Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4397 and GFF4398 are separated by 77 nucleotides GFF4398 and GFF4399 are separated by 11 nucleotides GFF4399 and GFF4400 are separated by 172 nucleotides
GFF4397 - POTASSIUM/PROTON ANTIPORTER ROSB, at 180,564 to 182,240
GFF4397
GFF4398 - hypothetical protein, at 182,318 to 182,443
GFF4398
GFF4399 - Fosmidomycin resistance protein, at 182,455 to 183,675
GFF4399
GFF4400 - 'UDP-sugar hydrolase (EC 3.6.1.45); 5'-nucleotidase (EC 3.1.3.5)' transl_table=11, at 183,848 to 185,500
GFF4400
Position (kb) 182
183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 181.458 kb on - strand, within GFF4397 at 181.458 kb on - strand, within GFF4397 at 181.659 kb on - strand, within GFF4397 at 181.659 kb on - strand, within GFF4397 at 181.899 kb on + strand, within GFF4397 at 181.899 kb on + strand, within GFF4397 at 182.463 kb on + strand at 182.660 kb on + strand, within GFF4399 at 182.661 kb on - strand, within GFF4399 at 182.778 kb on + strand, within GFF4399 at 182.918 kb on + strand, within GFF4399 at 182.951 kb on + strand, within GFF4399 at 182.980 kb on + strand, within GFF4399 at 183.006 kb on + strand, within GFF4399 at 183.329 kb on - strand, within GFF4399 at 183.379 kb on - strand, within GFF4399 at 183.420 kb on + strand, within GFF4399 at 183.421 kb on - strand, within GFF4399 at 183.561 kb on + strand at 183.561 kb on + strand at 183.613 kb on - strand at 183.729 kb on + strand at 183.785 kb on + strand at 183.924 kb on - strand at 184.007 kb on + strand at 184.103 kb on - strand, within GFF4400 at 184.103 kb on - strand, within GFF4400 at 184.193 kb on - strand, within GFF4400 at 184.221 kb on + strand, within GFF4400 at 184.270 kb on + strand, within GFF4400 at 184.271 kb on - strand, within GFF4400 at 184.380 kb on + strand, within GFF4400 at 184.459 kb on + strand, within GFF4400 at 184.652 kb on + strand, within GFF4400 at 184.652 kb on + strand, within GFF4400 at 184.657 kb on - strand, within GFF4400
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 181,458 - GFF4397 0.53 -0.0 181,458 - GFF4397 0.53 -3.0 181,659 - GFF4397 0.65 +1.0 181,659 - GFF4397 0.65 -2.3 181,899 + GFF4397 0.80 -2.9 181,899 + GFF4397 0.80 -0.7 182,463 + -0.9 182,660 + GFF4399 0.17 -0.3 182,661 - GFF4399 0.17 -3.0 182,778 + GFF4399 0.26 -2.9 182,918 + GFF4399 0.38 -2.7 182,951 + GFF4399 0.41 -2.8 182,980 + GFF4399 0.43 -1.9 183,006 + GFF4399 0.45 -2.8 183,329 - GFF4399 0.72 -0.7 183,379 - GFF4399 0.76 -0.7 183,420 + GFF4399 0.79 +0.7 183,421 - GFF4399 0.79 -1.3 183,561 + +0.9 183,561 + -0.2 183,613 - -0.2 183,729 + -0.5 183,785 + -1.2 183,924 - +0.1 184,007 + -0.7 184,103 - GFF4400 0.15 -2.1 184,103 - GFF4400 0.15 -2.5 184,193 - GFF4400 0.21 -2.2 184,221 + GFF4400 0.23 -0.8 184,270 + GFF4400 0.26 -1.4 184,271 - GFF4400 0.26 +0.2 184,380 + GFF4400 0.32 -3.3 184,459 + GFF4400 0.37 -0.0 184,652 + GFF4400 0.49 +0.6 184,652 + GFF4400 0.49 -1.2 184,657 - GFF4400 0.49 +0.7
Or see this region's nucleotide sequence