Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4867 and GFF4868 overlap by 1 nucleotides GFF4868 and GFF4869 are separated by 272 nucleotides GFF4869 and GFF4870 are separated by 51 nucleotides GFF4870 and GFF4871 are separated by 109 nucleotides
GFF4867 - Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA, at 165,921 to 167,126
GFF4867
GFF4868 - Cysteine desulfurase CsdA-CsdE, sulfur acceptor protein CsdE, at 167,126 to 167,569
GFF4868
GFF4869 - FIG00761799: membrane protein, at 167,842 to 168,756
GFF4869
GFF4870 - CsdL (EC-YgdL) protein of the HesA/MoeB/ThiF family, part of the CsdA-E-L sulfur transfer pathway, at 168,808 to 169,614
GFF4870
GFF4871 - Membrane-bound lytic murein transglycosylase A precursor (EC 3.2.1.-), at 169,724 to 170,821
GFF4871
Position (kb) 167
168
169 Strain fitness (log2 ratio) -1
0
1
2 at 166.963 kb on - strand, within GFF4867 at 167.057 kb on + strand at 167.057 kb on + strand at 167.124 kb on + strand at 167.141 kb on + strand at 167.488 kb on - strand, within GFF4868 at 167.582 kb on - strand at 168.302 kb on + strand, within GFF4869 at 168.351 kb on - strand, within GFF4869 at 168.351 kb on - strand, within GFF4869 at 168.510 kb on - strand, within GFF4869 at 168.672 kb on - strand at 169.024 kb on - strand, within GFF4870 at 169.053 kb on + strand, within GFF4870 at 169.169 kb on - strand, within GFF4870 at 169.180 kb on - strand, within GFF4870 at 169.181 kb on + strand, within GFF4870 at 169.244 kb on + strand, within GFF4870 at 169.244 kb on + strand, within GFF4870 at 169.245 kb on - strand, within GFF4870 at 169.249 kb on - strand, within GFF4870 at 169.601 kb on - strand at 169.601 kb on - strand at 169.614 kb on + strand at 169.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 166,963 - GFF4867 0.86 +1.5 167,057 + -0.8 167,057 + -0.1 167,124 + -0.9 167,141 + -0.6 167,488 - GFF4868 0.82 +1.7 167,582 - -0.1 168,302 + GFF4869 0.50 -0.2 168,351 - GFF4869 0.56 -0.1 168,351 - GFF4869 0.56 +2.0 168,510 - GFF4869 0.73 -0.8 168,672 - -0.4 169,024 - GFF4870 0.27 -0.5 169,053 + GFF4870 0.30 +0.2 169,169 - GFF4870 0.45 -0.1 169,180 - GFF4870 0.46 +0.1 169,181 + GFF4870 0.46 -0.7 169,244 + GFF4870 0.54 -0.4 169,244 + GFF4870 0.54 -0.8 169,245 - GFF4870 0.54 +0.3 169,249 - GFF4870 0.55 -0.4 169,601 - -0.7 169,601 - -1.7 169,614 + +0.5 169,615 - -0.5
Or see this region's nucleotide sequence