Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4593 and GFF4594 overlap by 14 nucleotides GFF4594 and GFF4595 are separated by 97 nucleotides
GFF4593 - TcuB: works with TcuA to oxidize tricarballylate to cis-aconitate, at 383,270 to 384,409
GFF4593
GFF4594 - TcuA: flavoprotein used to oxidize tricarballylate to cis-aconitate, at 384,396 to 385,799
GFF4594
GFF4595 - TcuR: regulates tcuABC genes used in utilization of tricarballylate, at 385,897 to 386,823
GFF4595
Position (kb) 384
385
386 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 383.662 kb on - strand, within GFF4593 at 383.705 kb on - strand, within GFF4593 at 383.886 kb on - strand, within GFF4593 at 383.950 kb on + strand, within GFF4593 at 384.084 kb on + strand, within GFF4593 at 384.220 kb on + strand, within GFF4593 at 384.241 kb on - strand, within GFF4593 at 384.445 kb on - strand at 384.614 kb on + strand, within GFF4594 at 384.646 kb on + strand, within GFF4594 at 384.763 kb on + strand, within GFF4594 at 384.831 kb on - strand, within GFF4594 at 385.158 kb on - strand, within GFF4594 at 385.269 kb on + strand, within GFF4594 at 385.400 kb on - strand, within GFF4594 at 385.621 kb on - strand, within GFF4594 at 385.621 kb on - strand, within GFF4594 at 385.649 kb on + strand, within GFF4594 at 385.650 kb on - strand, within GFF4594 at 385.759 kb on - strand at 385.789 kb on - strand at 385.802 kb on + strand at 385.803 kb on - strand at 385.804 kb on + strand at 385.805 kb on - strand at 386.480 kb on + strand, within GFF4595 at 386.511 kb on + strand, within GFF4595 at 386.772 kb on + strand at 386.791 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 383,662 - GFF4593 0.34 -0.9 383,705 - GFF4593 0.38 +0.2 383,886 - GFF4593 0.54 +0.1 383,950 + GFF4593 0.60 +0.6 384,084 + GFF4593 0.71 +0.3 384,220 + GFF4593 0.83 -1.1 384,241 - GFF4593 0.85 -1.2 384,445 - -0.6 384,614 + GFF4594 0.16 -1.0 384,646 + GFF4594 0.18 +1.1 384,763 + GFF4594 0.26 +0.4 384,831 - GFF4594 0.31 +0.3 385,158 - GFF4594 0.54 +1.9 385,269 + GFF4594 0.62 +0.6 385,400 - GFF4594 0.72 -1.2 385,621 - GFF4594 0.87 +0.0 385,621 - GFF4594 0.87 +1.1 385,649 + GFF4594 0.89 +1.1 385,650 - GFF4594 0.89 +1.2 385,759 - +0.7 385,789 - -1.1 385,802 + -0.0 385,803 - -0.5 385,804 + -2.8 385,805 - +0.5 386,480 + GFF4595 0.63 -0.7 386,511 + GFF4595 0.66 -0.2 386,772 + -1.0 386,791 - -1.6
Or see this region's nucleotide sequence