Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4393 and GFF4394 are separated by 9 nucleotides GFF4394 and GFF4395 overlap by 4 nucleotides GFF4395 and GFF4396 are separated by 152 nucleotides
GFF4393 - Adenylate kinase (EC 2.7.4.3), at 176,978 to 177,118
GFF4393
GFF4394 - Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1), at 177,128 to 178,090
GFF4394
GFF4395 - Acetyl esterase (EC 3.1.1.-), at 178,087 to 179,058
GFF4395
GFF4396 - Inosine-guanosine kinase (EC 2.7.1.73), at 179,211 to 180,515
GFF4396
Position (kb) 178
179
180 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 177.116 kb on + strand at 178.326 kb on + strand, within GFF4395 at 178.330 kb on - strand, within GFF4395 at 178.518 kb on + strand, within GFF4395 at 178.519 kb on - strand, within GFF4395 at 178.519 kb on - strand, within GFF4395 at 178.519 kb on - strand, within GFF4395 at 178.629 kb on - strand, within GFF4395 at 178.691 kb on + strand, within GFF4395 at 178.797 kb on - strand, within GFF4395 at 178.797 kb on - strand, within GFF4395 at 178.797 kb on - strand, within GFF4395 at 178.798 kb on + strand, within GFF4395 at 178.799 kb on - strand, within GFF4395 at 178.799 kb on - strand, within GFF4395 at 178.843 kb on + strand, within GFF4395 at 178.843 kb on + strand, within GFF4395 at 178.844 kb on - strand, within GFF4395 at 178.929 kb on - strand, within GFF4395 at 178.932 kb on - strand, within GFF4395 at 178.997 kb on - strand at 179.244 kb on + strand at 179.413 kb on + strand, within GFF4396 at 179.595 kb on + strand, within GFF4396 at 179.607 kb on - strand, within GFF4396 at 179.644 kb on + strand, within GFF4396 at 179.697 kb on - strand, within GFF4396 at 179.720 kb on + strand, within GFF4396 at 179.794 kb on - strand, within GFF4396 at 179.808 kb on - strand, within GFF4396 at 180.055 kb on + strand, within GFF4396
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 177,116 + -2.6 178,326 + GFF4395 0.25 -0.4 178,330 - GFF4395 0.25 -2.6 178,518 + GFF4395 0.44 -1.2 178,519 - GFF4395 0.44 -3.0 178,519 - GFF4395 0.44 -2.2 178,519 - GFF4395 0.44 -0.8 178,629 - GFF4395 0.56 -1.5 178,691 + GFF4395 0.62 -1.3 178,797 - GFF4395 0.73 -1.8 178,797 - GFF4395 0.73 -1.6 178,797 - GFF4395 0.73 +0.2 178,798 + GFF4395 0.73 +0.1 178,799 - GFF4395 0.73 -0.7 178,799 - GFF4395 0.73 -2.4 178,843 + GFF4395 0.78 -3.1 178,843 + GFF4395 0.78 -1.5 178,844 - GFF4395 0.78 +1.0 178,929 - GFF4395 0.87 -0.6 178,932 - GFF4395 0.87 -2.6 178,997 - +0.5 179,244 + -0.8 179,413 + GFF4396 0.15 -2.2 179,595 + GFF4396 0.29 -1.8 179,607 - GFF4396 0.30 -0.6 179,644 + GFF4396 0.33 -1.7 179,697 - GFF4396 0.37 +2.3 179,720 + GFF4396 0.39 -0.1 179,794 - GFF4396 0.45 -1.1 179,808 - GFF4396 0.46 +0.0 180,055 + GFF4396 0.65 -0.8
Or see this region's nucleotide sequence