Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4053 and GFF4054 are separated by 379 nucleotides GFF4054 and GFF4055 are separated by 99 nucleotides GFF4055 and GFF4056 are separated by 23 nucleotides
GFF4053 - FIG074102: hypothetical protein, at 15,334 to 16,224
GFF4053
GFF4054 - ClpB protein, at 16,604 to 19,243
GFF4054
GFF4055 - Uncharacterized protein ImpB, at 19,343 to 19,885
GFF4055
GFF4056 - Uncharacterized protein ImpC, at 19,909 to 21,417
GFF4056
Position (kb) 16
17
18
19
20 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 15.627 kb on - strand, within GFF4053 at 15.627 kb on - strand, within GFF4053 at 15.726 kb on - strand, within GFF4053 at 15.841 kb on - strand, within GFF4053 at 15.970 kb on - strand, within GFF4053 at 15.990 kb on + strand, within GFF4053 at 16.151 kb on - strand at 16.157 kb on + strand at 16.158 kb on - strand at 16.158 kb on - strand at 16.695 kb on + strand at 17.006 kb on - strand, within GFF4054 at 17.303 kb on - strand, within GFF4054 at 17.406 kb on + strand, within GFF4054 at 17.407 kb on - strand, within GFF4054 at 17.658 kb on - strand, within GFF4054 at 17.668 kb on + strand, within GFF4054 at 18.118 kb on + strand, within GFF4054 at 18.119 kb on - strand, within GFF4054 at 18.119 kb on - strand, within GFF4054 at 18.119 kb on - strand, within GFF4054 at 18.119 kb on - strand, within GFF4054 at 18.446 kb on - strand, within GFF4054 at 18.446 kb on - strand, within GFF4054 at 18.541 kb on + strand, within GFF4054 at 18.755 kb on - strand, within GFF4054 at 18.784 kb on - strand, within GFF4054 at 18.787 kb on - strand, within GFF4054 at 18.787 kb on - strand, within GFF4054 at 18.843 kb on + strand, within GFF4054 at 18.844 kb on - strand, within GFF4054 at 18.865 kb on + strand, within GFF4054 at 18.866 kb on - strand, within GFF4054 at 18.938 kb on + strand, within GFF4054 at 18.939 kb on - strand, within GFF4054 at 19.037 kb on + strand at 19.037 kb on + strand at 19.037 kb on + strand at 19.037 kb on + strand at 19.040 kb on - strand at 19.060 kb on + strand at 19.060 kb on + strand at 19.061 kb on - strand at 19.061 kb on - strand at 19.652 kb on - strand, within GFF4055 at 19.746 kb on - strand, within GFF4055 at 20.102 kb on - strand, within GFF4056 at 20.227 kb on + strand, within GFF4056
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 15,627 - GFF4053 0.33 -3.6 15,627 - GFF4053 0.33 -0.2 15,726 - GFF4053 0.44 -0.2 15,841 - GFF4053 0.57 +0.5 15,970 - GFF4053 0.71 +0.8 15,990 + GFF4053 0.74 -0.4 16,151 - -0.1 16,157 + +0.5 16,158 - -0.3 16,158 - +0.3 16,695 + -0.4 17,006 - GFF4054 0.15 -0.2 17,303 - GFF4054 0.26 -1.4 17,406 + GFF4054 0.30 -0.5 17,407 - GFF4054 0.30 -1.1 17,658 - GFF4054 0.40 +0.2 17,668 + GFF4054 0.40 +1.1 18,118 + GFF4054 0.57 -0.2 18,119 - GFF4054 0.57 -1.0 18,119 - GFF4054 0.57 +0.8 18,119 - GFF4054 0.57 -0.2 18,119 - GFF4054 0.57 +0.0 18,446 - GFF4054 0.70 -0.6 18,446 - GFF4054 0.70 -0.2 18,541 + GFF4054 0.73 +0.6 18,755 - GFF4054 0.81 -1.1 18,784 - GFF4054 0.83 -1.5 18,787 - GFF4054 0.83 -2.0 18,787 - GFF4054 0.83 +0.4 18,843 + GFF4054 0.85 +1.2 18,844 - GFF4054 0.85 -0.0 18,865 + GFF4054 0.86 -1.0 18,866 - GFF4054 0.86 +0.1 18,938 + GFF4054 0.88 -0.6 18,939 - GFF4054 0.88 +0.6 19,037 + +0.5 19,037 + -0.4 19,037 + +0.3 19,037 + +0.7 19,040 - +1.4 19,060 + +1.8 19,060 + -0.0 19,061 - -1.2 19,061 - -2.5 19,652 - GFF4055 0.57 +0.4 19,746 - GFF4055 0.74 +2.5 20,102 - GFF4056 0.13 -0.5 20,227 + GFF4056 0.21 -1.2
Or see this region's nucleotide sequence