Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4438 and GFF4439 overlap by 4 nucleotides GFF4439 and GFF4440 are separated by 117 nucleotides GFF4440 and GFF4441 are separated by 2 nucleotides GFF4441 and GFF4442 are separated by 172 nucleotides
GFF4438 - Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21), at 226,266 to 227,333
GFF4438
GFF4439 - Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21), at 227,330 to 227,839
GFF4439
GFF4440 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 227,957 to 228,679
GFF4440
GFF4441 - Peptidyl-prolyl cis-trans isomerase PpiB (EC 5.2.1.8), at 228,682 to 229,176
GFF4441
GFF4442 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 229,349 to 230,734
GFF4442
Position (kb) 227
228
229 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 226.977 kb on - strand, within GFF4438 at 227.527 kb on - strand, within GFF4439 at 227.723 kb on + strand, within GFF4439 at 227.724 kb on - strand, within GFF4439 at 227.724 kb on - strand, within GFF4439 at 227.894 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.230 kb on - strand at 229.230 kb on - strand at 229.248 kb on - strand at 229.297 kb on - strand at 229.297 kb on - strand at 229.297 kb on - strand at 229.303 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 226,977 - GFF4438 0.67 -1.4 227,527 - GFF4439 0.39 -2.0 227,723 + GFF4439 0.77 -0.7 227,724 - GFF4439 0.77 -0.9 227,724 - GFF4439 0.77 -0.4 227,894 - -1.8 229,228 - -3.2 229,228 - -0.1 229,228 - +0.6 229,228 - -0.9 229,228 - -0.2 229,228 - -0.7 229,228 - +0.8 229,230 - -1.3 229,230 - -3.9 229,248 - -1.1 229,297 - -1.5 229,297 - -0.2 229,297 - -1.8 229,303 + +0.1
Or see this region's nucleotide sequence