Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4699 and GFF4700 are separated by 75 nucleotides GFF4700 and GFF4701 are separated by 197 nucleotides GFF4701 and GFF4702 are separated by 31 nucleotides GFF4702 and GFF4703 overlap by 4 nucleotides
GFF4699 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140), at 10,005 to 10,784
GFF4699
GFF4700 - Glucitol operon activator protein, at 10,860 to 11,219
GFF4700
GFF4701 - Glucitol operon repressor, at 11,417 to 12,190
GFF4701
GFF4702 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 12,222 to 13,148
GFF4702
GFF4703 - Anaerobic nitric oxide reductase transcription regulator NorR, at 13,145 to 14,665
GFF4703
Position (kb)
11
12
13 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 10.428 kb on - strand, within GFF4699 at 10.540 kb on + strand, within GFF4699 at 10.645 kb on - strand, within GFF4699 at 10.647 kb on + strand, within GFF4699 at 10.647 kb on + strand, within GFF4699 at 10.648 kb on - strand, within GFF4699 at 10.648 kb on - strand, within GFF4699 at 10.714 kb on - strand at 10.859 kb on + strand at 10.860 kb on - strand at 10.961 kb on + strand, within GFF4700 at 11.006 kb on + strand, within GFF4700 at 11.323 kb on + strand at 11.323 kb on + strand at 11.323 kb on + strand at 11.323 kb on + strand at 11.380 kb on - strand at 11.393 kb on - strand at 11.396 kb on + strand at 11.498 kb on + strand, within GFF4701 at 11.574 kb on - strand, within GFF4701 at 11.678 kb on + strand, within GFF4701 at 11.678 kb on + strand, within GFF4701 at 11.679 kb on - strand, within GFF4701 at 11.727 kb on + strand, within GFF4701 at 11.789 kb on - strand, within GFF4701 at 12.196 kb on + strand at 12.199 kb on + strand at 12.220 kb on + strand at 12.468 kb on - strand, within GFF4702 at 12.468 kb on - strand, within GFF4702 at 13.005 kb on - strand, within GFF4702 at 13.064 kb on + strand at 13.142 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 10,428 - GFF4699 0.54 +0.9 10,540 + GFF4699 0.69 +0.1 10,645 - GFF4699 0.82 -3.0 10,647 + GFF4699 0.82 +0.9 10,647 + GFF4699 0.82 +1.6 10,648 - GFF4699 0.82 +0.9 10,648 - GFF4699 0.82 +0.7 10,714 - +0.1 10,859 + +1.2 10,860 - -1.7 10,961 + GFF4700 0.28 -0.3 11,006 + GFF4700 0.41 -0.2 11,323 + +0.6 11,323 + +0.8 11,323 + -0.3 11,323 + +0.6 11,380 - -1.3 11,393 - +0.1 11,396 + -0.8 11,498 + GFF4701 0.10 +0.2 11,574 - GFF4701 0.20 -1.1 11,678 + GFF4701 0.34 +0.2 11,678 + GFF4701 0.34 -0.0 11,679 - GFF4701 0.34 +0.1 11,727 + GFF4701 0.40 +0.1 11,789 - GFF4701 0.48 +0.9 12,196 + +0.7 12,199 + -0.6 12,220 + -1.0 12,468 - GFF4702 0.27 +0.8 12,468 - GFF4702 0.27 -0.5 13,005 - GFF4702 0.84 -0.7 13,064 + +0.2 13,142 + +1.5
Or see this region's nucleotide sequence