Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF81 and GFF82 are separated by 91 nucleotides GFF82 and GFF83 are separated by 138 nucleotides GFF83 and GFF84 are separated by 140 nucleotides
GFF81 - Ferric hydroxamate outer membrane receptor FhuA, at 63,162 to 65,273
GFF81
GFF82 - hypothetical protein, at 65,365 to 65,505
GFF82
GFF83 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 65,644 to 68,166
GFF83
GFF84 - ATP-dependent helicase HrpB, at 68,307 to 70,781
GFF84
Position (kb) 65
66
67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 64.782 kb on - strand, within GFF81 at 64.997 kb on + strand, within GFF81 at 65.026 kb on - strand, within GFF81 at 65.132 kb on + strand at 65.133 kb on - strand at 65.296 kb on - strand at 65.355 kb on + strand at 65.356 kb on - strand at 65.357 kb on + strand at 65.396 kb on - strand, within GFF82 at 65.528 kb on + strand at 65.572 kb on + strand at 65.809 kb on - strand at 65.874 kb on - strand at 65.917 kb on + strand, within GFF83 at 65.982 kb on - strand, within GFF83 at 66.179 kb on + strand, within GFF83 at 66.228 kb on - strand, within GFF83 at 66.238 kb on - strand, within GFF83 at 66.350 kb on - strand, within GFF83 at 66.512 kb on + strand, within GFF83 at 66.602 kb on + strand, within GFF83 at 66.603 kb on - strand, within GFF83 at 66.604 kb on + strand, within GFF83 at 66.619 kb on + strand, within GFF83 at 66.632 kb on + strand, within GFF83 at 66.699 kb on - strand, within GFF83 at 66.742 kb on - strand, within GFF83 at 66.891 kb on + strand, within GFF83 at 66.891 kb on + strand, within GFF83 at 66.940 kb on - strand, within GFF83 at 67.011 kb on - strand, within GFF83 at 67.042 kb on + strand, within GFF83 at 67.051 kb on + strand, within GFF83 at 67.104 kb on - strand, within GFF83 at 67.181 kb on + strand, within GFF83 at 67.182 kb on - strand, within GFF83 at 67.182 kb on - strand, within GFF83 at 67.331 kb on + strand, within GFF83 at 67.440 kb on + strand, within GFF83 at 67.500 kb on + strand, within GFF83 at 67.620 kb on + strand, within GFF83 at 67.731 kb on + strand, within GFF83 at 67.731 kb on + strand, within GFF83 at 67.732 kb on - strand, within GFF83 at 67.732 kb on - strand, within GFF83 at 67.732 kb on - strand, within GFF83 at 67.862 kb on + strand, within GFF83 at 67.863 kb on - strand, within GFF83 at 67.979 kb on - strand at 68.011 kb on - strand at 68.026 kb on - strand at 68.057 kb on + strand at 68.058 kb on - strand at 68.134 kb on - strand at 68.134 kb on - strand at 68.278 kb on + strand at 68.278 kb on + strand at 68.298 kb on + strand at 68.298 kb on + strand at 68.299 kb on - strand at 68.301 kb on - strand at 68.314 kb on + strand at 68.659 kb on + strand, within GFF84 at 68.721 kb on + strand, within GFF84 at 68.721 kb on + strand, within GFF84 at 68.722 kb on - strand, within GFF84 at 68.722 kb on - strand, within GFF84 at 69.117 kb on - strand, within GFF84
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 64,782 - GFF81 0.77 -0.3 64,997 + GFF81 0.87 -0.4 65,026 - GFF81 0.88 +0.8 65,132 + +0.4 65,133 - +0.9 65,296 - +0.2 65,355 + +0.1 65,356 - +1.3 65,357 + +0.6 65,396 - GFF82 0.22 -0.0 65,528 + -0.9 65,572 + +0.4 65,809 - -0.6 65,874 - +1.0 65,917 + GFF83 0.11 +0.2 65,982 - GFF83 0.13 -3.2 66,179 + GFF83 0.21 +0.6 66,228 - GFF83 0.23 +1.1 66,238 - GFF83 0.24 +0.1 66,350 - GFF83 0.28 -1.1 66,512 + GFF83 0.34 -0.9 66,602 + GFF83 0.38 -0.9 66,603 - GFF83 0.38 +0.3 66,604 + GFF83 0.38 -0.8 66,619 + GFF83 0.39 -0.1 66,632 + GFF83 0.39 +0.5 66,699 - GFF83 0.42 -0.3 66,742 - GFF83 0.44 +0.2 66,891 + GFF83 0.49 -1.0 66,891 + GFF83 0.49 +0.4 66,940 - GFF83 0.51 -0.9 67,011 - GFF83 0.54 +2.4 67,042 + GFF83 0.55 -1.2 67,051 + GFF83 0.56 -0.4 67,104 - GFF83 0.58 -0.5 67,181 + GFF83 0.61 -0.7 67,182 - GFF83 0.61 -0.6 67,182 - GFF83 0.61 -2.6 67,331 + GFF83 0.67 -1.7 67,440 + GFF83 0.71 +0.6 67,500 + GFF83 0.74 +0.6 67,620 + GFF83 0.78 -1.3 67,731 + GFF83 0.83 +1.0 67,731 + GFF83 0.83 +0.8 67,732 - GFF83 0.83 +1.2 67,732 - GFF83 0.83 -1.7 67,732 - GFF83 0.83 +0.3 67,862 + GFF83 0.88 +0.9 67,863 - GFF83 0.88 +0.6 67,979 - -0.0 68,011 - -0.2 68,026 - +0.5 68,057 + -0.7 68,058 - -0.3 68,134 - -0.1 68,134 - -0.3 68,278 + -0.1 68,278 + -0.2 68,298 + +0.4 68,298 + -0.1 68,299 - +0.5 68,301 - +0.6 68,314 + +0.9 68,659 + GFF84 0.14 -2.2 68,721 + GFF84 0.17 +0.2 68,721 + GFF84 0.17 -1.4 68,722 - GFF84 0.17 -0.3 68,722 - GFF84 0.17 -0.7 69,117 - GFF84 0.33 +0.5
Or see this region's nucleotide sequence