Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4566 and GFF4567 are separated by 21 nucleotides GFF4567 and GFF4568 are separated by 75 nucleotides
GFF4566 - FIG00638911: hypothetical protein, at 351,253 to 352,725
GFF4566
GFF4567 - Chaperone protein DnaK, at 352,747 to 354,426
GFF4567
GFF4568 - putative cytoplasmic protein, at 354,502 to 355,740
GFF4568
Position (kb) 352
353
354
355 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 351.891 kb on + strand, within GFF4566 at 351.906 kb on + strand, within GFF4566 at 352.009 kb on + strand, within GFF4566 at 352.010 kb on - strand, within GFF4566 at 352.010 kb on - strand, within GFF4566 at 352.010 kb on - strand, within GFF4566 at 352.012 kb on + strand, within GFF4566 at 352.202 kb on - strand, within GFF4566 at 352.202 kb on - strand, within GFF4566 at 352.818 kb on + strand at 352.825 kb on - strand at 352.825 kb on - strand at 352.854 kb on + strand at 352.942 kb on - strand, within GFF4567 at 353.089 kb on - strand, within GFF4567 at 353.097 kb on + strand, within GFF4567 at 353.097 kb on + strand, within GFF4567 at 353.198 kb on + strand, within GFF4567 at 353.243 kb on + strand, within GFF4567 at 353.301 kb on + strand, within GFF4567 at 353.727 kb on - strand, within GFF4567 at 353.727 kb on - strand, within GFF4567 at 353.828 kb on + strand, within GFF4567 at 353.833 kb on - strand, within GFF4567 at 353.944 kb on - strand, within GFF4567 at 354.030 kb on + strand, within GFF4567 at 354.031 kb on - strand, within GFF4567 at 354.036 kb on + strand, within GFF4567 at 354.036 kb on + strand, within GFF4567 at 354.145 kb on + strand, within GFF4567 at 354.519 kb on - strand at 354.883 kb on - strand, within GFF4568 at 354.903 kb on + strand, within GFF4568 at 355.108 kb on - strand, within GFF4568 at 355.149 kb on + strand, within GFF4568 at 355.263 kb on - strand, within GFF4568 at 355.389 kb on - strand, within GFF4568 at 355.391 kb on + strand, within GFF4568
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 351,891 + GFF4566 0.43 +1.5 351,906 + GFF4566 0.44 -2.1 352,009 + GFF4566 0.51 -0.8 352,010 - GFF4566 0.51 +0.2 352,010 - GFF4566 0.51 -1.7 352,010 - GFF4566 0.51 +2.0 352,012 + GFF4566 0.52 +0.2 352,202 - GFF4566 0.64 -1.0 352,202 - GFF4566 0.64 +0.2 352,818 + -0.3 352,825 - -1.3 352,825 - +0.7 352,854 + -0.0 352,942 - GFF4567 0.12 +0.2 353,089 - GFF4567 0.20 -0.5 353,097 + GFF4567 0.21 +0.1 353,097 + GFF4567 0.21 -1.9 353,198 + GFF4567 0.27 +0.8 353,243 + GFF4567 0.30 -1.6 353,301 + GFF4567 0.33 -2.1 353,727 - GFF4567 0.58 +1.7 353,727 - GFF4567 0.58 -2.5 353,828 + GFF4567 0.64 -0.3 353,833 - GFF4567 0.65 +1.4 353,944 - GFF4567 0.71 -0.5 354,030 + GFF4567 0.76 -1.7 354,031 - GFF4567 0.76 +1.0 354,036 + GFF4567 0.77 -1.3 354,036 + GFF4567 0.77 -1.2 354,145 + GFF4567 0.83 -0.4 354,519 - +0.3 354,883 - GFF4568 0.31 -1.8 354,903 + GFF4568 0.32 -1.4 355,108 - GFF4568 0.49 -3.4 355,149 + GFF4568 0.52 +0.0 355,263 - GFF4568 0.61 +0.1 355,389 - GFF4568 0.72 +0.5 355,391 + GFF4568 0.72 -0.3
Or see this region's nucleotide sequence