Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF64 and GFF65 are separated by 100 nucleotides GFF65 and GFF66 are separated by 81 nucleotides GFF66 and GFF67 overlap by 1 nucleotides GFF67 and GFF68 are separated by 26 nucleotides
GFF64 - Putative inner membrane protein, at 47,857 to 48,480
GFF64
GFF65 - ErpA, essential respiratory protein A / probable iron binding protein from the HesB_IscA_SufA family, at 48,581 to 48,925
GFF65
GFF66 - H(+)/Cl(-) exchange transporter ClcA, at 49,007 to 50,428
GFF66
GFF67 - hypothetical protein, at 50,428 to 50,571
GFF67
GFF68 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 50,598 to 51,878
GFF68
Position (kb) 49
50
51 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 48.072 kb on + strand, within GFF64 at 48.072 kb on + strand, within GFF64 at 48.178 kb on - strand, within GFF64 at 48.206 kb on + strand, within GFF64 at 48.305 kb on - strand, within GFF64 at 48.319 kb on - strand, within GFF64 at 48.319 kb on - strand, within GFF64 at 48.354 kb on + strand, within GFF64 at 48.354 kb on + strand, within GFF64 at 48.481 kb on + strand at 48.481 kb on + strand at 48.534 kb on - strand at 48.985 kb on - strand at 49.095 kb on + strand at 49.096 kb on - strand at 49.096 kb on - strand at 49.096 kb on - strand at 49.174 kb on - strand, within GFF66 at 49.417 kb on + strand, within GFF66 at 49.418 kb on - strand, within GFF66 at 49.471 kb on + strand, within GFF66 at 49.471 kb on + strand, within GFF66 at 49.472 kb on - strand, within GFF66 at 49.472 kb on - strand, within GFF66 at 49.550 kb on - strand, within GFF66 at 49.595 kb on - strand, within GFF66 at 49.666 kb on - strand, within GFF66 at 49.736 kb on + strand, within GFF66 at 49.829 kb on - strand, within GFF66 at 49.997 kb on + strand, within GFF66 at 50.166 kb on - strand, within GFF66 at 50.294 kb on - strand at 50.448 kb on - strand, within GFF67 at 50.451 kb on - strand, within GFF67 at 50.553 kb on + strand, within GFF67
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 48,072 + GFF64 0.34 +0.5 48,072 + GFF64 0.34 +0.4 48,178 - GFF64 0.51 -0.4 48,206 + GFF64 0.56 -1.5 48,305 - GFF64 0.72 +2.2 48,319 - GFF64 0.74 -0.1 48,319 - GFF64 0.74 +0.2 48,354 + GFF64 0.80 +0.6 48,354 + GFF64 0.80 -1.0 48,481 + -0.2 48,481 + -0.6 48,534 - +2.1 48,985 - +1.5 49,095 + -0.8 49,096 - -0.4 49,096 - -0.5 49,096 - -1.6 49,174 - GFF66 0.12 -0.2 49,417 + GFF66 0.29 +0.1 49,418 - GFF66 0.29 -0.3 49,471 + GFF66 0.33 +0.0 49,471 + GFF66 0.33 +0.1 49,472 - GFF66 0.33 +0.8 49,472 - GFF66 0.33 +0.2 49,550 - GFF66 0.38 +1.7 49,595 - GFF66 0.41 -0.7 49,666 - GFF66 0.46 +3.2 49,736 + GFF66 0.51 +0.2 49,829 - GFF66 0.58 -0.0 49,997 + GFF66 0.70 +0.9 50,166 - GFF66 0.82 -3.6 50,294 - +0.1 50,448 - GFF67 0.14 +0.9 50,451 - GFF67 0.16 -0.4 50,553 + GFF67 0.87 +0.1
Or see this region's nucleotide sequence