Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF725 and GFF726 are separated by 202 nucleotides GFF726 and GFF727 are separated by 10 nucleotides
GFF725 - Molybdopterin biosynthesis protein MoeA, at 82,775 to 84,010
GFF725
GFF726 - Isoaspartyl aminopeptidase (EC 3.4.19.5) @ Asp-X dipeptidase, at 84,213 to 85,154
GFF726
GFF727 - Dipeptide transport ATP-binding protein DppD in protein degradation cluster, at 85,165 to 87,036
GFF727
Position (kb) 84
85
86 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 83.232 kb on + strand, within GFF725 at 83.232 kb on + strand, within GFF725 at 83.267 kb on + strand, within GFF725 at 83.267 kb on + strand, within GFF725 at 83.268 kb on - strand, within GFF725 at 83.336 kb on - strand, within GFF725 at 83.386 kb on + strand, within GFF725 at 83.387 kb on - strand, within GFF725 at 83.387 kb on - strand, within GFF725 at 83.482 kb on + strand, within GFF725 at 83.540 kb on - strand, within GFF725 at 83.950 kb on + strand at 84.017 kb on - strand at 84.017 kb on - strand at 84.045 kb on + strand at 84.048 kb on + strand at 84.049 kb on - strand at 84.049 kb on - strand at 84.049 kb on - strand at 84.073 kb on + strand at 84.110 kb on - strand at 84.114 kb on - strand at 84.119 kb on - strand at 84.131 kb on - strand at 84.133 kb on - strand at 84.174 kb on + strand at 84.174 kb on + strand at 84.289 kb on + strand at 84.290 kb on - strand at 84.295 kb on - strand at 84.367 kb on + strand, within GFF726 at 84.367 kb on + strand, within GFF726 at 84.432 kb on + strand, within GFF726 at 84.433 kb on - strand, within GFF726 at 84.571 kb on + strand, within GFF726 at 84.572 kb on - strand, within GFF726 at 84.819 kb on - strand, within GFF726 at 84.819 kb on - strand, within GFF726 at 85.065 kb on - strand at 85.098 kb on + strand at 85.098 kb on + strand at 85.099 kb on - strand at 85.524 kb on - strand, within GFF727 at 85.527 kb on + strand, within GFF727 at 85.891 kb on + strand, within GFF727 at 85.923 kb on - strand, within GFF727 at 85.956 kb on + strand, within GFF727 at 86.100 kb on + strand, within GFF727 at 86.100 kb on + strand, within GFF727 at 86.100 kb on + strand, within GFF727
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 83,232 + GFF725 0.37 -0.1 83,232 + GFF725 0.37 -0.4 83,267 + GFF725 0.40 -0.4 83,267 + GFF725 0.40 -0.8 83,268 - GFF725 0.40 +2.0 83,336 - GFF725 0.45 -0.8 83,386 + GFF725 0.49 +0.2 83,387 - GFF725 0.50 -1.7 83,387 - GFF725 0.50 +0.3 83,482 + GFF725 0.57 +0.0 83,540 - GFF725 0.62 -1.6 83,950 + +0.4 84,017 - +0.1 84,017 - -0.6 84,045 + -1.1 84,048 + +0.5 84,049 - +0.2 84,049 - -1.4 84,049 - +1.1 84,073 + -1.1 84,110 - -0.2 84,114 - -0.6 84,119 - +0.3 84,131 - +0.2 84,133 - -1.6 84,174 + -2.5 84,174 + +1.0 84,289 + -0.5 84,290 - -1.8 84,295 - -2.6 84,367 + GFF726 0.16 -4.0 84,367 + GFF726 0.16 +0.7 84,432 + GFF726 0.23 +0.7 84,433 - GFF726 0.23 -0.5 84,571 + GFF726 0.38 -1.5 84,572 - GFF726 0.38 -0.6 84,819 - GFF726 0.64 -0.8 84,819 - GFF726 0.64 -1.1 85,065 - +0.5 85,098 + +0.2 85,098 + +0.1 85,099 - -0.9 85,524 - GFF727 0.19 -2.2 85,527 + GFF727 0.19 -2.9 85,891 + GFF727 0.39 -0.8 85,923 - GFF727 0.40 +1.2 85,956 + GFF727 0.42 +0.7 86,100 + GFF727 0.50 -0.8 86,100 + GFF727 0.50 -1.1 86,100 + GFF727 0.50 -1.8
Or see this region's nucleotide sequence