Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF548 and GFF549 are separated by 59 nucleotides GFF549 and GFF550 overlap by 4 nucleotides GFF550 and GFF551 overlap by 4 nucleotides GFF551 and GFF552 are separated by 1 nucleotides
GFF548 - High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1), at 279,639 to 280,685
GFF548
GFF549 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 280,745 to 281,671
GFF549
GFF550 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 281,668 to 282,945
GFF550
GFF551 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 282,942 to 283,709
GFF551
GFF552 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 283,711 to 284,424
GFF552
Position (kb) 281
282
283 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 280.697 kb on - strand at 280.697 kb on - strand at 280.740 kb on - strand at 280.744 kb on + strand at 280.745 kb on - strand at 280.822 kb on + strand at 280.831 kb on + strand at 280.831 kb on + strand at 280.831 kb on + strand at 280.832 kb on - strand at 280.842 kb on - strand, within GFF549 at 280.842 kb on - strand, within GFF549 at 280.883 kb on + strand, within GFF549 at 280.884 kb on - strand, within GFF549 at 280.884 kb on - strand, within GFF549 at 280.884 kb on - strand, within GFF549 at 280.884 kb on - strand, within GFF549 at 280.884 kb on - strand, within GFF549 at 280.885 kb on + strand, within GFF549 at 280.885 kb on + strand, within GFF549 at 280.885 kb on + strand, within GFF549 at 280.885 kb on + strand, within GFF549 at 280.886 kb on - strand, within GFF549 at 280.898 kb on + strand, within GFF549 at 280.899 kb on - strand, within GFF549 at 280.910 kb on - strand, within GFF549 at 280.942 kb on - strand, within GFF549 at 280.988 kb on - strand, within GFF549 at 280.988 kb on - strand, within GFF549 at 281.229 kb on + strand, within GFF549 at 281.341 kb on + strand, within GFF549 at 281.342 kb on - strand, within GFF549 at 281.452 kb on + strand, within GFF549 at 281.565 kb on - strand, within GFF549 at 281.568 kb on + strand, within GFF549 at 281.573 kb on - strand, within GFF549 at 281.581 kb on + strand at 281.582 kb on - strand at 281.582 kb on - strand at 281.582 kb on - strand at 281.744 kb on + strand at 281.745 kb on - strand at 282.029 kb on - strand, within GFF550 at 282.118 kb on + strand, within GFF550 at 282.118 kb on + strand, within GFF550 at 282.120 kb on + strand, within GFF550 at 282.146 kb on - strand, within GFF550 at 282.146 kb on - strand, within GFF550 at 282.286 kb on - strand, within GFF550 at 282.579 kb on - strand, within GFF550 at 282.616 kb on - strand, within GFF550 at 282.955 kb on + strand at 283.085 kb on + strand, within GFF551 at 283.104 kb on + strand, within GFF551 at 283.541 kb on + strand, within GFF551 at 283.580 kb on + strand, within GFF551 at 283.693 kb on - strand at 283.708 kb on - strand at 283.708 kb on - strand at 283.908 kb on + strand, within GFF552 at 283.917 kb on - strand, within GFF552
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 280,697 - -0.0 280,697 - +1.6 280,740 - +0.4 280,744 + +0.9 280,745 - +2.9 280,822 + +1.3 280,831 + +0.5 280,831 + -0.3 280,831 + +0.5 280,832 - +0.4 280,842 - GFF549 0.10 -0.0 280,842 - GFF549 0.10 -1.6 280,883 + GFF549 0.15 +0.6 280,884 - GFF549 0.15 +1.2 280,884 - GFF549 0.15 -1.9 280,884 - GFF549 0.15 +0.1 280,884 - GFF549 0.15 -0.2 280,884 - GFF549 0.15 -1.2 280,885 + GFF549 0.15 -0.0 280,885 + GFF549 0.15 +1.2 280,885 + GFF549 0.15 +0.0 280,885 + GFF549 0.15 -1.4 280,886 - GFF549 0.15 -1.0 280,898 + GFF549 0.17 +1.7 280,899 - GFF549 0.17 -0.9 280,910 - GFF549 0.18 +1.2 280,942 - GFF549 0.21 +0.4 280,988 - GFF549 0.26 -0.1 280,988 - GFF549 0.26 -0.5 281,229 + GFF549 0.52 +0.1 281,341 + GFF549 0.64 -2.8 281,342 - GFF549 0.64 +0.1 281,452 + GFF549 0.76 -0.2 281,565 - GFF549 0.88 +0.3 281,568 + GFF549 0.89 -0.0 281,573 - GFF549 0.89 -1.7 281,581 + -0.2 281,582 - +0.7 281,582 - -0.4 281,582 - +2.6 281,744 + +0.4 281,745 - +0.7 282,029 - GFF550 0.28 +0.6 282,118 + GFF550 0.35 -0.6 282,118 + GFF550 0.35 -0.8 282,120 + GFF550 0.35 +0.8 282,146 - GFF550 0.37 +1.2 282,146 - GFF550 0.37 +1.3 282,286 - GFF550 0.48 +1.4 282,579 - GFF550 0.71 +1.2 282,616 - GFF550 0.74 -0.5 282,955 + +0.3 283,085 + GFF551 0.19 -2.8 283,104 + GFF551 0.21 -0.3 283,541 + GFF551 0.78 -0.6 283,580 + GFF551 0.83 +0.7 283,693 - +2.2 283,708 - +1.1 283,708 - -0.5 283,908 + GFF552 0.28 -0.9 283,917 - GFF552 0.29 -1.7
Or see this region's nucleotide sequence