Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF668 and GFF669 are separated by 84 nucleotides GFF669 and GFF670 are separated by 57 nucleotides GFF670 and GFF671 are separated by 86 nucleotides
GFF668 - Histidine ammonia-lyase (EC 4.3.1.3), at 23,719 to 25,239
GFF668
GFF669 - UPF0098 protein ybhB, at 25,324 to 25,800
GFF669
GFF670 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62), at 25,858 to 27,147
GFF670
GFF671 - Biotin synthase (EC 2.8.1.6), at 27,234 to 28,274
GFF671
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 25.146 kb on + strand at 25.147 kb on - strand at 25.233 kb on + strand at 25.233 kb on + strand at 25.234 kb on - strand at 25.234 kb on - strand at 25.446 kb on + strand, within GFF669 at 25.452 kb on + strand, within GFF669 at 25.455 kb on - strand, within GFF669 at 25.455 kb on - strand, within GFF669 at 25.714 kb on - strand, within GFF669 at 25.792 kb on - strand at 25.850 kb on - strand at 25.960 kb on + strand at 25.961 kb on - strand at 26.218 kb on - strand, within GFF670 at 26.235 kb on + strand, within GFF670 at 26.236 kb on - strand, within GFF670 at 26.408 kb on - strand, within GFF670 at 26.451 kb on + strand, within GFF670 at 26.452 kb on - strand, within GFF670 at 26.452 kb on - strand, within GFF670 at 26.458 kb on - strand, within GFF670 at 26.480 kb on + strand, within GFF670 at 26.480 kb on + strand, within GFF670 at 26.482 kb on + strand, within GFF670 at 26.482 kb on + strand, within GFF670 at 26.482 kb on + strand, within GFF670 at 26.483 kb on - strand, within GFF670 at 26.483 kb on - strand, within GFF670 at 26.483 kb on - strand, within GFF670 at 26.483 kb on - strand, within GFF670 at 26.869 kb on - strand, within GFF670 at 26.971 kb on - strand, within GFF670 at 26.971 kb on - strand, within GFF670 at 26.973 kb on - strand, within GFF670 at 27.069 kb on - strand at 27.099 kb on - strand at 27.217 kb on + strand at 27.353 kb on - strand, within GFF671 at 27.362 kb on + strand, within GFF671 at 27.363 kb on - strand, within GFF671 at 27.404 kb on + strand, within GFF671 at 27.405 kb on - strand, within GFF671 at 27.467 kb on - strand, within GFF671 at 27.476 kb on - strand, within GFF671 at 27.681 kb on + strand, within GFF671 at 27.681 kb on + strand, within GFF671 at 27.723 kb on - strand, within GFF671 at 27.738 kb on - strand, within GFF671 at 27.741 kb on + strand, within GFF671 at 27.741 kb on + strand, within GFF671 at 27.742 kb on - strand, within GFF671 at 27.788 kb on + strand, within GFF671 at 27.789 kb on - strand, within GFF671 at 27.789 kb on - strand, within GFF671 at 28.005 kb on + strand, within GFF671 at 28.005 kb on + strand, within GFF671
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 25,146 + +1.6 25,147 - +0.4 25,233 + +0.1 25,233 + -0.2 25,234 - +0.6 25,234 - -0.3 25,446 + GFF669 0.26 -0.9 25,452 + GFF669 0.27 -0.2 25,455 - GFF669 0.27 -1.3 25,455 - GFF669 0.27 -0.8 25,714 - GFF669 0.82 +0.5 25,792 - -1.3 25,850 - -0.4 25,960 + -2.3 25,961 - -0.7 26,218 - GFF670 0.28 +0.4 26,235 + GFF670 0.29 +0.8 26,236 - GFF670 0.29 -0.8 26,408 - GFF670 0.43 -0.4 26,451 + GFF670 0.46 -1.4 26,452 - GFF670 0.46 +0.2 26,452 - GFF670 0.46 -0.2 26,458 - GFF670 0.47 +0.7 26,480 + GFF670 0.48 +1.2 26,480 + GFF670 0.48 +0.4 26,482 + GFF670 0.48 -0.2 26,482 + GFF670 0.48 -0.1 26,482 + GFF670 0.48 -0.0 26,483 - GFF670 0.48 +1.5 26,483 - GFF670 0.48 -0.3 26,483 - GFF670 0.48 +0.2 26,483 - GFF670 0.48 -0.2 26,869 - GFF670 0.78 -1.0 26,971 - GFF670 0.86 -0.9 26,971 - GFF670 0.86 -1.0 26,973 - GFF670 0.86 -1.1 27,069 - -2.1 27,099 - -0.2 27,217 + -0.4 27,353 - GFF671 0.11 +0.7 27,362 + GFF671 0.12 -0.9 27,363 - GFF671 0.12 -1.4 27,404 + GFF671 0.16 +1.1 27,405 - GFF671 0.16 +0.1 27,467 - GFF671 0.22 -0.8 27,476 - GFF671 0.23 +2.7 27,681 + GFF671 0.43 +0.5 27,681 + GFF671 0.43 +0.7 27,723 - GFF671 0.47 +0.6 27,738 - GFF671 0.48 +0.6 27,741 + GFF671 0.49 +0.7 27,741 + GFF671 0.49 -0.9 27,742 - GFF671 0.49 +0.5 27,788 + GFF671 0.53 +1.8 27,789 - GFF671 0.53 +4.0 27,789 - GFF671 0.53 -2.2 28,005 + GFF671 0.74 +0.4 28,005 + GFF671 0.74 -0.4
Or see this region's nucleotide sequence