Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3366 and GFF3367 are separated by 137 nucleotides GFF3367 and GFF3368 are separated by 205 nucleotides GFF3368 and GFF3369 are separated by 71 nucleotides
GFF3366 - Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70), at 134,183 to 135,142
GFF3366
GFF3367 - Putative inner membrane lipoprotein, at 135,280 to 136,041
GFF3367
GFF3368 - FIG01046221: hypothetical protein, at 136,247 to 136,471
GFF3368
GFF3369 - FIG146278: Maf/YceF/YhdE family protein, at 136,543 to 137,127
GFF3369
Position (kb) 135
136
137 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 134.355 kb on - strand, within GFF3366 at 134.486 kb on - strand, within GFF3366 at 134.486 kb on - strand, within GFF3366 at 134.746 kb on + strand, within GFF3366 at 134.919 kb on + strand, within GFF3366 at 134.923 kb on + strand, within GFF3366 at 134.923 kb on + strand, within GFF3366 at 134.924 kb on - strand, within GFF3366 at 134.924 kb on - strand, within GFF3366 at 134.931 kb on - strand, within GFF3366 at 135.013 kb on + strand, within GFF3366 at 135.141 kb on - strand at 135.162 kb on - strand at 135.162 kb on - strand at 135.188 kb on - strand at 135.309 kb on + strand at 135.309 kb on + strand at 135.328 kb on + strand at 135.429 kb on + strand, within GFF3367 at 135.490 kb on + strand, within GFF3367 at 135.502 kb on + strand, within GFF3367 at 135.503 kb on - strand, within GFF3367 at 135.503 kb on - strand, within GFF3367 at 135.512 kb on + strand, within GFF3367 at 135.512 kb on + strand, within GFF3367 at 135.512 kb on + strand, within GFF3367 at 135.568 kb on - strand, within GFF3367 at 135.707 kb on + strand, within GFF3367 at 136.040 kb on - strand at 136.138 kb on + strand at 136.167 kb on - strand at 136.325 kb on - strand, within GFF3368 at 136.438 kb on - strand, within GFF3368 at 136.480 kb on + strand at 136.485 kb on - strand at 136.716 kb on - strand, within GFF3369 at 136.716 kb on - strand, within GFF3369 at 136.716 kb on - strand, within GFF3369 at 136.781 kb on - strand, within GFF3369 at 136.801 kb on - strand, within GFF3369 at 136.802 kb on + strand, within GFF3369 at 136.803 kb on - strand, within GFF3369
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 134,355 - GFF3366 0.18 -2.4 134,486 - GFF3366 0.32 +0.3 134,486 - GFF3366 0.32 -0.3 134,746 + GFF3366 0.59 -0.5 134,919 + GFF3366 0.77 -1.4 134,923 + GFF3366 0.77 +0.6 134,923 + GFF3366 0.77 -1.8 134,924 - GFF3366 0.77 -0.2 134,924 - GFF3366 0.77 -0.7 134,931 - GFF3366 0.78 -0.3 135,013 + GFF3366 0.86 +2.9 135,141 - -0.9 135,162 - +2.3 135,162 - -2.6 135,188 - +0.9 135,309 + -0.8 135,309 + -2.1 135,328 + -0.0 135,429 + GFF3367 0.20 -0.4 135,490 + GFF3367 0.28 -0.2 135,502 + GFF3367 0.29 +0.4 135,503 - GFF3367 0.29 -0.4 135,503 - GFF3367 0.29 -0.2 135,512 + GFF3367 0.30 -3.2 135,512 + GFF3367 0.30 -0.3 135,512 + GFF3367 0.30 +1.2 135,568 - GFF3367 0.38 -2.7 135,707 + GFF3367 0.56 -0.3 136,040 - -1.6 136,138 + -0.6 136,167 - -0.1 136,325 - GFF3368 0.35 +0.5 136,438 - GFF3368 0.85 -1.4 136,480 + +0.2 136,485 - +0.4 136,716 - GFF3369 0.30 -1.9 136,716 - GFF3369 0.30 -0.8 136,716 - GFF3369 0.30 -1.0 136,781 - GFF3369 0.41 +0.1 136,801 - GFF3369 0.44 -0.5 136,802 + GFF3369 0.44 -2.7 136,803 - GFF3369 0.44 +1.1
Or see this region's nucleotide sequence