on + strand at 133.269 kb on + strand, within GFF957 at 133.269 kb on + strand, within GFF957
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 130,478 - -2.5 130,753 + GFF954 0.30 +1.2 130,897 + GFF954 0.48 +0.6 131,053 + GFF954 0.69 -2.2 131,096 - GFF954 0.75 +1.5 131,117 + GFF954 0.77 +0.1 131,182 + GFF954 0.86 -0.2 131,224 - -1.2 131,293 + +1.3 131,471 - GFF955 0.15 +3.3 131,511 - GFF955 0.19 -1.2 131,754 - GFF955 0.43 -2.1 131,788 + GFF955 0.46 -0.5 131,824 + GFF955 0.49 +1.4 132,406 - -0.2 132,417 - -1.2 132,844 + GFF956 0.53 +0.5 132,862 - GFF956 0.56 -1.2 132,888 - GFF956 0.61 +0.6 132,896 - GFF956 0.63 -0.2 132,932 - GFF956 0.69 -1.0 133,016 - GFF956 0.85 -1.0 133,059 + -0.2 133,136 + +0.3 133,269 + GFF957 0.15 +0.3 133,269 + GFF957 0.15 +0.7
Or see this region's nucleotide sequence