Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3553 and GFF3554 are separated by 264 nucleotides GFF3554 and GFF3555 overlap by 4 nucleotides GFF3555 and GFF3556 are separated by 98 nucleotides GFF3556 and GFF3557 are separated by 287 nucleotides
GFF3553 - Putative membrane protein, at 307,174 to 308,292
GFF3553
GFF3554 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 308,557 to 311,613
GFF3554
GFF3555 - 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (EC 3.1.2.28) in menaquinone biosynthesis, at 311,610 to 312,020
GFF3555
GFF3556 - FIG00639040: hypothetical protein, at 312,119 to 312,307
GFF3556
GFF3557 - L-cystine uptake protein TcyP, at 312,595 to 313,941
GFF3557
Position (kb) 308
309
310
311
312 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 307.622 kb on - strand, within GFF3553 at 307.730 kb on - strand, within GFF3553 at 307.731 kb on + strand, within GFF3553 at 307.732 kb on - strand, within GFF3553 at 307.857 kb on + strand, within GFF3553 at 307.859 kb on + strand, within GFF3553 at 307.860 kb on - strand, within GFF3553 at 307.882 kb on - strand, within GFF3553 at 307.909 kb on - strand, within GFF3553 at 307.950 kb on + strand, within GFF3553 at 307.951 kb on - strand, within GFF3553 at 308.190 kb on + strand at 308.200 kb on + strand at 308.265 kb on + strand at 308.266 kb on - strand at 308.406 kb on + strand at 308.440 kb on - strand at 308.533 kb on - strand at 308.533 kb on - strand at 308.553 kb on + strand at 308.659 kb on + strand at 308.660 kb on - strand at 308.841 kb on - strand at 308.872 kb on + strand, within GFF3554 at 308.875 kb on - strand, within GFF3554 at 308.936 kb on + strand, within GFF3554 at 309.034 kb on - strand, within GFF3554 at 309.196 kb on + strand, within GFF3554 at 309.197 kb on - strand, within GFF3554 at 309.281 kb on - strand, within GFF3554 at 309.287 kb on + strand, within GFF3554 at 309.387 kb on + strand, within GFF3554 at 309.468 kb on + strand, within GFF3554 at 309.537 kb on + strand, within GFF3554 at 309.747 kb on + strand, within GFF3554 at 309.961 kb on - strand, within GFF3554 at 310.158 kb on + strand, within GFF3554 at 310.290 kb on + strand, within GFF3554 at 310.291 kb on - strand, within GFF3554 at 310.311 kb on + strand, within GFF3554 at 310.811 kb on + strand, within GFF3554 at 310.869 kb on + strand, within GFF3554 at 311.053 kb on - strand, within GFF3554 at 311.053 kb on - strand, within GFF3554 at 311.055 kb on + strand, within GFF3554 at 311.474 kb on + strand at 311.474 kb on + strand at 311.475 kb on - strand at 311.604 kb on + strand at 311.607 kb on - strand at 311.607 kb on - strand at 311.821 kb on - strand, within GFF3555 at 311.956 kb on + strand, within GFF3555 at 312.032 kb on + strand at 312.047 kb on + strand at 312.048 kb on - strand at 312.084 kb on + strand at 312.085 kb on - strand at 312.086 kb on + strand at 312.087 kb on - strand at 312.106 kb on - strand at 312.106 kb on - strand at 312.118 kb on + strand at 312.118 kb on + strand at 312.119 kb on - strand at 312.119 kb on - strand at 312.308 kb on + strand at 312.309 kb on - strand at 312.309 kb on - strand at 312.550 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 307,622 - GFF3553 0.40 +0.1 307,730 - GFF3553 0.50 +1.1 307,731 + GFF3553 0.50 +1.3 307,732 - GFF3553 0.50 +0.1 307,857 + GFF3553 0.61 +0.1 307,859 + GFF3553 0.61 +0.6 307,860 - GFF3553 0.61 -0.7 307,882 - GFF3553 0.63 -3.1 307,909 - GFF3553 0.66 -2.1 307,950 + GFF3553 0.69 +0.3 307,951 - GFF3553 0.69 -3.0 308,190 + +1.6 308,200 + +0.3 308,265 + -2.1 308,266 - +0.4 308,406 + -0.5 308,440 - +0.6 308,533 - -0.9 308,533 - -0.4 308,553 + -0.1 308,659 + -3.8 308,660 - +0.2 308,841 - -1.2 308,872 + GFF3554 0.10 +1.2 308,875 - GFF3554 0.10 +0.4 308,936 + GFF3554 0.12 -0.4 309,034 - GFF3554 0.16 +0.8 309,196 + GFF3554 0.21 +0.3 309,197 - GFF3554 0.21 +1.8 309,281 - GFF3554 0.24 -0.5 309,287 + GFF3554 0.24 -0.4 309,387 + GFF3554 0.27 +0.7 309,468 + GFF3554 0.30 -0.5 309,537 + GFF3554 0.32 -1.5 309,747 + GFF3554 0.39 -0.5 309,961 - GFF3554 0.46 +0.5 310,158 + GFF3554 0.52 -0.1 310,290 + GFF3554 0.57 +0.6 310,291 - GFF3554 0.57 +1.8 310,311 + GFF3554 0.57 +0.5 310,811 + GFF3554 0.74 +0.9 310,869 + GFF3554 0.76 +0.2 311,053 - GFF3554 0.82 +0.5 311,053 - GFF3554 0.82 -2.6 311,055 + GFF3554 0.82 +0.8 311,474 + -0.7 311,474 + -0.9 311,475 - +0.7 311,604 + +0.1 311,607 - -1.5 311,607 - -0.4 311,821 - GFF3555 0.51 +0.1 311,956 + GFF3555 0.84 -1.9 312,032 + -0.2 312,047 + +1.0 312,048 - +0.5 312,084 + -1.6 312,085 - +1.4 312,086 + -0.4 312,087 - +0.9 312,106 - +0.7 312,106 - -1.2 312,118 + -1.1 312,118 + -0.1 312,119 - +1.9 312,119 - +1.3 312,308 + +0.8 312,309 - +1.2 312,309 - +0.4 312,550 + +0.3
Or see this region's nucleotide sequence