Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4502 and GFF4503 overlap by 1 nucleotides GFF4503 and GFF4504 are separated by 2 nucleotides GFF4504 and GFF4505 are separated by 181 nucleotides
GFF4502 - Isochorismatase (EC 3.3.2.1) of siderophore biosynthesis, at 289,278 to 290,135
GFF4502
GFF4503 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EC 1.3.1.28) of siderophore biosynthesis, at 290,135 to 290,890
GFF4503
GFF4504 - Proofreading thioesterase in enterobactin biosynthesis EntH, at 290,893 to 291,306
GFF4504
GFF4505 - Carbon starvation protein A, at 291,488 to 293,593
GFF4505
Position (kb) 290
291
292 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 290.015 kb on - strand, within GFF4502 at 290.051 kb on - strand at 290.133 kb on + strand at 290.386 kb on + strand, within GFF4503 at 290.548 kb on + strand, within GFF4503 at 290.563 kb on + strand, within GFF4503 at 290.584 kb on + strand, within GFF4503 at 290.651 kb on - strand, within GFF4503 at 290.842 kb on + strand at 290.842 kb on + strand at 290.843 kb on - strand at 290.974 kb on + strand, within GFF4504 at 290.975 kb on - strand, within GFF4504 at 291.103 kb on + strand, within GFF4504 at 291.296 kb on + strand at 291.376 kb on - strand at 291.439 kb on + strand at 291.439 kb on + strand at 291.439 kb on + strand at 291.439 kb on + strand at 291.493 kb on + strand at 291.494 kb on - strand at 291.506 kb on + strand at 291.554 kb on + strand at 291.557 kb on - strand at 291.557 kb on - strand at 291.557 kb on - strand at 291.620 kb on + strand at 291.620 kb on + strand at 291.621 kb on - strand at 291.626 kb on - strand at 291.650 kb on + strand at 291.651 kb on - strand at 291.652 kb on + strand at 291.688 kb on + strand at 291.689 kb on - strand at 291.709 kb on + strand, within GFF4505 at 291.725 kb on + strand, within GFF4505 at 291.886 kb on + strand, within GFF4505 at 292.067 kb on + strand, within GFF4505 at 292.067 kb on + strand, within GFF4505 at 292.067 kb on + strand, within GFF4505 at 292.068 kb on - strand, within GFF4505 at 292.113 kb on - strand, within GFF4505 at 292.186 kb on + strand, within GFF4505 at 292.230 kb on - strand, within GFF4505 at 292.274 kb on + strand, within GFF4505
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 290,015 - GFF4502 0.86 -1.5 290,051 - +0.2 290,133 + -1.0 290,386 + GFF4503 0.33 -1.6 290,548 + GFF4503 0.55 -1.3 290,563 + GFF4503 0.57 -0.1 290,584 + GFF4503 0.59 -1.6 290,651 - GFF4503 0.68 +0.4 290,842 + +2.3 290,842 + +1.7 290,843 - +0.1 290,974 + GFF4504 0.20 -0.9 290,975 - GFF4504 0.20 -0.2 291,103 + GFF4504 0.51 -0.8 291,296 + -0.4 291,376 - +1.6 291,439 + +0.6 291,439 + +0.9 291,439 + -0.9 291,439 + +1.7 291,493 + +0.2 291,494 - +1.2 291,506 + -1.4 291,554 + -0.6 291,557 - -1.3 291,557 - -3.1 291,557 - -0.0 291,620 + +1.4 291,620 + -0.1 291,621 - +2.8 291,626 - -0.4 291,650 + -1.0 291,651 - -1.1 291,652 + -2.5 291,688 + +0.5 291,689 - +2.8 291,709 + GFF4505 0.10 -3.2 291,725 + GFF4505 0.11 +0.2 291,886 + GFF4505 0.19 -0.6 292,067 + GFF4505 0.27 -0.5 292,067 + GFF4505 0.27 -0.2 292,067 + GFF4505 0.27 +0.4 292,068 - GFF4505 0.28 -0.2 292,113 - GFF4505 0.30 +1.2 292,186 + GFF4505 0.33 -0.1 292,230 - GFF4505 0.35 +0.5 292,274 + GFF4505 0.37 +1.8
Or see this region's nucleotide sequence