Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF454 and GFF455 are separated by 288 nucleotides GFF455 and GFF456 are separated by 157 nucleotides GFF456 and GFF457 are separated by 149 nucleotides GFF457 and GFF458 are separated by 49 nucleotides
GFF454 - Cold shock protein CspA, at 176,427 to 176,639
GFF454
GFF455 - FIG00638451: hypothetical protein, at 176,928 to 177,218
GFF455
GFF456 - hypothetical protein, at 177,376 to 177,507
GFF456
GFF457 - probable exported protein YPO4070, at 177,657 to 178,367
GFF457
GFF458 - 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 178,417 to 179,391
GFF458
Position (kb)
177
178 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 176.559 kb on - strand, within GFF454 at 176.577 kb on - strand, within GFF454 at 176.580 kb on - strand, within GFF454 at 176.614 kb on - strand, within GFF454 at 176.640 kb on - strand at 176.640 kb on - strand at 176.645 kb on - strand at 176.648 kb on - strand at 176.768 kb on - strand at 176.848 kb on + strand at 176.894 kb on - strand at 176.949 kb on + strand at 176.950 kb on - strand at 177.097 kb on - strand, within GFF455 at 177.209 kb on + strand at 177.391 kb on - strand, within GFF456 at 177.434 kb on + strand, within GFF456 at 177.529 kb on + strand at 177.653 kb on - strand at 177.710 kb on - strand at 177.710 kb on - strand at 177.710 kb on - strand at 177.719 kb on + strand at 177.864 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.877 kb on - strand, within GFF457 at 177.877 kb on - strand, within GFF457 at 177.967 kb on - strand, within GFF457 at 178.206 kb on + strand, within GFF457 at 178.206 kb on + strand, within GFF457 at 178.284 kb on + strand, within GFF457 at 178.284 kb on + strand, within GFF457 at 178.329 kb on - strand at 178.352 kb on - strand at 178.483 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 176,559 - GFF454 0.62 +1.7 176,577 - GFF454 0.70 -1.1 176,580 - GFF454 0.72 -0.8 176,614 - GFF454 0.88 -0.5 176,640 - -0.1 176,640 - -0.1 176,645 - -2.7 176,648 - +0.3 176,768 - -1.0 176,848 + +0.1 176,894 - +0.6 176,949 + -1.1 176,950 - -0.6 177,097 - GFF455 0.58 +0.7 177,209 + -1.3 177,391 - GFF456 0.11 +0.2 177,434 + GFF456 0.44 +0.4 177,529 + +2.9 177,653 - +0.5 177,710 - +2.5 177,710 - +0.7 177,710 - +1.2 177,719 + -0.1 177,864 + GFF457 0.29 -3.0 177,876 + GFF457 0.31 +0.8 177,876 + GFF457 0.31 +0.8 177,876 + GFF457 0.31 +0.8 177,876 + GFF457 0.31 -0.1 177,877 - GFF457 0.31 -0.3 177,877 - GFF457 0.31 -0.4 177,967 - GFF457 0.44 +0.7 178,206 + GFF457 0.77 -0.3 178,206 + GFF457 0.77 -0.6 178,284 + GFF457 0.88 +0.4 178,284 + GFF457 0.88 -3.0 178,329 - +0.4 178,352 - +0.2 178,483 - -0.3
Or see this region's nucleotide sequence