Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4423 and GFF4424 are separated by 12 nucleotides GFF4424 and GFF4425 are separated by 100 nucleotides GFF4425 and GFF4426 are separated by 65 nucleotides
GFF4423 - Glyoxylate carboligase (EC 4.1.1.47), at 207,681 to 209,462
GFF4423
GFF4424 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 209,475 to 210,251
GFF4424
GFF4425 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 210,352 to 211,230
GFF4425
GFF4426 - Probable metabolite transport protein, at 211,296 to 212,543
GFF4426
Position (kb) 210
211
212 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 209.454 kb on + strand at 209.898 kb on - strand, within GFF4424 at 209.988 kb on - strand, within GFF4424 at 210.008 kb on + strand, within GFF4424 at 210.009 kb on - strand, within GFF4424 at 210.195 kb on + strand at 210.337 kb on + strand at 210.691 kb on + strand, within GFF4425 at 210.750 kb on + strand, within GFF4425 at 210.786 kb on + strand, within GFF4425 at 211.190 kb on + strand at 211.219 kb on - strand at 211.283 kb on - strand at 211.380 kb on + strand at 211.435 kb on + strand, within GFF4426 at 211.436 kb on - strand, within GFF4426 at 211.614 kb on + strand, within GFF4426 at 211.615 kb on - strand, within GFF4426 at 211.616 kb on + strand, within GFF4426 at 211.616 kb on + strand, within GFF4426 at 211.818 kb on + strand, within GFF4426 at 211.818 kb on + strand, within GFF4426 at 211.840 kb on + strand, within GFF4426 at 211.841 kb on - strand, within GFF4426 at 212.008 kb on - strand, within GFF4426 at 212.017 kb on + strand, within GFF4426 at 212.037 kb on + strand, within GFF4426 at 212.171 kb on + strand, within GFF4426
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 209,454 + -2.1 209,898 - GFF4424 0.54 +0.5 209,988 - GFF4424 0.66 -1.9 210,008 + GFF4424 0.69 -1.2 210,009 - GFF4424 0.69 -1.1 210,195 + -0.2 210,337 + -0.3 210,691 + GFF4425 0.39 -1.9 210,750 + GFF4425 0.45 -2.1 210,786 + GFF4425 0.49 -1.5 211,190 + +0.4 211,219 - -2.2 211,283 - -1.6 211,380 + -0.8 211,435 + GFF4426 0.11 -2.7 211,436 - GFF4426 0.11 -0.3 211,614 + GFF4426 0.25 -1.4 211,615 - GFF4426 0.26 -2.2 211,616 + GFF4426 0.26 -0.6 211,616 + GFF4426 0.26 -1.0 211,818 + GFF4426 0.42 -0.3 211,818 + GFF4426 0.42 +0.6 211,840 + GFF4426 0.44 -3.0 211,841 - GFF4426 0.44 -2.5 212,008 - GFF4426 0.57 +0.6 212,017 + GFF4426 0.58 -1.7 212,037 + GFF4426 0.59 -3.5 212,171 + GFF4426 0.70 -1.5
Or see this region's nucleotide sequence