Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4582 and GFF4583 are separated by 289 nucleotides GFF4583 and GFF4584 are separated by 46 nucleotides
GFF4582 - Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4), at 369,483 to 371,147
GFF4582
GFF4583 - Phosphatase NagD predicted to act in N-acetylglucosamine utilization subsystem, at 371,437 to 372,189
GFF4583
GFF4584 - N-acetylglucosamine-6P-responsive transcriptional repressor NagC, ROK family, at 372,236 to 373,456
GFF4584
Position (kb) 371
372
373 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 370.574 kb on - strand, within GFF4582 at 370.577 kb on - strand, within GFF4582 at 370.577 kb on - strand, within GFF4582 at 370.631 kb on + strand, within GFF4582 at 370.678 kb on + strand, within GFF4582 at 370.708 kb on + strand, within GFF4582 at 370.708 kb on + strand, within GFF4582 at 370.708 kb on + strand, within GFF4582 at 370.709 kb on - strand, within GFF4582 at 370.709 kb on - strand, within GFF4582 at 370.722 kb on - strand, within GFF4582 at 370.828 kb on + strand, within GFF4582 at 370.913 kb on - strand, within GFF4582 at 370.935 kb on + strand, within GFF4582 at 370.935 kb on + strand, within GFF4582 at 370.936 kb on - strand, within GFF4582 at 370.958 kb on - strand, within GFF4582 at 371.033 kb on - strand at 371.148 kb on - strand at 371.148 kb on - strand at 371.379 kb on + strand at 371.380 kb on - strand at 371.412 kb on - strand at 371.450 kb on - strand at 371.469 kb on - strand at 371.503 kb on - strand at 371.598 kb on + strand, within GFF4583 at 371.672 kb on + strand, within GFF4583 at 371.673 kb on - strand, within GFF4583 at 371.805 kb on + strand, within GFF4583 at 371.890 kb on - strand, within GFF4583 at 371.891 kb on + strand, within GFF4583 at 371.983 kb on - strand, within GFF4583 at 371.985 kb on - strand, within GFF4583 at 372.060 kb on - strand, within GFF4583 at 372.173 kb on - strand at 372.195 kb on - strand at 372.213 kb on + strand at 372.224 kb on + strand at 372.224 kb on + strand at 372.227 kb on - strand at 372.227 kb on - strand at 372.287 kb on - strand at 372.336 kb on - strand at 372.553 kb on + strand, within GFF4584 at 372.611 kb on - strand, within GFF4584 at 372.844 kb on - strand, within GFF4584 at 373.042 kb on + strand, within GFF4584 at 373.043 kb on - strand, within GFF4584 at 373.135 kb on + strand, within GFF4584 at 373.135 kb on + strand, within GFF4584 at 373.150 kb on - strand, within GFF4584 at 373.158 kb on - strand, within GFF4584
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 370,574 - GFF4582 0.66 +2.7 370,577 - GFF4582 0.66 -1.0 370,577 - GFF4582 0.66 +0.7 370,631 + GFF4582 0.69 +1.6 370,678 + GFF4582 0.72 +0.6 370,708 + GFF4582 0.74 -0.5 370,708 + GFF4582 0.74 -0.5 370,708 + GFF4582 0.74 -1.9 370,709 - GFF4582 0.74 -3.6 370,709 - GFF4582 0.74 -0.2 370,722 - GFF4582 0.74 +0.7 370,828 + GFF4582 0.81 +4.3 370,913 - GFF4582 0.86 -1.1 370,935 + GFF4582 0.87 -1.2 370,935 + GFF4582 0.87 +0.1 370,936 - GFF4582 0.87 -1.2 370,958 - GFF4582 0.89 +0.3 371,033 - +1.2 371,148 - +1.2 371,148 - +0.5 371,379 + +0.2 371,380 - +0.7 371,412 - +0.3 371,450 - -0.0 371,469 - -1.9 371,503 - +0.1 371,598 + GFF4583 0.21 -0.3 371,672 + GFF4583 0.31 -0.4 371,673 - GFF4583 0.31 +0.1 371,805 + GFF4583 0.49 -0.7 371,890 - GFF4583 0.60 -1.1 371,891 + GFF4583 0.60 -0.0 371,983 - GFF4583 0.73 -0.5 371,985 - GFF4583 0.73 -3.3 372,060 - GFF4583 0.83 +0.4 372,173 - +0.0 372,195 - +1.2 372,213 + -2.8 372,224 + -1.1 372,224 + -0.3 372,227 - -2.7 372,227 - -0.6 372,287 - -0.3 372,336 - -0.6 372,553 + GFF4584 0.26 +0.5 372,611 - GFF4584 0.31 -0.6 372,844 - GFF4584 0.50 -0.8 373,042 + GFF4584 0.66 +0.2 373,043 - GFF4584 0.66 -1.7 373,135 + GFF4584 0.74 +1.6 373,135 + GFF4584 0.74 +1.1 373,150 - GFF4584 0.75 -0.2 373,158 - GFF4584 0.76 -0.9
Or see this region's nucleotide sequence