Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3465 and GFF3466 are separated by 100 nucleotides GFF3466 and GFF3467 overlap by 21 nucleotides GFF3467 and GFF3468 are separated by 1 nucleotides GFF3468 and GFF3469 are separated by 30 nucleotides GFF3469 and GFF3470 overlap by 4 nucleotides
GFF3465 - hypothetical protein, at 216,955 to 218,139
GFF3465
GFF3466 - Transcriptional regulator, AraC family, at 218,240 to 219,034
GFF3466
GFF3467 - Putative inner membrane protein, at 219,014 to 219,460
GFF3467
GFF3468 - hypothetical protein, at 219,462 to 219,599
GFF3468
GFF3469 - hypothetical protein, at 219,630 to 219,773
GFF3469
GFF3470 - Uncharacterized protein YeaK, at 219,770 to 220,297
GFF3470
Position (kb) 218
219
220 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 217.429 kb on + strand, within GFF3465 at 217.548 kb on + strand, within GFF3465 at 217.548 kb on + strand, within GFF3465 at 218.068 kb on - strand at 218.467 kb on + strand, within GFF3466 at 218.762 kb on - strand, within GFF3466 at 218.905 kb on + strand, within GFF3466 at 218.905 kb on + strand, within GFF3466 at 218.905 kb on + strand, within GFF3466 at 218.905 kb on + strand, within GFF3466 at 218.906 kb on - strand, within GFF3466 at 219.033 kb on - strand at 219.151 kb on + strand, within GFF3467 at 219.365 kb on - strand, within GFF3467 at 219.618 kb on + strand at 219.665 kb on + strand, within GFF3469 at 219.666 kb on - strand, within GFF3469 at 219.666 kb on - strand, within GFF3469 at 219.666 kb on - strand, within GFF3469 at 219.702 kb on - strand, within GFF3469 at 219.834 kb on + strand, within GFF3470 at 219.851 kb on - strand, within GFF3470
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 217,429 + GFF3465 0.40 +0.3 217,548 + GFF3465 0.50 -0.7 217,548 + GFF3465 0.50 +4.5 218,068 - +0.7 218,467 + GFF3466 0.29 -1.3 218,762 - GFF3466 0.66 +0.1 218,905 + GFF3466 0.84 -1.2 218,905 + GFF3466 0.84 -0.6 218,905 + GFF3466 0.84 -4.1 218,905 + GFF3466 0.84 -1.5 218,906 - GFF3466 0.84 -0.5 219,033 - -2.5 219,151 + GFF3467 0.31 +1.7 219,365 - GFF3467 0.79 +0.7 219,618 + -0.8 219,665 + GFF3469 0.24 +0.5 219,666 - GFF3469 0.25 +0.3 219,666 - GFF3469 0.25 +0.2 219,666 - GFF3469 0.25 +0.3 219,702 - GFF3469 0.50 -0.2 219,834 + GFF3470 0.12 -0.2 219,851 - GFF3470 0.15 -1.0
Or see this region's nucleotide sequence