Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF575 and GFF576 overlap by 4 nucleotides GFF576 and GFF577 are separated by 14 nucleotides
GFF575 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 304,797 to 306,983
GFF575
GFF576 - Glycogen debranching enzyme (EC 3.2.1.-), at 306,980 to 308,956
GFF576
GFF577 - Glucose-1-phosphate adenylyltransferase (EC 2.7.7.27), at 308,971 to 310,266
GFF577
Position (kb)
306
307
308
309 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 306.023 kb on + strand, within GFF575 at 306.030 kb on + strand, within GFF575 at 306.119 kb on + strand, within GFF575 at 306.231 kb on + strand, within GFF575 at 306.333 kb on + strand, within GFF575 at 306.454 kb on - strand, within GFF575 at 306.454 kb on - strand, within GFF575 at 306.455 kb on + strand, within GFF575 at 306.456 kb on + strand, within GFF575 at 306.457 kb on - strand, within GFF575 at 306.624 kb on + strand, within GFF575 at 306.635 kb on + strand, within GFF575 at 306.636 kb on - strand, within GFF575 at 306.636 kb on - strand, within GFF575 at 306.767 kb on + strand at 306.929 kb on + strand at 307.025 kb on + strand at 307.026 kb on - strand at 307.026 kb on - strand at 307.026 kb on - strand at 307.077 kb on + strand at 307.077 kb on + strand at 307.290 kb on + strand, within GFF576 at 307.385 kb on + strand, within GFF576 at 307.386 kb on - strand, within GFF576 at 307.440 kb on - strand, within GFF576 at 307.466 kb on + strand, within GFF576 at 307.494 kb on + strand, within GFF576 at 307.505 kb on + strand, within GFF576 at 307.505 kb on + strand, within GFF576 at 307.642 kb on + strand, within GFF576 at 307.642 kb on + strand, within GFF576 at 307.643 kb on - strand, within GFF576 at 307.768 kb on - strand, within GFF576 at 307.770 kb on + strand, within GFF576 at 307.834 kb on + strand, within GFF576 at 307.838 kb on + strand, within GFF576 at 307.838 kb on + strand, within GFF576 at 307.838 kb on + strand, within GFF576 at 307.839 kb on - strand, within GFF576 at 307.839 kb on - strand, within GFF576 at 307.839 kb on - strand, within GFF576 at 307.841 kb on + strand, within GFF576 at 308.396 kb on + strand, within GFF576 at 308.396 kb on + strand, within GFF576 at 308.443 kb on - strand, within GFF576 at 308.443 kb on - strand, within GFF576 at 308.557 kb on + strand, within GFF576 at 308.671 kb on - strand, within GFF576 at 308.680 kb on + strand, within GFF576 at 308.681 kb on - strand, within GFF576 at 308.767 kb on + strand at 308.767 kb on + strand at 308.768 kb on - strand at 308.836 kb on + strand at 308.836 kb on + strand at 308.836 kb on + strand at 308.836 kb on + strand at 308.839 kb on - strand at 308.982 kb on - strand at 309.000 kb on - strand at 309.038 kb on + strand at 309.039 kb on - strand at 309.144 kb on - strand, within GFF577 at 309.193 kb on + strand, within GFF577 at 309.193 kb on + strand, within GFF577 at 309.220 kb on - strand, within GFF577 at 309.291 kb on + strand, within GFF577 at 309.351 kb on + strand, within GFF577 at 309.351 kb on + strand, within GFF577 at 309.352 kb on - strand, within GFF577 at 309.374 kb on - strand, within GFF577 at 309.400 kb on - strand, within GFF577 at 309.400 kb on - strand, within GFF577 at 309.403 kb on + strand, within GFF577 at 309.404 kb on - strand, within GFF577 at 309.424 kb on - strand, within GFF577 at 309.424 kb on - strand, within GFF577 at 309.472 kb on - strand, within GFF577 at 309.640 kb on + strand, within GFF577 at 309.641 kb on - strand, within GFF577 at 309.800 kb on + strand, within GFF577 at 309.800 kb on + strand, within GFF577 at 309.801 kb on - strand, within GFF577 at 309.801 kb on - strand, within GFF577 at 309.804 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.891 kb on + strand, within GFF577 at 309.892 kb on - strand, within GFF577 at 309.898 kb on - strand, within GFF577 at 309.951 kb on + strand, within GFF577
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 306,023 + GFF575 0.56 -0.6 306,030 + GFF575 0.56 -2.6 306,119 + GFF575 0.60 -0.2 306,231 + GFF575 0.66 -0.4 306,333 + GFF575 0.70 +0.4 306,454 - GFF575 0.76 -0.1 306,454 - GFF575 0.76 +1.0 306,455 + GFF575 0.76 -2.3 306,456 + GFF575 0.76 +0.6 306,457 - GFF575 0.76 +0.8 306,624 + GFF575 0.84 -2.2 306,635 + GFF575 0.84 -1.2 306,636 - GFF575 0.84 -1.3 306,636 - GFF575 0.84 -0.2 306,767 + +0.3 306,929 + -1.2 307,025 + +0.2 307,026 - -0.4 307,026 - +0.8 307,026 - -1.0 307,077 + +2.9 307,077 + -1.0 307,290 + GFF576 0.16 -2.2 307,385 + GFF576 0.20 -0.7 307,386 - GFF576 0.21 -1.2 307,440 - GFF576 0.23 +0.4 307,466 + GFF576 0.25 +0.8 307,494 + GFF576 0.26 +0.6 307,505 + GFF576 0.27 -1.2 307,505 + GFF576 0.27 -1.3 307,642 + GFF576 0.33 +1.3 307,642 + GFF576 0.33 +0.6 307,643 - GFF576 0.34 -2.4 307,768 - GFF576 0.40 -0.2 307,770 + GFF576 0.40 -3.5 307,834 + GFF576 0.43 -2.4 307,838 + GFF576 0.43 +0.4 307,838 + GFF576 0.43 -1.8 307,838 + GFF576 0.43 +1.2 307,839 - GFF576 0.43 -0.0 307,839 - GFF576 0.43 +2.7 307,839 - GFF576 0.43 +0.6 307,841 + GFF576 0.44 +0.9 308,396 + GFF576 0.72 +0.3 308,396 + GFF576 0.72 -0.2 308,443 - GFF576 0.74 +0.6 308,443 - GFF576 0.74 -0.3 308,557 + GFF576 0.80 +1.3 308,671 - GFF576 0.86 -0.1 308,680 + GFF576 0.86 +2.2 308,681 - GFF576 0.86 +0.5 308,767 + -2.9 308,767 + +1.3 308,768 - -1.7 308,836 + +0.1 308,836 + +1.8 308,836 + +1.9 308,836 + -0.2 308,839 - +1.1 308,982 - +0.5 309,000 - +1.6 309,038 + +0.3 309,039 - -1.1 309,144 - GFF577 0.13 -0.6 309,193 + GFF577 0.17 -0.7 309,193 + GFF577 0.17 -0.3 309,220 - GFF577 0.19 +0.5 309,291 + GFF577 0.25 +0.1 309,351 + GFF577 0.29 -0.8 309,351 + GFF577 0.29 -0.0 309,352 - GFF577 0.29 +2.7 309,374 - GFF577 0.31 -0.7 309,400 - GFF577 0.33 +2.0 309,400 - GFF577 0.33 -0.2 309,403 + GFF577 0.33 +0.7 309,404 - GFF577 0.33 -0.6 309,424 - GFF577 0.35 +1.6 309,424 - GFF577 0.35 -0.2 309,472 - GFF577 0.39 -0.6 309,640 + GFF577 0.52 +2.0 309,641 - GFF577 0.52 +0.5 309,800 + GFF577 0.64 -0.5 309,800 + GFF577 0.64 +0.8 309,801 - GFF577 0.64 +0.1 309,801 - GFF577 0.64 +1.3 309,804 + GFF577 0.64 +0.8 309,868 + GFF577 0.69 -1.8 309,868 + GFF577 0.69 +1.7 309,868 + GFF577 0.69 -0.0 309,868 + GFF577 0.69 +1.3 309,869 - GFF577 0.69 -1.7 309,869 - GFF577 0.69 +0.2 309,869 - GFF577 0.69 +0.3 309,869 - GFF577 0.69 -1.4 309,891 + GFF577 0.71 -0.7 309,892 - GFF577 0.71 +0.6 309,898 - GFF577 0.72 +1.1 309,951 + GFF577 0.76 +0.7
Or see this region's nucleotide sequence