Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4434

Experiment: LB_plus_SM_buffer with P22_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4433 and GFF4434 are separated by 330 nucleotidesGFF4434 and GFF4435 are separated by 9 nucleotides GFF4433 - Ureidoglycolate dehydrogenase (EC 1.1.1.154), at 220,194 to 221,243 GFF4433 GFF4434 - Protein fdrA, at 221,574 to 223,238 GFF4434 GFF4435 - FIG074102: hypothetical protein, at 223,248 to 224,507 GFF4435 Position (kb) 221 222 223 224Strain fitness (log2 ratio) -2 -1 0 1 2at 220.593 kb on + strand, within GFF4433at 220.731 kb on - strand, within GFF4433at 220.775 kb on + strand, within GFF4433at 221.090 kb on - strand, within GFF4433at 221.140 kb on + strandat 222.189 kb on - strand, within GFF4434at 222.234 kb on - strand, within GFF4434at 222.515 kb on - strand, within GFF4434at 222.579 kb on + strand, within GFF4434at 222.717 kb on + strand, within GFF4434at 222.719 kb on + strand, within GFF4434at 222.762 kb on - strand, within GFF4434at 223.514 kb on + strand, within GFF4435at 223.560 kb on - strand, within GFF4435at 223.560 kb on - strand, within GFF4435at 223.723 kb on + strand, within GFF4435at 223.724 kb on - strand, within GFF4435at 224.030 kb on + strand, within GFF4435

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI
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220,593 + GFF4433 0.38 +1.7
220,731 - GFF4433 0.51 +0.0
220,775 + GFF4433 0.55 -0.6
221,090 - GFF4433 0.85 -1.9
221,140 + +0.3
222,189 - GFF4434 0.37 +1.7
222,234 - GFF4434 0.40 -0.1
222,515 - GFF4434 0.57 -1.1
222,579 + GFF4434 0.60 +1.5
222,717 + GFF4434 0.69 +0.6
222,719 + GFF4434 0.69 -1.2
222,762 - GFF4434 0.71 +1.0
223,514 + GFF4435 0.21 +0.9
223,560 - GFF4435 0.25 -1.7
223,560 - GFF4435 0.25 -0.2
223,723 + GFF4435 0.38 -1.0
223,724 - GFF4435 0.38 +2.4
224,030 + GFF4435 0.62 -1.0

Or see this region's nucleotide sequence