Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4429 and GFF4430 are separated by 22 nucleotides GFF4430 and GFF4431 are separated by 79 nucleotides GFF4431 and GFF4432 are separated by 10 nucleotides
GFF4429 - Xanthine permease, at 215,592 to 216,890
GFF4429
GFF4430 - Glycerate kinase (EC 2.7.1.31), at 216,913 to 218,061
GFF4430
GFF4431 - Ureidoglycine aminohydrolase, at 218,141 to 218,926
GFF4431
GFF4432 - Allantoate amidohydrolase (EC 3.5.3.9), at 218,937 to 220,172
GFF4432
Position (kb) 216
217
218
219 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 215.954 kb on - strand, within GFF4429 at 216.077 kb on + strand, within GFF4429 at 216.168 kb on - strand, within GFF4429 at 216.245 kb on - strand, within GFF4429 at 216.309 kb on - strand, within GFF4429 at 216.380 kb on + strand, within GFF4429 at 216.496 kb on + strand, within GFF4429 at 216.497 kb on - strand, within GFF4429 at 216.586 kb on + strand, within GFF4429 at 216.720 kb on + strand, within GFF4429 at 216.819 kb on - strand at 216.982 kb on - strand at 217.001 kb on + strand at 217.002 kb on - strand at 217.173 kb on + strand, within GFF4430 at 217.299 kb on + strand, within GFF4430 at 217.497 kb on + strand, within GFF4430 at 217.593 kb on + strand, within GFF4430 at 217.597 kb on + strand, within GFF4430 at 218.013 kb on + strand at 218.105 kb on - strand at 218.176 kb on - strand at 218.181 kb on + strand at 218.248 kb on - strand, within GFF4431 at 218.314 kb on - strand, within GFF4431 at 218.347 kb on - strand, within GFF4431 at 218.381 kb on - strand, within GFF4431 at 218.386 kb on + strand, within GFF4431 at 218.387 kb on - strand, within GFF4431 at 218.387 kb on - strand, within GFF4431 at 218.452 kb on - strand, within GFF4431 at 218.519 kb on - strand, within GFF4431 at 218.519 kb on - strand, within GFF4431 at 218.519 kb on - strand, within GFF4431 at 218.531 kb on - strand, within GFF4431 at 218.541 kb on - strand, within GFF4431 at 218.598 kb on + strand, within GFF4431 at 218.645 kb on - strand, within GFF4431 at 218.729 kb on + strand, within GFF4431 at 218.729 kb on + strand, within GFF4431 at 219.002 kb on + strand at 219.003 kb on - strand at 219.003 kb on - strand at 219.012 kb on - strand at 219.036 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 215,954 - GFF4429 0.28 -0.4 216,077 + GFF4429 0.37 -0.4 216,168 - GFF4429 0.44 +0.3 216,245 - GFF4429 0.50 -0.7 216,309 - GFF4429 0.55 -1.2 216,380 + GFF4429 0.61 -0.1 216,496 + GFF4429 0.70 +0.4 216,497 - GFF4429 0.70 +1.6 216,586 + GFF4429 0.77 +0.7 216,720 + GFF4429 0.87 +0.2 216,819 - -1.7 216,982 - -0.4 217,001 + -1.3 217,002 - -0.1 217,173 + GFF4430 0.23 +0.4 217,299 + GFF4430 0.34 -2.4 217,497 + GFF4430 0.51 -1.7 217,593 + GFF4430 0.59 -2.1 217,597 + GFF4430 0.60 -0.0 218,013 + -0.0 218,105 - -2.3 218,176 - +0.9 218,181 + -3.6 218,248 - GFF4431 0.14 +1.1 218,314 - GFF4431 0.22 +0.9 218,347 - GFF4431 0.26 -1.3 218,381 - GFF4431 0.31 -1.2 218,386 + GFF4431 0.31 -1.6 218,387 - GFF4431 0.31 -3.1 218,387 - GFF4431 0.31 +0.0 218,452 - GFF4431 0.40 -1.0 218,519 - GFF4431 0.48 -1.2 218,519 - GFF4431 0.48 +0.7 218,519 - GFF4431 0.48 +0.8 218,531 - GFF4431 0.50 -1.0 218,541 - GFF4431 0.51 -0.1 218,598 + GFF4431 0.58 -2.5 218,645 - GFF4431 0.64 +0.7 218,729 + GFF4431 0.75 -2.0 218,729 + GFF4431 0.75 -0.5 219,002 + +0.4 219,003 - +0.1 219,003 - -3.0 219,012 - +1.0 219,036 - -1.7
Or see this region's nucleotide sequence