Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4516 and GFF4517 overlap by 4 nucleotides GFF4517 and GFF4518 overlap by 1 nucleotides GFF4518 and GFF4519 are separated by 67 nucleotides GFF4519 and GFF4520 are separated by 21 nucleotides GFF4520 and GFF4521 are separated by 84 nucleotides
GFF4516 - Putative oxidoreductase STM0611-0613, chain A, at 304,149 to 306,197
GFF4516
GFF4517 - Anaerobic dimethyl sulfoxide reductase chain B (EC 1.8.5.3), at 306,194 to 306,751
GFF4517
GFF4518 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3), at 306,751 to 307,518
GFF4518
GFF4519 - Universal stress protein G, at 307,586 to 307,879
GFF4519
GFF4520 - FIG01045303: hypothetical protein, at 307,901 to 308,152
GFF4520
GFF4521 - Uncharacterized zinc-type alcohol dehydrogenase-like protein ybdR, at 308,237 to 309,475
GFF4521
Position (kb) 306
307
308 Strain fitness (log2 ratio) -2
-1
0
1
2 at 305.908 kb on - strand, within GFF4516 at 306.040 kb on - strand at 306.040 kb on - strand at 306.066 kb on + strand at 306.141 kb on - strand at 306.223 kb on + strand at 306.326 kb on + strand, within GFF4517 at 306.326 kb on + strand, within GFF4517 at 306.327 kb on - strand, within GFF4517 at 306.327 kb on - strand, within GFF4517 at 306.445 kb on + strand, within GFF4517 at 306.485 kb on - strand, within GFF4517 at 306.511 kb on + strand, within GFF4517 at 306.512 kb on - strand, within GFF4517 at 306.544 kb on + strand, within GFF4517 at 306.544 kb on + strand, within GFF4517 at 306.682 kb on + strand, within GFF4517 at 306.712 kb on + strand at 306.791 kb on + strand at 306.868 kb on + strand, within GFF4518 at 306.868 kb on + strand, within GFF4518 at 306.906 kb on + strand, within GFF4518 at 306.946 kb on + strand, within GFF4518 at 306.947 kb on - strand, within GFF4518 at 306.947 kb on - strand, within GFF4518 at 306.980 kb on + strand, within GFF4518 at 307.261 kb on - strand, within GFF4518 at 307.352 kb on - strand, within GFF4518 at 307.491 kb on + strand at 307.574 kb on + strand at 307.588 kb on - strand at 307.673 kb on - strand, within GFF4519 at 307.763 kb on - strand, within GFF4519 at 307.898 kb on + strand at 308.011 kb on - strand, within GFF4520 at 308.011 kb on - strand, within GFF4520 at 308.189 kb on + strand at 308.200 kb on - strand at 308.253 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 305,908 - GFF4516 0.86 -1.8 306,040 - +0.4 306,040 - -0.9 306,066 + +0.5 306,141 - -0.8 306,223 + +0.9 306,326 + GFF4517 0.24 +1.4 306,326 + GFF4517 0.24 +1.0 306,327 - GFF4517 0.24 -0.7 306,327 - GFF4517 0.24 +1.3 306,445 + GFF4517 0.45 -1.8 306,485 - GFF4517 0.52 +0.2 306,511 + GFF4517 0.57 -1.0 306,512 - GFF4517 0.57 -1.6 306,544 + GFF4517 0.63 -1.2 306,544 + GFF4517 0.63 +0.4 306,682 + GFF4517 0.87 -0.0 306,712 + +1.9 306,791 + +0.8 306,868 + GFF4518 0.15 -0.6 306,868 + GFF4518 0.15 +1.8 306,906 + GFF4518 0.20 -0.7 306,946 + GFF4518 0.25 -0.6 306,947 - GFF4518 0.26 -0.8 306,947 - GFF4518 0.26 -0.1 306,980 + GFF4518 0.30 -0.8 307,261 - GFF4518 0.66 -1.4 307,352 - GFF4518 0.78 +1.2 307,491 + +0.3 307,574 + -2.2 307,588 - -0.1 307,673 - GFF4519 0.30 -0.9 307,763 - GFF4519 0.60 -1.6 307,898 + +0.7 308,011 - GFF4520 0.44 -0.1 308,011 - GFF4520 0.44 -1.0 308,189 + -0.8 308,200 - -0.5 308,253 - -1.9
Or see this region's nucleotide sequence