Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4402 and GFF4403 are separated by 85 nucleotides GFF4403 and GFF4404 are separated by 153 nucleotides
GFF4402 - FIG00921183: possible ligase, at 186,298 to 187,092
GFF4402
GFF4403 - Probable secreted protein, at 187,178 to 188,005
GFF4403
GFF4404 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 188,159 to 190,660
GFF4404
Position (kb)
187
188
189 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 186.249 kb on - strand at 186.362 kb on + strand at 186.425 kb on - strand, within GFF4402 at 186.444 kb on + strand, within GFF4402 at 186.659 kb on - strand, within GFF4402 at 186.661 kb on + strand, within GFF4402 at 186.662 kb on - strand, within GFF4402 at 186.668 kb on + strand, within GFF4402 at 186.721 kb on + strand, within GFF4402 at 186.729 kb on + strand, within GFF4402 at 186.730 kb on - strand, within GFF4402 at 186.730 kb on - strand, within GFF4402 at 186.730 kb on - strand, within GFF4402 at 186.733 kb on + strand, within GFF4402 at 186.734 kb on - strand, within GFF4402 at 186.734 kb on - strand, within GFF4402 at 186.774 kb on + strand, within GFF4402 at 187.023 kb on + strand at 187.082 kb on - strand at 187.234 kb on + strand at 187.263 kb on - strand, within GFF4403 at 187.340 kb on + strand, within GFF4403 at 187.341 kb on - strand, within GFF4403 at 187.469 kb on + strand, within GFF4403 at 187.573 kb on - strand, within GFF4403 at 187.716 kb on - strand, within GFF4403 at 188.240 kb on + strand at 188.313 kb on + strand at 188.345 kb on - strand at 188.398 kb on + strand at 188.654 kb on + strand, within GFF4404 at 188.698 kb on + strand, within GFF4404 at 188.938 kb on + strand, within GFF4404
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 186,249 - -2.0 186,362 + -2.0 186,425 - GFF4402 0.16 -0.8 186,444 + GFF4402 0.18 -0.3 186,659 - GFF4402 0.45 -1.8 186,661 + GFF4402 0.46 -1.3 186,662 - GFF4402 0.46 -1.2 186,668 + GFF4402 0.47 -1.2 186,721 + GFF4402 0.53 +0.7 186,729 + GFF4402 0.54 -0.7 186,730 - GFF4402 0.54 -0.3 186,730 - GFF4402 0.54 +2.4 186,730 - GFF4402 0.54 -3.3 186,733 + GFF4402 0.55 -3.7 186,734 - GFF4402 0.55 -0.0 186,734 - GFF4402 0.55 +0.3 186,774 + GFF4402 0.60 +0.2 187,023 + -0.3 187,082 - -1.0 187,234 + -3.1 187,263 - GFF4403 0.10 -1.1 187,340 + GFF4403 0.20 +0.1 187,341 - GFF4403 0.20 -1.7 187,469 + GFF4403 0.35 -0.0 187,573 - GFF4403 0.48 -0.4 187,716 - GFF4403 0.65 -1.7 188,240 + -0.6 188,313 + -1.3 188,345 - -1.3 188,398 + +0.1 188,654 + GFF4404 0.20 -1.0 188,698 + GFF4404 0.22 -1.3 188,938 + GFF4404 0.31 -1.8
Or see this region's nucleotide sequence