Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF520 and GFF521 are separated by 441 nucleotides GFF521 and GFF522 are separated by 139 nucleotides
GFF520 - Universal stress protein A, at 251,789 to 252,223
GFF520
GFF521 - Universal stress protein B, at 252,665 to 252,946
GFF521
GFF522 - Low-affinity inorganic phosphate transporter, at 253,086 to 254,582
GFF522
Position (kb) 252
253 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 251.745 kb on + strand at 251.745 kb on + strand at 251.747 kb on + strand at 251.748 kb on - strand at 251.788 kb on - strand at 251.788 kb on - strand at 251.788 kb on - strand at 251.920 kb on - strand, within GFF520 at 251.920 kb on - strand, within GFF520 at 251.983 kb on + strand, within GFF520 at 252.052 kb on + strand, within GFF520 at 252.217 kb on - strand at 252.240 kb on + strand at 252.241 kb on - strand at 252.241 kb on - strand at 252.322 kb on - strand at 252.343 kb on + strand at 252.343 kb on + strand at 252.343 kb on + strand at 252.344 kb on - strand at 252.349 kb on + strand at 252.349 kb on + strand at 252.350 kb on - strand at 252.402 kb on - strand at 252.508 kb on + strand at 252.523 kb on + strand at 252.523 kb on + strand at 252.526 kb on + strand at 252.527 kb on - strand at 252.610 kb on + strand at 252.611 kb on - strand at 252.615 kb on + strand at 252.630 kb on + strand at 252.642 kb on + strand at 252.642 kb on + strand at 252.642 kb on + strand at 252.664 kb on + strand at 252.674 kb on + strand at 252.674 kb on + strand at 252.731 kb on + strand, within GFF521 at 252.732 kb on - strand, within GFF521 at 252.737 kb on + strand, within GFF521 at 252.737 kb on + strand, within GFF521 at 252.798 kb on + strand, within GFF521 at 252.799 kb on - strand, within GFF521 at 252.803 kb on + strand, within GFF521 at 252.803 kb on + strand, within GFF521 at 252.803 kb on + strand, within GFF521 at 252.803 kb on + strand, within GFF521 at 252.803 kb on + strand, within GFF521 at 252.804 kb on - strand, within GFF521 at 252.804 kb on - strand, within GFF521 at 252.804 kb on - strand, within GFF521 at 252.805 kb on + strand, within GFF521 at 252.805 kb on + strand, within GFF521 at 252.809 kb on + strand, within GFF521 at 252.810 kb on - strand, within GFF521 at 252.982 kb on - strand at 252.985 kb on - strand at 252.988 kb on + strand at 253.011 kb on - strand at 253.148 kb on + strand at 253.148 kb on + strand at 253.239 kb on + strand, within GFF522 at 253.240 kb on - strand, within GFF522 at 253.240 kb on - strand, within GFF522 at 253.240 kb on - strand, within GFF522 at 253.286 kb on + strand, within GFF522 at 253.361 kb on + strand, within GFF522 at 253.425 kb on - strand, within GFF522 at 253.444 kb on + strand, within GFF522 at 253.463 kb on + strand, within GFF522 at 253.574 kb on + strand, within GFF522 at 253.591 kb on - strand, within GFF522 at 253.591 kb on - strand, within GFF522 at 253.591 kb on - strand, within GFF522
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 251,745 + +0.4 251,745 + -2.8 251,747 + +0.3 251,748 - +0.5 251,788 - -1.5 251,788 - +0.3 251,788 - +0.4 251,920 - GFF520 0.30 +0.6 251,920 - GFF520 0.30 +2.1 251,983 + GFF520 0.45 -3.1 252,052 + GFF520 0.60 -0.7 252,217 - +2.0 252,240 + +0.6 252,241 - -0.2 252,241 - -0.3 252,322 - +1.1 252,343 + +0.2 252,343 + -3.8 252,343 + -0.2 252,344 - +0.1 252,349 + +0.3 252,349 + +1.4 252,350 - +1.0 252,402 - -0.5 252,508 + +0.8 252,523 + -1.5 252,523 + +0.1 252,526 + -1.8 252,527 - +0.5 252,610 + -0.5 252,611 - -0.6 252,615 + -1.3 252,630 + +0.4 252,642 + +0.1 252,642 + +0.6 252,642 + +0.8 252,664 + -0.3 252,674 + +1.4 252,674 + -1.1 252,731 + GFF521 0.23 +0.3 252,732 - GFF521 0.24 +2.2 252,737 + GFF521 0.26 +1.0 252,737 + GFF521 0.26 -0.1 252,798 + GFF521 0.47 -0.7 252,799 - GFF521 0.48 -0.5 252,803 + GFF521 0.49 +0.7 252,803 + GFF521 0.49 +2.0 252,803 + GFF521 0.49 -0.3 252,803 + GFF521 0.49 -2.0 252,803 + GFF521 0.49 +0.9 252,804 - GFF521 0.49 +0.6 252,804 - GFF521 0.49 -0.7 252,804 - GFF521 0.49 +1.3 252,805 + GFF521 0.50 +0.4 252,805 + GFF521 0.50 +0.3 252,809 + GFF521 0.51 +0.8 252,810 - GFF521 0.51 +1.1 252,982 - -0.9 252,985 - -3.8 252,988 + +0.1 253,011 - -2.2 253,148 + -1.7 253,148 + -2.4 253,239 + GFF522 0.10 +1.5 253,240 - GFF522 0.10 -3.1 253,240 - GFF522 0.10 +1.6 253,240 - GFF522 0.10 -1.5 253,286 + GFF522 0.13 -2.1 253,361 + GFF522 0.18 -2.6 253,425 - GFF522 0.23 +0.6 253,444 + GFF522 0.24 -0.5 253,463 + GFF522 0.25 -0.4 253,574 + GFF522 0.33 -0.4 253,591 - GFF522 0.34 +1.9 253,591 - GFF522 0.34 +1.8 253,591 - GFF522 0.34 +2.2
Or see this region's nucleotide sequence