Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3978 and GFF3979 are separated by 56 nucleotides GFF3979 and GFF3980 are separated by 209 nucleotides GFF3980 and GFF3981 overlap by 23 nucleotides
GFF3978 - Putative periplasmic protein, at 254,451 to 256,181
GFF3978
GFF3979 - FIG01057005: hypothetical protein, at 256,238 to 258,406
GFF3979
GFF3980 - Transposase, at 258,616 to 258,771
GFF3980
GFF3981 - FIG01048908: hypothetical protein, at 258,749 to 259,447
GFF3981
Position (kb) 256
257
258
259 Strain fitness (log2 ratio) -1
0
1
2
3 at 255.440 kb on + strand, within GFF3978 at 255.454 kb on + strand, within GFF3978 at 255.491 kb on - strand, within GFF3978 at 255.518 kb on + strand, within GFF3978 at 255.627 kb on - strand, within GFF3978 at 255.802 kb on + strand, within GFF3978 at 256.235 kb on + strand at 256.239 kb on + strand at 256.362 kb on + strand at 256.378 kb on + strand at 256.379 kb on - strand at 256.429 kb on - strand at 256.530 kb on + strand, within GFF3979 at 256.578 kb on + strand, within GFF3979 at 256.579 kb on - strand, within GFF3979 at 256.677 kb on + strand, within GFF3979 at 256.693 kb on - strand, within GFF3979 at 256.749 kb on - strand, within GFF3979 at 256.794 kb on - strand, within GFF3979 at 256.859 kb on + strand, within GFF3979 at 256.860 kb on - strand, within GFF3979 at 256.883 kb on - strand, within GFF3979 at 257.007 kb on + strand, within GFF3979 at 257.008 kb on - strand, within GFF3979 at 257.039 kb on - strand, within GFF3979 at 257.066 kb on - strand, within GFF3979 at 257.939 kb on + strand, within GFF3979 at 258.842 kb on - strand, within GFF3981 at 258.865 kb on + strand, within GFF3981 at 259.089 kb on + strand, within GFF3981 at 259.089 kb on + strand, within GFF3981 at 259.277 kb on - strand, within GFF3981 at 259.278 kb on + strand, within GFF3981 at 259.279 kb on - strand, within GFF3981 at 259.279 kb on - strand, within GFF3981 at 259.300 kb on + strand, within GFF3981 at 259.340 kb on + strand, within GFF3981
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 255,440 + GFF3978 0.57 -0.0 255,454 + GFF3978 0.58 +0.1 255,491 - GFF3978 0.60 +0.9 255,518 + GFF3978 0.62 -0.1 255,627 - GFF3978 0.68 -0.2 255,802 + GFF3978 0.78 +0.6 256,235 + -0.2 256,239 + -1.5 256,362 + +0.1 256,378 + +0.0 256,379 - -0.4 256,429 - -0.5 256,530 + GFF3979 0.13 -0.5 256,578 + GFF3979 0.16 +0.6 256,579 - GFF3979 0.16 +0.6 256,677 + GFF3979 0.20 +1.6 256,693 - GFF3979 0.21 +0.7 256,749 - GFF3979 0.24 +1.0 256,794 - GFF3979 0.26 -0.9 256,859 + GFF3979 0.29 +0.1 256,860 - GFF3979 0.29 +0.1 256,883 - GFF3979 0.30 +0.7 257,007 + GFF3979 0.35 +3.1 257,008 - GFF3979 0.36 +0.7 257,039 - GFF3979 0.37 -0.5 257,066 - GFF3979 0.38 +1.8 257,939 + GFF3979 0.78 -0.5 258,842 - GFF3981 0.13 +0.9 258,865 + GFF3981 0.17 -1.5 259,089 + GFF3981 0.49 +0.2 259,089 + GFF3981 0.49 +0.9 259,277 - GFF3981 0.76 +0.8 259,278 + GFF3981 0.76 +0.0 259,279 - GFF3981 0.76 +0.3 259,279 - GFF3981 0.76 +0.8 259,300 + GFF3981 0.79 -0.8 259,340 + GFF3981 0.85 -0.2
Or see this region's nucleotide sequence