Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF709 and GFF710 are separated by 353 nucleotides GFF710 and GFF711 are separated by 62 nucleotides
GFF709 - Putative DMT superfamily metabolite efflux protein precursor, at 65,357 to 66,244
GFF709
GFF710 - Outer membrane protein X precursor, at 66,598 to 67,113
GFF710
GFF711 - FIG00732228: membrane protein, at 67,176 to 68,756
GFF711
Position (kb) 66
67
68 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 65.757 kb on + strand, within GFF709 at 65.821 kb on + strand, within GFF709 at 65.821 kb on + strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.878 kb on + strand, within GFF709 at 65.979 kb on + strand, within GFF709 at 65.980 kb on - strand, within GFF709 at 65.984 kb on + strand, within GFF709 at 65.984 kb on + strand, within GFF709 at 65.984 kb on + strand, within GFF709 at 65.985 kb on - strand, within GFF709 at 65.988 kb on + strand, within GFF709 at 66.015 kb on + strand, within GFF709 at 66.015 kb on + strand, within GFF709 at 66.015 kb on + strand, within GFF709 at 66.016 kb on - strand, within GFF709 at 66.226 kb on + strand at 66.395 kb on - strand at 66.429 kb on - strand at 66.439 kb on + strand at 66.495 kb on + strand at 66.573 kb on + strand at 66.573 kb on + strand at 66.662 kb on + strand, within GFF710 at 66.675 kb on + strand, within GFF710 at 66.675 kb on + strand, within GFF710 at 66.748 kb on + strand, within GFF710 at 66.749 kb on - strand, within GFF710 at 66.836 kb on - strand, within GFF710 at 66.904 kb on + strand, within GFF710 at 66.979 kb on + strand, within GFF710 at 66.992 kb on - strand, within GFF710 at 67.065 kb on + strand at 67.111 kb on + strand at 67.247 kb on - strand at 67.308 kb on - strand at 67.364 kb on + strand, within GFF711 at 67.382 kb on + strand, within GFF711 at 67.501 kb on + strand, within GFF711 at 67.501 kb on + strand, within GFF711 at 67.582 kb on + strand, within GFF711 at 67.624 kb on + strand, within GFF711 at 67.624 kb on + strand, within GFF711 at 67.625 kb on - strand, within GFF711 at 67.637 kb on + strand, within GFF711 at 67.916 kb on - strand, within GFF711 at 67.990 kb on + strand, within GFF711 at 68.101 kb on + strand, within GFF711 at 68.101 kb on + strand, within GFF711 at 68.102 kb on - strand, within GFF711
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 65,757 + GFF709 0.45 -1.5 65,821 + GFF709 0.52 +1.3 65,821 + GFF709 0.52 -0.8 65,822 - GFF709 0.52 -0.9 65,822 - GFF709 0.52 +0.5 65,822 - GFF709 0.52 -0.1 65,822 - GFF709 0.52 +0.1 65,822 - GFF709 0.52 -0.3 65,878 + GFF709 0.59 -0.8 65,979 + GFF709 0.70 -0.0 65,980 - GFF709 0.70 +1.2 65,984 + GFF709 0.71 +0.4 65,984 + GFF709 0.71 -0.6 65,984 + GFF709 0.71 +0.1 65,985 - GFF709 0.71 -0.7 65,988 + GFF709 0.71 -2.1 66,015 + GFF709 0.74 +0.8 66,015 + GFF709 0.74 -0.8 66,015 + GFF709 0.74 +0.4 66,016 - GFF709 0.74 +1.6 66,226 + -1.6 66,395 - +1.0 66,429 - -2.7 66,439 + +0.8 66,495 + -0.1 66,573 + +0.0 66,573 + +0.3 66,662 + GFF710 0.12 -0.4 66,675 + GFF710 0.15 -1.7 66,675 + GFF710 0.15 +1.0 66,748 + GFF710 0.29 -3.0 66,749 - GFF710 0.29 +1.5 66,836 - GFF710 0.46 -2.2 66,904 + GFF710 0.59 -2.4 66,979 + GFF710 0.74 -0.5 66,992 - GFF710 0.76 -1.9 67,065 + -1.1 67,111 + +0.2 67,247 - -1.1 67,308 - -1.9 67,364 + GFF711 0.12 +0.2 67,382 + GFF711 0.13 -0.1 67,501 + GFF711 0.21 -1.1 67,501 + GFF711 0.21 +1.3 67,582 + GFF711 0.26 -1.8 67,624 + GFF711 0.28 -0.6 67,624 + GFF711 0.28 +0.8 67,625 - GFF711 0.28 -0.5 67,637 + GFF711 0.29 -2.7 67,916 - GFF711 0.47 +1.0 67,990 + GFF711 0.51 -2.4 68,101 + GFF711 0.59 +0.4 68,101 + GFF711 0.59 -1.3 68,102 - GFF711 0.59 -2.6
Or see this region's nucleotide sequence