Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4630 and GFF4631 are separated by 67 nucleotides GFF4631 and GFF4632 are separated by 48 nucleotides GFF4632 and GFF4633 are separated by 54 nucleotides
GFF4630 - Dolichol-phosphate mannosyltransferase (EC 2.4.1.83) in lipid-linked oligosaccharide synthesis cluster, at 422,266 to 424,215
GFF4630
GFF4631 - hypothetical protein, at 424,283 to 424,420
GFF4631
GFF4632 - Endonuclease VIII, at 424,469 to 425,260
GFF4632
GFF4633 - Putative transport protein, at 425,315 to 426,361
GFF4633
Position (kb) 424
425
426 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 424.342 kb on + strand, within GFF4631 at 424.342 kb on + strand, within GFF4631 at 424.345 kb on + strand, within GFF4631 at 424.466 kb on - strand at 424.566 kb on + strand, within GFF4632 at 424.633 kb on - strand, within GFF4632 at 424.683 kb on - strand, within GFF4632 at 424.732 kb on + strand, within GFF4632 at 424.732 kb on + strand, within GFF4632 at 424.733 kb on - strand, within GFF4632 at 424.734 kb on + strand, within GFF4632 at 424.768 kb on + strand, within GFF4632 at 424.769 kb on - strand, within GFF4632 at 424.977 kb on - strand, within GFF4632 at 425.024 kb on - strand, within GFF4632 at 425.097 kb on - strand, within GFF4632 at 425.346 kb on - strand at 425.346 kb on - strand at 425.497 kb on + strand, within GFF4633 at 425.514 kb on + strand, within GFF4633 at 425.515 kb on - strand, within GFF4633 at 425.645 kb on + strand, within GFF4633 at 425.655 kb on + strand, within GFF4633 at 425.655 kb on + strand, within GFF4633 at 425.813 kb on + strand, within GFF4633 at 425.814 kb on - strand, within GFF4633 at 425.927 kb on + strand, within GFF4633 at 425.928 kb on - strand, within GFF4633 at 425.931 kb on + strand, within GFF4633 at 426.172 kb on + strand, within GFF4633 at 426.230 kb on - strand, within GFF4633
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 424,342 + GFF4631 0.43 +0.2 424,342 + GFF4631 0.43 +0.2 424,345 + GFF4631 0.45 +0.4 424,466 - -0.1 424,566 + GFF4632 0.12 -0.2 424,633 - GFF4632 0.21 +0.6 424,683 - GFF4632 0.27 +1.3 424,732 + GFF4632 0.33 -0.2 424,732 + GFF4632 0.33 +1.8 424,733 - GFF4632 0.33 +1.3 424,734 + GFF4632 0.33 -0.1 424,768 + GFF4632 0.38 +0.4 424,769 - GFF4632 0.38 +1.7 424,977 - GFF4632 0.64 -1.5 425,024 - GFF4632 0.70 +1.8 425,097 - GFF4632 0.79 -3.2 425,346 - -0.3 425,346 - +0.2 425,497 + GFF4633 0.17 +2.2 425,514 + GFF4633 0.19 +0.6 425,515 - GFF4633 0.19 -1.0 425,645 + GFF4633 0.32 +0.5 425,655 + GFF4633 0.32 +0.9 425,655 + GFF4633 0.32 -0.5 425,813 + GFF4633 0.48 +0.6 425,814 - GFF4633 0.48 +0.7 425,927 + GFF4633 0.58 +0.8 425,928 - GFF4633 0.59 +0.9 425,931 + GFF4633 0.59 +0.9 426,172 + GFF4633 0.82 +1.6 426,230 - GFF4633 0.87 -2.8
Or see this region's nucleotide sequence