Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF650 and GFF651 are separated by 2 nucleotides GFF651 and GFF652 are separated by 10 nucleotides
GFF650 - Galactokinase (EC 2.7.1.6), at 5,437 to 6,585
GFF650
GFF651 - Galactose-1-phosphate uridylyltransferase (EC 2.7.7.10), at 6,588 to 7,634
GFF651
GFF652 - UDP-N-acetylglucosamine 4-epimerase (EC 5.1.3.7) / UDP-glucose 4-epimerase (EC 5.1.3.2), at 7,645 to 8,661
GFF652
Position (kb) 6
7
8 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9
10 at 5.650 kb on + strand, within GFF650 at 5.719 kb on - strand, within GFF650 at 5.783 kb on - strand, within GFF650 at 5.783 kb on - strand, within GFF650 at 5.783 kb on - strand, within GFF650 at 5.913 kb on + strand, within GFF650 at 5.916 kb on + strand, within GFF650 at 5.916 kb on + strand, within GFF650 at 5.917 kb on - strand, within GFF650 at 5.919 kb on - strand, within GFF650 at 6.395 kb on - strand, within GFF650 at 6.423 kb on + strand, within GFF650 at 6.689 kb on - strand at 6.903 kb on - strand, within GFF651 at 7.033 kb on + strand, within GFF651 at 7.417 kb on + strand, within GFF651 at 7.418 kb on - strand, within GFF651 at 7.592 kb on + strand at 7.594 kb on + strand at 7.623 kb on - strand at 7.634 kb on + strand at 7.805 kb on + strand, within GFF652 at 7.831 kb on - strand, within GFF652 at 7.884 kb on + strand, within GFF652 at 7.886 kb on + strand, within GFF652 at 7.887 kb on - strand, within GFF652 at 7.890 kb on + strand, within GFF652 at 7.890 kb on + strand, within GFF652 at 8.053 kb on - strand, within GFF652 at 8.054 kb on + strand, within GFF652 at 8.054 kb on + strand, within GFF652 at 8.055 kb on - strand, within GFF652 at 8.206 kb on - strand, within GFF652 at 8.298 kb on + strand, within GFF652 at 8.313 kb on + strand, within GFF652
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 5,650 + GFF650 0.19 +1.2 5,719 - GFF650 0.25 -2.2 5,783 - GFF650 0.30 -1.3 5,783 - GFF650 0.30 -0.7 5,783 - GFF650 0.30 -1.3 5,913 + GFF650 0.41 -2.1 5,916 + GFF650 0.42 -0.2 5,916 + GFF650 0.42 +0.4 5,917 - GFF650 0.42 -0.1 5,919 - GFF650 0.42 +1.8 6,395 - GFF650 0.83 -0.8 6,423 + GFF650 0.86 +0.0 6,689 - -2.6 6,903 - GFF651 0.30 -0.2 7,033 + GFF651 0.43 -2.1 7,417 + GFF651 0.79 -1.6 7,418 - GFF651 0.79 -0.4 7,592 + +1.2 7,594 + +0.2 7,623 - -2.1 7,634 + +0.4 7,805 + GFF652 0.16 +8.5 7,831 - GFF652 0.18 -1.0 7,884 + GFF652 0.24 +9.3 7,886 + GFF652 0.24 +9.2 7,887 - GFF652 0.24 -1.6 7,890 + GFF652 0.24 +9.5 7,890 + GFF652 0.24 +10.5 8,053 - GFF652 0.40 -1.1 8,054 + GFF652 0.40 +9.4 8,054 + GFF652 0.40 +9.6 8,055 - GFF652 0.40 -1.0 8,206 - GFF652 0.55 +2.2 8,298 + GFF652 0.64 +9.4 8,313 + GFF652 0.66 +8.7
Or see this region's nucleotide sequence