Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF426 and GFF427 overlap by 7 nucleotides GFF427 and GFF428 overlap by 8 nucleotides GFF428 and GFF429 overlap by 4 nucleotides
GFF426 - L-ribulose-5-phosphate 4-epimerase (EC 5.1.3.4), at 148,418 to 149,113
GFF426
GFF427 - L-xylulose 5-phosphate 3-epimerase (EC 5.1.3.-) homolog, at 149,107 to 149,967
GFF427
GFF428 - 3-keto-L-gulonate 6-phosphate decarboxylase homolog, at 149,960 to 150,622
GFF428
GFF429 - L-xylulose/3-keto-L-gulonate kinase (EC 2.7.1.-), at 150,619 to 152,115
GFF429
Position (kb) 149
150
151 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 149.109 kb on - strand at 149.427 kb on - strand, within GFF427 at 149.607 kb on - strand, within GFF427 at 149.607 kb on - strand, within GFF427 at 149.610 kb on - strand, within GFF427 at 149.950 kb on + strand at 149.951 kb on - strand at 150.219 kb on + strand, within GFF428 at 150.367 kb on + strand, within GFF428 at 150.368 kb on - strand, within GFF428 at 150.609 kb on - strand at 150.658 kb on + strand at 150.659 kb on - strand at 150.659 kb on - strand at 150.785 kb on - strand, within GFF429 at 150.999 kb on - strand, within GFF429 at 150.999 kb on - strand, within GFF429 at 151.230 kb on + strand, within GFF429 at 151.230 kb on + strand, within GFF429 at 151.232 kb on + strand, within GFF429 at 151.233 kb on - strand, within GFF429 at 151.260 kb on - strand, within GFF429 at 151.265 kb on + strand, within GFF429 at 151.265 kb on + strand, within GFF429 at 151.265 kb on + strand, within GFF429 at 151.544 kb on + strand, within GFF429 at 151.566 kb on - strand, within GFF429
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 149,109 - -1.4 149,427 - GFF427 0.37 +1.2 149,607 - GFF427 0.58 -0.6 149,607 - GFF427 0.58 +0.8 149,610 - GFF427 0.58 -1.6 149,950 + +0.9 149,951 - +0.6 150,219 + GFF428 0.39 -3.3 150,367 + GFF428 0.61 +0.9 150,368 - GFF428 0.62 -0.3 150,609 - -0.8 150,658 + +0.4 150,659 - +2.0 150,659 - -0.2 150,785 - GFF429 0.11 -0.4 150,999 - GFF429 0.25 -0.2 150,999 - GFF429 0.25 +1.9 151,230 + GFF429 0.41 -1.2 151,230 + GFF429 0.41 +0.4 151,232 + GFF429 0.41 -0.8 151,233 - GFF429 0.41 -1.6 151,260 - GFF429 0.43 -1.7 151,265 + GFF429 0.43 -2.6 151,265 + GFF429 0.43 +0.7 151,265 + GFF429 0.43 +0.1 151,544 + GFF429 0.62 -0.3 151,566 - GFF429 0.63 +2.5
Or see this region's nucleotide sequence